ID A0A067NLG6_PLEOS Unreviewed; 790 AA.
AC A0A067NLG6;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=PLEOSDRAFT_1047347 {ECO:0000313|EMBL:KDQ24446.1};
OS Pleurotus ostreatus PC15.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus.
OX NCBI_TaxID=1137138 {ECO:0000313|EMBL:KDQ24446.1, ECO:0000313|Proteomes:UP000027073};
RN [1] {ECO:0000313|Proteomes:UP000027073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC15 {ECO:0000313|Proteomes:UP000027073};
RX PubMed=24958869; DOI=10.1073/pnas.1400592111;
RA Riley R., Salamov A.A., Brown D.W., Nagy L.G., Floudas D., Held B.W.,
RA Levasseur A., Lombard V., Morin E., Otillar R., Lindquist E.A., Sun H.,
RA LaButti K.M., Schmutz J., Jabbour D., Luo H., Baker S.E., Pisabarro A.G.,
RA Walton J.D., Blanchette R.A., Henrissat B., Martin F., Cullen D.,
RA Hibbett D.S., Grigoriev I.V.;
RT "Extensive sampling of basidiomycete genomes demonstrates inadequacy of the
RT white-rot/brown-rot paradigm for wood decay fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:9923-9928(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; KL198011; KDQ24446.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067NLG6; -.
DR STRING; 1137138.A0A067NLG6; -.
DR VEuPathDB; FungiDB:PLEOSDRAFT_1047347; -.
DR HOGENOM; CLU_004585_4_1_1; -.
DR InParanoid; A0A067NLG6; -.
DR OrthoDB; 208346at2759; -.
DR Proteomes; UP000027073; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000027073}.
FT DOMAIN 58..362
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 363..655
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 79..86
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 790 AA; 87930 MW; F8122B912F0F8B0F CRC64;
MHDENAPRRA ATARLQHRLH LVRPSTSICP QDHGLELPGG SEHRSGERLN ASVPLQLTLT
GSVVSAVQHP PQVPLQILAG PGSGKTRVLT SRIAHLILHH HIPPTAICAV TFTNKASNEM
RERLTKLIGT ERTRALKLGT FHAICALFLR KHALVVGLSN NFSVCDADES KKLISRLLKP
YQTFLEARDI TLKEATVFSM ISKHKYKGNT STDVIAELRI TKSKSDKENT GVQHKSRHIT
DEINSVVAEV YREYERALRK SNSLDFDDLL LFGLQLFTDH KESAGWCRHV LVDEFQDTNS
IQYELMKTLA VSADQCLTVV GDPDQSIYGW RSADVTNLAK MKKEADFPST ESILLEENYR
STASILKASL AIVSQDKARL PKSLHTSHPS GSIPSLVSFA TDQDEAAFIA HEIKRTAANM
GGILKWKDFA VLLRFNALSR VIESALQKSG IPCRILGGHK FFERMEIKDL LAYLQLVDNP
QFTPAFSRVI NVPARGIGAK TITELELRAQ KTNKTPLELV ELIHGGRVPD IKPPIKRKIP
SFVKTIQTLR DLAIDGSSPV DLIRRLVDLI GYEQHLKQAQ PQDWESRWEN VQELITFASE
VEGDIIEMDD SEETRSTTPL RMFLQASMLS SEGDQSNGED SNDKVTISTC HAAKGLEWPV
VIVPSVEHGT FPFYRCDDVE EERRLLYVAC TRAQCLLYLS HCGKRMVAGE TKSVELSGFV
SSVHHQDRTI FSDRAPELLP ADRAVISRVL GRPEPKHVEV AKHVAELCVP ASLICFTSLI
IGVQQPNGTP
//
