ID A0A067NYP0_PLEOS Unreviewed; 884 AA.
AC A0A067NYP0;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=alpha-1,2-Mannosidase {ECO:0000256|RuleBase:RU361193};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361193};
GN ORFNames=PLEOSDRAFT_1038751 {ECO:0000313|EMBL:KDQ29257.1};
OS Pleurotus ostreatus PC15.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus.
OX NCBI_TaxID=1137138 {ECO:0000313|EMBL:KDQ29257.1, ECO:0000313|Proteomes:UP000027073};
RN [1] {ECO:0000313|Proteomes:UP000027073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC15 {ECO:0000313|Proteomes:UP000027073};
RX PubMed=24958869; DOI=10.1073/pnas.1400592111;
RA Riley R., Salamov A.A., Brown D.W., Nagy L.G., Floudas D., Held B.W.,
RA Levasseur A., Lombard V., Morin E., Otillar R., Lindquist E.A., Sun H.,
RA LaButti K.M., Schmutz J., Jabbour D., Luo H., Baker S.E., Pisabarro A.G.,
RA Walton J.D., Blanchette R.A., Henrissat B., Martin F., Cullen D.,
RA Hibbett D.S., Grigoriev I.V.;
RT "Extensive sampling of basidiomycete genomes demonstrates inadequacy of the
RT white-rot/brown-rot paradigm for wood decay fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:9923-9928(2014).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR601382-2};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC {ECO:0000256|ARBA:ARBA00007658, ECO:0000256|RuleBase:RU361193}.
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DR EMBL; KL198007; KDQ29257.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067NYP0; -.
DR STRING; 1137138.A0A067NYP0; -.
DR VEuPathDB; FungiDB:PLEOSDRAFT_1038751; -.
DR HOGENOM; CLU_003818_2_1_1; -.
DR InParanoid; A0A067NYP0; -.
DR OrthoDB; 942598at2759; -.
DR Proteomes; UP000027073; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:UniProt.
DR CDD; cd00538; PA; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR044674; EDEM1/2/3.
DR InterPro; IPR001382; Glyco_hydro_47.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR036026; Seven-hairpin_glycosidases.
DR PANTHER; PTHR45679:SF5; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 1; 1.
DR PANTHER; PTHR45679; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2; 1.
DR Pfam; PF01532; Glyco_hydro_47; 1.
DR Pfam; PF02225; PA; 1.
DR PRINTS; PR00747; GLYHDRLASE47.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR601382-2};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycosidase {ECO:0000256|RuleBase:RU361193};
KW Hydrolase {ECO:0000256|RuleBase:RU361193, ECO:0000313|EMBL:KDQ29257.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601382-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000027073};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..884
FT /note="alpha-1,2-Mannosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001642723"
FT DOMAIN 732..797
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT ACT_SITE 143
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 284
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 379
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 398
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT BINDING 484
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-2"
SQ SEQUENCE 884 AA; 98691 MW; D4E66D13FB864059 CRC64;
MLWKYWPEAL FAGVLISLRC LAWGTDSSAT NLSTSSYDPW TTQRKIAVRE RTRQLWYHGF
DNYMTFAFPL DELSPLSCTG RGPDWDNPSN IATNDVAGNF SLTLIDVLDT LVVLNDRPRF
EIAVRNVIDW VNFDVNTKPQ VFETTIRVLG GLLSGHIFAS QTGQPFHLPW YKGELLKMAK
DLGERLLPAF ATPTGMPYAR VNLRHGVMKG ETQETCTAGA GSLILEFATL SRLTGDDRFE
KAAYKAFFAL WNRKSDIGLV GNTINTFSGL WSHPEITGIG AGIDSFYEYA LKWYILIGEV
EFLDVWKDSY AALMRYSRAV DGYWYRNVNI HSGDVSYYTI DSLSAFWPGL QVLSGDVQNA
IKSHMMYWNL WRAHSGLPEV YDTNFKTATS FQYPLRPELA ESTWYLYRAT RDTFYLDVGE
RIFEDLENRA KVGCGLTGIA DLRTNKRDDR MESFALSETL KYLYLLFDEE NPLHSDDSNY
VFTTEGHLLT LPRHLLRPVP PARLQFRQNE SHQCPAYHPF VHSYPNQKKP KRGRGLTLGI
RSRSDIDYSR ELLALLPDSA DRDASSPDGW CEQPKVETYS YDFILSADGQ ATPEDPLPSQ
LKLAAISGGY IIHNITGIRT HIVRRLDGKG YDITKLGPHA VKTGQIVYIN DTALFGPKKD
VEQTPLPPAP HSDGVVPLRF FVDLMDPTLR ANTIDGSSEL DAVMTAWTST FGGKLVSEPG
SEPIQFAHGE GVVVASEDSN LLGCNPYERD FDGAAVLVRR GECTFVEKLL HAKSAGASGV
IVISDIESRL NPSADAEDLD QAGDISDVAL VVLPPSSGFV VDQLLKLTES TGMGRLVVTL
VPEGQPAMHD EGHIPAELPS RTQQTKKRIL YLNGHPLVNT QLLN
//
