ID A0A068A1P5_9CAUD Unreviewed; 380 AA.
AC A0A068A1P5;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Putative transposase IS605 OrfB {ECO:0000313|EMBL:AHZ95254.1};
GN ORFNames=Tt72P_017 {ECO:0000313|EMBL:AHZ95254.1};
OS Thermus phage T72.
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes.
OX NCBI_TaxID=1416020 {ECO:0000313|EMBL:AHZ95254.1, ECO:0000313|Proteomes:UP000260464};
RN [1] {ECO:0000313|EMBL:AHZ95254.1, ECO:0000313|Proteomes:UP000260464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24786823; DOI=10.1016/j.jbiotec.2014.04.015;
RA Stefanska A., Kaczorowska A.K., Plotka M., Fridjonsson O.H.,
RA Hreggvidsson G.O., Hjorleifsdottir S., Kristjansson J.K., Dabrowski S.,
RA Kaczorowski T.;
RT "Discovery and characterization of RecA protein of thermophilic bacterium
RT Thermus thermophilus MAT72 phage Tt72 that increases specificity of a PCR-
RT based DNA amplification.";
RL J. Biotechnol. 182-183:1-10(2014).
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000256|ARBA:ARBA00011044}.
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DR EMBL; KF723224; AHZ95254.1; -; Genomic_DNA.
DR Proteomes; UP000260464; Genome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR InterPro; IPR021027; Transposase_put_HTH.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR NCBIfam; TIGR01766; IS200/IS605 family accessory protein TnpB-like domain; 1.
DR PANTHER; PTHR30405:SF20; RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED; 1.
DR PANTHER; PTHR30405; TRANSPOSASE; 1.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Transposition {ECO:0000256|ARBA:ARBA00022578};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 1..46
FT /note="Transposase putative helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12323"
FT DOMAIN 168..281
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 293..361
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
FT COILED 16..54
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 209..253
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 380 AA; 44221 MW; D73E8529113F2275 CRC64;
MLKAFKYRLY PTKPQIKDLE RTLELCRQLY NAVLQERRDA YKKAKKTISA YEQNNHLPEI
RAEIPEYKHV HSQVLQDVIK RVDLAFKGFF RRLKERRKAG YPRFKGEKRY DSFTFPQAGT
TGVKLQKDGK RVLIHGIGSV KIKLHRPLEG KIKTATIKRE GKDWYIVFVC EVDPKPLPPN
DKQIGIDLGT NPYFLVTSEG EKIEAPNHYQ RTQRKLASIQ KELSKKKKNS YRFRQIREKL
AKLHRKIANQ RKDFHHKLAR RLVNKYGTIV HEDLNVEGLM RSYIAKGVQD AGWAQFLQIL
AYKAEEAGRQ VIKVDPKYTS QDCPVCGYRE KKPLWIREYT CSQCGTHLHR DVAAAINILT
RARTEPAGSV ISHEPQSPTF
//
