ID A0A068EUN7_GOSHI Unreviewed; 490 AA.
AC A0A068EUN7;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=LOC107951145 {ECO:0000313|RefSeq:XP_016741568.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|EMBL:AID55372.1};
RN [1] {ECO:0000313|EMBL:AID55372.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Hypocotyl {ECO:0000313|EMBL:AID55372.1};
RA Peng L., Tang J., Xia T.;
RT "Identification and functional analysis of the cotton GH9 genes.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [3] {ECO:0000313|RefSeq:XP_016741568.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016741568.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; KJ621645; AID55372.1; -; mRNA.
DR RefSeq; NP_001314514.1; NM_001327585.1.
DR RefSeq; XP_016741568.1; XM_016886079.1.
DR STRING; 3635.A0A068EUN7; -.
DR PaxDb; 3635-A0A068EUN7; -.
DR GeneID; 107951145; -.
DR KEGG; ghi:107951145; -.
DR OMA; EFGSSMQ; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000189702; Chromosome 8.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 26..490
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5010593664"
FT DOMAIN 29..483
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 410
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 461
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 470
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 490 AA; 53603 MW; 6D898BCED1E2F413 CRC64;
MAAPITMLIL CTSFLSLSLL STAFTSRDYA DALEKSILFF EGQRSGPLPS NQRLKWRGNS
GLSDGASYHV DLVGGYYDAG DNVKFGLPMA FTTTMLAWSV IEFGGSMHSQ IGNAKTALRW
ATDYLLKAAT ATPDSLYVQV GDPNMDHKCW ERPEDMDTPR NVYKVSAQNP GSDVAAETAA
ALAAASIVFK DSDPSYSAKL LQTAKNVFDF ADKYRGSYSD SLNSMVCPFY CSYSGYQDEL
LWGASWMHRA TQDSSYLSYI QSNGHTMGSD NDDFSFSWDD KRAGTKVLLS KDFLEKSTSE
FQLYKSHSDN YICSLIPGSS GFQAQYTPGG LLYKASESNL QYVTTTTFLL LTYAKYLSTN
GGVVTCGTST VTAESLIAQA KKQVDYILGD NPAKMSYMVG FGERYPQHVH HRGSSVPSIK
AHPDRINCNN GFQYLYANSP NPNVLVGAVI GGPDSKDNFA DDRNNYQQSE PATYINAPFV
GALAFFSGKA
//
