ID A0A068V8Q5_COFCA Unreviewed; 597 AA.
AC A0A068V8Q5;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 28-JUN-2023, entry version 27.
DE RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN ORFNames=GSCOC_T00019441001 {ECO:0000313|EMBL:CDP16869.1};
OS Coffea canephora (Robusta coffee).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC Bertiereae - Coffeeae clade; Coffeeae; Coffea.
OX NCBI_TaxID=49390 {ECO:0000313|EMBL:CDP16869.1, ECO:0000313|Proteomes:UP000295252};
RN [1] {ECO:0000313|Proteomes:UP000295252}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DH200-94 {ECO:0000313|Proteomes:UP000295252};
RX PubMed=25190796; DOI=10.1126/science.1255274;
RA Denoeud F., Carretero-Paulet L., Dereeper A., Droc G., Guyot R.,
RA Pietrella M., Zheng C., Alberti A., Anthony F., Aprea G., Aury J.M.,
RA Bento P., Bernard M., Bocs S., Campa C., Cenci A., Combes M.C.,
RA Crouzillat D., Da Silva C., Daddiego L., De Bellis F., Dussert S.,
RA Garsmeur O., Gayraud T., Guignon V., Jahn K., Jamilloux V., Joet T.,
RA Labadie K., Lan T., Leclercq J., Lepelley M., Leroy T., Li L.T.,
RA Librado P., Lopez L., Munoz A., Noel B., Pallavicini A., Perrotta G.,
RA Poncet V., Pot D., Priyono X., Rigoreau M., Rouard M., Rozas J.,
RA Tranchant-Dubreuil C., VanBuren R., Zhang Q., Andrade A.C., Argout X.,
RA Bertrand B., de Kochko A., Graziosi G., Henry R.J., Jayarama X., Ming R.,
RA Nagai C., Rounsley S., Sankoff D., Giuliano G., Albert V.A., Wincker P.,
RA Lashermes P.;
RT "The coffee genome provides insight into the convergent evolution of
RT caffeine biosynthesis.";
RL Science 345:1181-1184(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072}.
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DR EMBL; HG739223; CDP16869.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A068V8Q5; -.
DR STRING; 49390.A0A068V8Q5; -.
DR EnsemblPlants; CDP16869; CDP16869; GSCOC_T00019441001.
DR Gramene; CDP16869; CDP16869; GSCOC_T00019441001.
DR InParanoid; A0A068V8Q5; -.
DR OMA; GFDKYYN; -.
DR PhylomeDB; A0A068V8Q5; -.
DR Proteomes; UP000295252; Chromosome 11.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF41; ENDOGLUCANASE 5; 1.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Reference proteome {ECO:0000313|Proteomes:UP000295252};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..28
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 29..597
FT /note="cellulase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5018595358"
FT DOMAIN 505..587
FT /note="Carbohydrate binding"
FT /evidence="ECO:0000259|SMART:SM01063"
SQ SEQUENCE 597 AA; 66115 MW; B1DDF8CB550326D1 CRC64;
MAKLGGSQAL LAMLVGILVF QAACRVSCFD YGDALDKTLL FLEAQRSGKL PPNQRVKWRG
DSGLKDGFLQ GVNLVGGYYD AGDHVKFGLP MAFTMTMLSW GAIEYRNEIV RLNQMGHTLA
AIKWGTDYFI KAHPQPNVLW GQVGDGVSDH YCWERAEDMT TPRTAYKLDP EHPGSDLAGE
TAAALAAASL AFKPYNSSYS SLLLVHAKQL FSFADSFRGK YDDSIQCAKQ FYTSSGYSDE
LLWAATWLYR ATDDEYYLKY VVDNAVPMGG TGWAVREFSW DTKYAGIQIL LSKVLLDGAG
RAYTSTLQQY QAKADFFACA CMQKNDGYNV ATTPGGLVYV REWNNMQYAA SAAFLLAPKS
NLRISSADYF LGKNPKSMSY VVGYGQNYPI HVHHRGSSIA SVFALQSVVG CVQGFESWYR
RPEPNPNVIY GALVGGPNNN DAFFDDRSNY EQTEPTISGV APLFGLFSRL QSLSGNSGYH
APVSQKPNPN TPVTTQNLLH ADVPVHFFHS ITQSWTIGKM TYYRHKVIIK NTSQKPITDL
KLKFENLTGS LWGLNPTQEK DVYELPQWLK VLKPGSDCTV VYVQGGAQAK VSVPSYH
//
