ID A0A069CWW4_WEIOS Unreviewed; 437 AA.
AC A0A069CWW4;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000256|HAMAP-Rule:MF_01074};
GN ORFNames=WOSG25_180060 {ECO:0000313|EMBL:GAK31857.1};
OS Weissella oryzae (strain DSM 25784 / JCM 18191 / LMG 30913 / SG25).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; Weissella.
OX NCBI_TaxID=1329250 {ECO:0000313|EMBL:GAK31857.1, ECO:0000313|Proteomes:UP000030643};
RN [1] {ECO:0000313|Proteomes:UP000030643}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 25784 / JCM 18191 / LMG 30913 / SG25
RC {ECO:0000313|Proteomes:UP000030643};
RX PubMed=25013139; DOI=10.1128/genomea.00667-14;
RA Tanizawa Y., Fujisawa T., Mochizuki T., Kaminuma E., Suzuki Y.,
RA Nakamura Y., Tohno M.;
RT "Draft genome sequence of Weissella oryzae SG25T, isolated from fermented
RT rice grains.";
RL Genome Announc. 2:0-0(2014).
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000256|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; DF820501; GAK31857.1; -; Genomic_DNA.
DR RefSeq; WP_027699777.1; NZ_DF820501.1.
DR AlphaFoldDB; A0A069CWW4; -.
DR STRING; 1329250.WOSG25_180060; -.
DR eggNOG; COG1641; Bacteria.
DR OrthoDB; 9765625at2; -.
DR Proteomes; UP000030643; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.1380; Transcriptional regulatory protein pf0864 domain like; 1.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR NCBIfam; TIGR00299; nickel pincer cofactor biosynthesis protein LarC; 1.
DR PANTHER; PTHR36566; NICKEL INSERTION PROTEIN-RELATED; 1.
DR PANTHER; PTHR36566:SF1; NICKEL INSERTION PROTEIN-RELATED; 1.
DR Pfam; PF01969; Ni_insertion; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_01074};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|HAMAP-Rule:MF_01074};
KW Reference proteome {ECO:0000313|Proteomes:UP000030643}.
FT REGION 85..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 437 AA; 48055 MW; 8D8FDE9F8713E390 CRC64;
MKTLYLEPFS GISGDMLIGA LLDLGVDFAS FKAALAKLEL TGYELNMRSE KKSAINGTSF
HVSLTDSAHS IDEGLTLAEQ DGLATEPVHH HHHHSHAEDE VHEHHHHGRN LAEIQAIINA
SDLSEKVKAG ALATFEEIAR AEAKVHGLPL EAVHFHEVGA IDSIVDIVGA FIALDLLGVD
QVISGELVDG TGTIKVAHGV MPVPVPAVMM MRQGTDIPIR QRTDVQTELI TPTGLALVKV
LVTQFTSMPS QQVLVNTGYG FGSRDTGSLN ALRVHLFDSK QSEREVKQQD ETVLELHTNL
DDTTGEQLAF VMNELMEAGV YDVFFTPIFM KKGRPAYELT VILSEQFRAR VTELLFEHTT
TIGLRWSVMQ RTIMQRSFNT VITSYGKLKV KVLTYNAIKK YSFEADELSA LARKAGKSII
EIEAAGRLAL ENPKEEV
//
