ID A0A071LNT5_9ENTR Unreviewed; 334 AA.
AC A0A071LNT5;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN ORFNames=DT73_25450 {ECO:0000313|EMBL:KEA50218.1};
OS Mangrovibacter sp. MFB070.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Mangrovibacter.
OX NCBI_TaxID=1224318 {ECO:0000313|EMBL:KEA50218.1, ECO:0000313|Proteomes:UP000027726};
RN [1] {ECO:0000313|EMBL:KEA50218.1, ECO:0000313|Proteomes:UP000027726}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MFB070 {ECO:0000313|EMBL:KEA50218.1,
RC ECO:0000313|Proteomes:UP000027726};
RA Joseph T.C., Varghese A.M., Baby A., Reghunathan D., V M., Lalitha K.V.;
RT "Draft Genome Sequence of Mangrovibacter spp. MFB070 a Nitrogen-fixing
RT Bacterium Isolated from an Aquaculture Farm.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Efflux system for nickel and cobalt.
CC {ECO:0000256|ARBA:ARBA00002510}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA
CC subfamily. {ECO:0000256|ARBA:ARBA00010428}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEA50218.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JJMI01000053; KEA50218.1; -; Genomic_DNA.
DR RefSeq; WP_036116381.1; NZ_JJMI01000053.1.
DR AlphaFoldDB; A0A071LNT5; -.
DR eggNOG; COG2215; Bacteria.
DR Proteomes; UP000027726; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR Pfam; PF03824; NicO; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW Nickel {ECO:0000256|ARBA:ARBA00022596};
KW Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW Reference proteome {ECO:0000313|Proteomes:UP000027726};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU362101};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU362101};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT TRANSMEM 17..36
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 113..135
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 155..174
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 251..278
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 299..320
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
SQ SEQUENCE 334 AA; 36490 MW; 9535AE20F86ADBD3 CRC64;
MSLIFTPRSQ KVRGRDLWPL FVFVIGLVVA CAWLWANWSL VLLQSAAWQR ELNQLVSQLL
RGIASTPVKS GVMLVGVSFL YGVLHALGPG HGKVVIATWL ATNPAKLKTS LRMTLAASLL
QGLVAVMLVF VVLYLLGLPS RQLHYSHYLL EKASFIVVTL LGVMLCLRAV RQLWRTLKHP
LRFKRLYPAG STTPAHIHSA HCGCGHQHMP LPEQVSQATT WREKALVIGS MGMRPCSGAI
MVLLFSKVAG VFFWGIVAAM AMAAGTALTI SLLAFLTHGC RALAQRLVRE RGVVYWQQTA
WATLALAGGV VLCVCGIVLW QSTELVLPGL RGRG
//
