ID A0A072PEL7_9EURO Unreviewed; 628 AA.
AC A0A072PEL7;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
GN ORFNames=A1O9_09810 {ECO:0000313|EMBL:KEF54015.1};
OS Exophiala aquamarina CBS 119918.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala.
OX NCBI_TaxID=1182545 {ECO:0000313|EMBL:KEF54015.1, ECO:0000313|Proteomes:UP000027920};
RN [1] {ECO:0000313|EMBL:KEF54015.1, ECO:0000313|Proteomes:UP000027920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 119918 {ECO:0000313|EMBL:KEF54015.1,
RC ECO:0000313|Proteomes:UP000027920};
RG The Broad Institute Genomics Platform;
RA Cuomo C., de Hoog S., Gorbushina A., Walker B., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Exophiala aquamarina CBS 119918.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEF54015.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AMGV01000011; KEF54015.1; -; Genomic_DNA.
DR RefSeq; XP_013256605.1; XM_013401151.1.
DR AlphaFoldDB; A0A072PEL7; -.
DR STRING; 1182545.A0A072PEL7; -.
DR GeneID; 25284718; -.
DR VEuPathDB; FungiDB:A1O9_09810; -.
DR HOGENOM; CLU_015599_1_0_1; -.
DR OrthoDB; 460053at2759; -.
DR Proteomes; UP000027920; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Reference proteome {ECO:0000313|Proteomes:UP000027920}.
FT DOMAIN 36..290
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
SQ SEQUENCE 628 AA; 70613 MW; 9009D5ACF1E6A10D CRC64;
MTVRQSAVQV TDLLTGVKKL WETSLLKKAC SAPTRIGVCV SGGPDSMALA YLLSRIPQVD
PSIRIEPIAF IVNHNARPGS RLEAERVGTQ LRTHGKAPMA SQDISTETPI DIESRILHMR
WPEETNPLAL PDFEMMARYS RYQLIAQAAI REKIQHLFLG HHLDDQLETI IMRLVRNTSF
SFLAIKGMAE TSRIPCCENI RGANESLDFP SADGHLPPDK APQQETESWP FAANYGSPVG
ISELHGLQLH RPLLEFKKSQ LIGTCQQHKV QYVSDETNFD PTITMRNAVR YMRAKFNLPR
ALQATSLLNL MAAAREHSRS LESRGNELAS HFQIVEFDLR VGSMIVQLPQ DLASVCERDL
EGTSHALSSL TSVISPQPRD ATPSLVPQAR CLEFISILSS IRPQIPVSKK SGEAISTRQT
LQGATLQIQQ TQIEQLANTP GMGPGQSRWR LSRPTMRYDE IKAVEQKFEP EFGNHEGDSS
GAWSKWILWD HRYWIRVRSR IATDLSHVAI RNFRREDVHC IVSKGPPNWR NFKSTLREVA
PGNMRWTLPI LTFADEISAF PTLNVRVQNS ANGRGEGGDL PAHPPVLEWE MCYKVIDKPF
IQAQKSKIVW KNLADAYDSN QRRAFVSA
//
