ID A0A073KAG7_9BACI Unreviewed; 562 AA.
AC A0A073KAG7;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=Alpha-amylase {ECO:0000256|RuleBase:RU361134};
DE EC=3.2.1.1 {ECO:0000256|RuleBase:RU361134};
GN ORFNames=BAMA_23260 {ECO:0000313|EMBL:KEK19298.1};
OS Bacillus manliponensis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=574376 {ECO:0000313|EMBL:KEK19298.1, ECO:0000313|Proteomes:UP000027822};
RN [1] {ECO:0000313|EMBL:KEK19298.1, ECO:0000313|Proteomes:UP000027822}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 15802 {ECO:0000313|EMBL:KEK19298.1,
RC ECO:0000313|Proteomes:UP000027822};
RA Lai Q., Liu Y., Shao Z.;
RT "Draft genome sequence of Bacillus manliponensis JCM 15802 (MCCC
RT 1A00708).";
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC polysaccharides containing three or more (1->4)-alpha-linked D-
CC glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|RuleBase:RU361134};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEK19298.1}.
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DR EMBL; JOTN01000008; KEK19298.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A073KAG7; -.
DR STRING; 574376.BAMA_23260; -.
DR eggNOG; COG0366; Bacteria.
DR Proteomes; UP000027822; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd11333; AmyAc_SI_OligoGlu_DGase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR006046; Alpha_amylase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR032091; Malt_amylase_C.
DR InterPro; IPR045857; O16G_dom_2.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR PANTHER; PTHR10357:SF178; OLIGO-1,6-GLUCOSIDASE 3-RELATED; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF16657; Malt_amylase_C; 1.
DR PRINTS; PR00110; ALPHAAMYLASE.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361134};
KW Glycosidase {ECO:0000256|RuleBase:RU361134};
KW Hydrolase {ECO:0000256|RuleBase:RU361134};
KW Reference proteome {ECO:0000313|Proteomes:UP000027822};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 21..423
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 562 AA; 66025 MW; A1EB74CFA14B420D CRC64;
MHEERDNMMN KTWWKEAVAY QIYPRSFMDS NGDGIGDLQG MISKLDYLKD LGIDVIWICP
MYKSPNDDNG YDISDYQEIM DEFGTMADFD QLLEEVHKRG MKLIIDLVIN HTSDEHPWFI
ESRSSKDNPK RDWYIWRDGK DGKEPNNWES IFNGSAWEYD EETDQYYLHL FSRKQPDLNW
ENEEVREALY DTVNWWLDKG IDGFRVDAIS HIKKEDGMKD MPNPKGLKYV SSFDKHMNVD
GIQPLLEELK ERTFSNYDIM TVGEANGVKI EDAELWVGEE NGKFNMVFQF EHLSLWDAEK
KKELDVVGLK KVLTKWQKGL ENKGWNALYI ENHDKPRIVS TWGDDAQYWR ESATALGALY
FFMHGTPFIY QGQEIGMTNV QFPNIEDYDD VAVKNLYREK IAEGVSHKEM MEIIWASCRD
NSRTPMQWNK EENAGFTTGT PWFGMNPNYK DINVEAQEAD SESILNFYKK MIRLKKENEV
FNYGTYDLLL EEDPQIYAYT RTLGEEKVIV ISNISKSEAV YEEEAFALEC NRLLLNNYEV
ANHEATTKIE LKPYETRVYR IS
//
