ID   A0A073KQ52_9BACI        Unreviewed;       713 AA.
AC   A0A073KQ52;
DT   01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2014, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE            EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE   AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE   AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN   ORFNames=BAGA_25500 {ECO:0000313|EMBL:KEK24508.1};
OS   Bacillus gaemokensis.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=574375 {ECO:0000313|EMBL:KEK24508.1, ECO:0000313|Proteomes:UP000027778};
RN   [1] {ECO:0000313|EMBL:KEK24508.1, ECO:0000313|Proteomes:UP000027778}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 15801 {ECO:0000313|EMBL:KEK24508.1,
RC   ECO:0000313|Proteomes:UP000027778};
RA   Lai Q., Liu Y., Shao Z.;
RT   "Draft genome sequence of Bacillus gaemokensis JCM 15801 (MCCC 1A00707).";
RL   Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC         amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC         of amylopectin and glycogen.; EC=3.2.1.41;
CC         Evidence={ECO:0000256|ARBA:ARBA00023965};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KEK24508.1}.
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DR   EMBL; JOTM01000006; KEK24508.1; -; Genomic_DNA.
DR   RefSeq; WP_033674275.1; NZ_LTAQ01000001.1.
DR   AlphaFoldDB; A0A073KQ52; -.
DR   SMR; A0A073KQ52; -.
DR   STRING; 574375.AZF08_05045; -.
DR   eggNOG; COG1523; Bacteria.
DR   OrthoDB; 9761875at2; -.
DR   Proteomes; UP000027778; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR   CDD; cd02860; E_set_Pullulanase; 1.
DR   Gene3D; 2.60.40.2320; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR049117; pulA_all-beta.
DR   InterPro; IPR040697; PulA_N1.
DR   InterPro; IPR011840; PulA_typeI.
DR   NCBIfam; TIGR02104; pulA_typeI; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21653; pulA_all-beta; 1.
DR   Pfam; PF17999; PulA_N1; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
PE   4: Predicted;
FT   DOMAIN          227..613
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   713 AA;  81571 MW;  8B1DD42E4DFCE4A6 CRC64;
     MLKVKRPFEA YLDEMNKITI LLPYAYGTSR TFVLHEGNQV WELPIAQTIS LPEATKYECY
     MERSLDVGKY YTVRDERDEE TDLQIGAVIR TAAFDEKYYY EAADLGACYK KEETTFKVWA
     PTARLAKIRI YKSDKEYTDY EMNRGENGVW SRVLQGNLDG AQYTFLVCIN LIWNEAVDPY
     AKSVSINGKY GIVIDLAKTY IKKQTTLPKL ESFADAILYE LHIRDATVHP NSGAEKKGMY
     TGLAEEDTKG KLGTATAFSY IKDLGVTHVE ILPLHHFAGV DETKPFASYN WGYNPLYYNV
     PTGVYATNPF DPYNRIVECK QLIEKFHEHG IRVILDVVYN HVYERETSSF EKLVPGYYFR
     HGENGMPSNG TGVGNDLASE RKMMRKFIMD SVLYWLTEYN IDGFRFDLMG ILDIETMNIL
     EKEVRARKKD ALLLGEGWEL QTPLLAEEKA TLRNAKKMPR IAQFNDQFRD AIKGSTFDVN
     KRGFAFGGSV DQKHLRYVLA GSLSHIKREG LFCEPVQSIN YVECHDNMTM WDKLVCSNQE
     TEDILKRRHC LATAMTILSQ GIPFLHAGQE FYRSKKGNEN SYNANDEINQ LDWDQKEKEL
     ETVAYIKGLI AIRKAHGALR LSTAESIEKH MNFLQVPPAS LAYHLKNVGA FGSWKEIVVL
     FHSGLQVETI ALPKEETWYV LANDRKANVQ PISSFQGKEL QMSPISTYIL AIM
//