ID A0A074N3R8_9SPHN Unreviewed; 565 AA.
AC A0A074N3R8;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=EH32_06905 {ECO:0000313|EMBL:KEO98828.1};
OS Erythrobacter litoralis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter.
OX NCBI_TaxID=39960 {ECO:0000313|EMBL:KEO98828.1, ECO:0000313|Proteomes:UP000027866};
RN [1] {ECO:0000313|EMBL:KEO98828.1, ECO:0000313|Proteomes:UP000027866}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 8509 {ECO:0000313|EMBL:KEO98828.1,
RC ECO:0000313|Proteomes:UP000027866};
RA Zheng Q.;
RT "A comprehensive comparison of genomes of Erythrobacter spp. Strains.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEO98828.1}.
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DR EMBL; JMIX01000003; KEO98828.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A074N3R8; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000027866; Unassembled WGS sequence.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR045443; DUF6504.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF20114; DUF6504; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000027866}.
FT DOMAIN 67..189
FT /note="UmuC"
FT /evidence="ECO:0000259|Pfam:PF00817"
FT DOMAIN 300..390
FT /note="DNA polymerase Y-family little finger"
FT /evidence="ECO:0000259|Pfam:PF11799"
FT DOMAIN 482..546
FT /note="DUF6504"
FT /evidence="ECO:0000259|Pfam:PF20114"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..25
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 565 AA; 61659 MW; AC55236B9A8C1A04 CRC64;
MTTDASAPTG PPAPKPPPTR TPGTPGHAPP RRILALWCAR LSVDRWRLGL GPEHACAPGE
GADAEPTALI TETAHGPRIA AVNHAGRAAG VAVGTMLADA RTVCPEVRTA PADPAGDLAF
LEKLALWARR WGPWSALDAP DGVLVDVTAV AHLFGGEARL LADVETACTT RGLAVRSAIA
PTAGAAWALA HYGPVRSIAR PEDDLLARLA ELPVAALRLD DDVLTVLRRL GLKRLGELAG
VAGSGADGES EAASRDAIQR RFRNRKSPAA NPLIRLDQLL GRVPEPLLPV AARAMPLVQR
RLVEPIRHRD LLDRVLADLA QDMARELEGR GEGARRLEIG LWRVDGEVIV RRLELAAATR
DPDHVIRLFG RKLDDVDAGF GVETVRLTAS WTEPLALEQG DIEQSAQQRG TSLAACIDRL
TVRLGAEAVR RPVPRASHLP ERATGWQGPL EPVPPAQGLL EFHQRPLKLL DRPERIAVLY
ASPDGFPQRF RWRGQVREVA RVEGPERIAP EWWRERSSAR LRDYYRIEDD AGRRYWIYRH
GIAGDGRGKD REGHAPDWFL QGLCT
//
