ID A0A074RVA1_9AGAM Unreviewed; 1050 AA.
AC A0A074RVA1;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Putative cytochrome P450 family:NADPH-P450 family reductase {ECO:0000313|EMBL:KEP51051.1};
GN ORFNames=V565_068390 {ECO:0000313|EMBL:KEP51051.1};
OS Rhizoctonia solani 123E.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Cantharellales; Ceratobasidiaceae; Rhizoctonia.
OX NCBI_TaxID=1423351 {ECO:0000313|EMBL:KEP51051.1, ECO:0000313|Proteomes:UP000027456};
RN [1] {ECO:0000313|EMBL:KEP51051.1, ECO:0000313|Proteomes:UP000027456}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=123E {ECO:0000313|EMBL:KEP51051.1,
RC ECO:0000313|Proteomes:UP000027456};
RA Cubeta M., Pakala S., Fedorova N., Thomas E., Dean R., Jabaji S., Neate S.,
RA Toda T., Tavantzis S., Vilgalys R., Bharathan N., Pakala S., Losada L.S.,
RA Zafar N., Nierman W.;
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000256|ARBA:ARBA00001971,
CC ECO:0000256|PIRSR:PIRSR000209-1};
CC -!- SIMILARITY: In the N-terminal section; belongs to the cytochrome P450
CC family. {ECO:0000256|ARBA:ARBA00010018}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEP51051.1}.
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DR EMBL; AZST01000197; KEP51051.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A074RVA1; -.
DR STRING; 1423351.A0A074RVA1; -.
DR HOGENOM; CLU_001570_7_0_1; -.
DR OrthoDB; 1691317at2759; -.
DR Proteomes; UP000027456; Unassembled WGS sequence.
DR GO; GO:0070330; F:aromatase activity; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0003958; F:NADPH-hemoprotein reductase activity; IEA:InterPro.
DR CDD; cd06206; bifunctional_CYPOR; 1.
DR CDD; cd11068; CYP120A1; 1.
DR Gene3D; 3.40.50.360; -; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR023206; Bifunctional_P450_P450_red.
DR InterPro; IPR003097; CysJ-like_FAD-binding.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR InterPro; IPR017927; FAD-bd_FR_type.
DR InterPro; IPR008254; Flavodoxin/NO_synth.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR023173; NADPH_Cyt_P450_Rdtase_alpha.
DR InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR19384:SF127; BIFUNCTIONAL CYTOCHROME P450_NADPH--P450 REDUCTASE; 1.
DR PANTHER; PTHR19384; NITRIC OXIDE SYNTHASE-RELATED; 1.
DR Pfam; PF00667; FAD_binding_1; 1.
DR Pfam; PF00258; Flavodoxin_1; 1.
DR Pfam; PF00175; NAD_binding_1; 1.
DR Pfam; PF00067; p450; 1.
DR PIRSF; PIRSF000209; Bifunctional_P450_P450R; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
DR PROSITE; PS51384; FAD_FR; 1.
DR PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Heme {ECO:0000256|PIRSR:PIRSR000209-1};
KW Iron {ECO:0000256|PIRSR:PIRSR000209-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000209-1};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000027456};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 490..631
FT /note="Flavodoxin-like"
FT /evidence="ECO:0000259|PROSITE:PS50902"
FT DOMAIN 669..892
FT /note="FAD-binding FR-type"
FT /evidence="ECO:0000259|PROSITE:PS51384"
FT BINDING 403
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000256|PIRSR:PIRSR000209-1"
SQ SEQUENCE 1050 AA; 115774 MW; 7A8F69E8C1EA13F2 CRC64;
MTTPIPQPPA IPFLGNVTDI DTELPSKSFT LLAKQYGEIF ALNMLGKSVV VVSSVKLAQE
VLDEKRFHKA LGTALVEVRN LVSDGLFTAY YGETNWGVAH RILMPAFGPL SIKSMFDDMF
DVISQLVLKW ERFGPEHDID PTDDFTRLAF ETIALCTFSY RLNTFYTEGE PPFVKAMGDF
LKECFLRSRR PTIVQALSYA SNAKYAADMD IMNNLANKIV KDRKDHPSDK KDLLNAMLLG
RDPQTGQGLS EENIKAQMLT FLIAGHETTS GMLSFAMAHI MKNPEVYAKI RNEVDTVLGK
DPIKFEHLSK LTYINAVLRE SLRFTPSIGQ YAVTPHEDEI VGDGKYLIKK DTIVVVSAGD
IGRDPAVWGE DADTFNPDRM LDGKFEALPP KAWIPFGNGA RACIGRPFAW QEALIAIATL
FQKFDFKPRD ESFTLQFKQT LTLKPKDFTF HAIPRKGAPS FSVAAASSQA TNGPTPGKEI
VGGESKGSPL YVFYGSNTGS CEGFAQNIAS EAAGKGFRAT IGTLNSTVGS LPKDGPVVIV
TASFEGEPAD NAGHFVEVLV NSTSAKDLEG VSFAVFGAGN HDWVHTYQRV PRLIDETLEK
KGAKRLLERG EGDAGGDRFT ESFDEWENKL WEALSKEYSV AAIEGSSAPS VEVKFVGAPT
DRAATLRQPD SRLGLVVENR LLTAAGAPAK RHIEFELPQD MTYQAGDYLA ILPSNPPEYV
RRVLARFKVS PEQEVVLNAA GPTTLPTGKP VSISEVLSGF VEIGQVASKR NISTLLEHTK
DATTRTDLET LITGFSVKDG HPTTFSMLDL LEKYSDIDLP LGKFIASLPT MRLRQYSISS
SPLWNPSHVT LTIGVVAQGQ FLGVASNYLA NLRKGDRVQM AVRPSSKAFH PPSDPNVPMV
LFAAGSGMAP FRGFLQERAM QKKAGREVAK CLLFFGCRKP DEDFLYSEAE LAEWKELGVV
DIRPTFSRAP EQSEGQKYVQ DRVWNDRELV REYYAKDAKF YTCGGNHVAT GIRDACVRII
AEGKEDADEN RIQEIWNKIQ AERYATDVFG
//
