ID A0A074WL35_9PEZI Unreviewed; 458 AA.
AC A0A074WL35;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|ARBA:ARBA00017788, ECO:0000256|RuleBase:RU368004};
DE EC=2.1.1.211 {ECO:0000256|ARBA:ARBA00012795, ECO:0000256|RuleBase:RU368004};
DE Flags: Fragment;
GN ORFNames=M436DRAFT_15984 {ECO:0000313|EMBL:KEQ73845.1};
OS Aureobasidium namibiae CBS 147.97.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Dothideales; Saccotheciaceae; Aureobasidium.
OX NCBI_TaxID=1043004 {ECO:0000313|EMBL:KEQ73845.1, ECO:0000313|Proteomes:UP000027730};
RN [1] {ECO:0000313|EMBL:KEQ73845.1, ECO:0000313|Proteomes:UP000027730}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 147.97 {ECO:0000313|EMBL:KEQ73845.1,
RC ECO:0000313|Proteomes:UP000027730};
RX PubMed=24984952;
RA Gostin Ar C., Ohm R.A., Kogej T., Sonjak S., Turk M., Zajc J., Zalar P.,
RA Grube M., Sun H., Han J., Sharma A., Chiniquy J., Ngan C.Y., Lipzen A.,
RA Barry K., Grigoriev I.V., Gunde-Cimerman N.;
RT "Genome sequencing of four Aureobasidium pullulans varieties:
RT biotechnological potential, stress tolerance, and description of new
RT species.";
RL BMC Genomics 15:549-549(2014).
CC -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00000660,
CC ECO:0000256|RuleBase:RU368004};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368004}.
CC -!- SIMILARITY: Belongs to the TRM44 family.
CC {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
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DR EMBL; KL584708; KEQ73845.1; -; Genomic_DNA.
DR RefSeq; XP_013427840.1; XM_013572386.1.
DR AlphaFoldDB; A0A074WL35; -.
DR STRING; 1043004.A0A074WL35; -.
DR GeneID; 25407891; -.
DR HOGENOM; CLU_018580_2_0_1; -.
DR OrthoDB; 1353816at2759; -.
DR Proteomes; UP000027730; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 2.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW Methyltransferase {ECO:0000256|RuleBase:RU368004};
KW Reference proteome {ECO:0000313|Proteomes:UP000027730};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW tRNA processing {ECO:0000256|RuleBase:RU368004}.
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KEQ73845.1"
FT NON_TER 458
FT /evidence="ECO:0000313|EMBL:KEQ73845.1"
SQ SEQUENCE 458 AA; 52380 MW; A9293B743FE61F8B CRC64;
WQPMLESDCT FEPEMFLRVM HNLIKNPNIM STHLFRADIL YDSDADHTII EGPTTTHFSD
FTKHLKPEYR PRYAELPGFK WERTYVRQLV PRNRQLDKDL PQTCHFYTRL DGETTRNLIL
YIPHVEDSEA MPWYHPRVRA VAFSHYWTPS SRLGAVAVHY SLFPDIPLDS RLERTALQLL
TKIHKHSRGQ QAGYQKRVHH DLVVPQKTFQ ETYARLKSTY AKFLIEGWAE VTDPAKHVFE
DLGIASFLIE LWKVMYDCTQ LADDKGNAVQ LEKDSTKPPF PGFVDIGCGN GLLVNILIKE
GFPGWGFDAR HRKSWETFAP NVKSKLAEGI LVPEQLEAVD LLRDRDFHNG RFKKGTFIVS
NHADELTPWT PLLAYLNDSP FIAIPCCSHN LAGARWRAPI SKAAKEKAAA QRPGTKNVQS
AYASLCDYVS GLANDMGFVV ETEILRIPST RNTCILGR
//
