ID A0A075JSC9_9BACI Unreviewed; 494 AA.
AC A0A075JSC9;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=X953_19340 {ECO:0000313|EMBL:AIF44981.1};
OS Virgibacillus sp. SK37.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Virgibacillus.
OX NCBI_TaxID=403957 {ECO:0000313|EMBL:AIF44981.1, ECO:0000313|Proteomes:UP000027985};
RN [1] {ECO:0000313|EMBL:AIF44981.1, ECO:0000313|Proteomes:UP000027985}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK37 {ECO:0000313|EMBL:AIF44981.1,
RC ECO:0000313|Proteomes:UP000027985};
RA Phrommao E., Yongsawatdigul J., Rodtong S., Steele J.L.;
RT "Complete genome sequence of Virgibacillus sp. SK37, a moderately
RT halophilic bacterium isolated from Thai fish sauce fermentation.";
RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP007161; AIF44981.1; -; Genomic_DNA.
DR RefSeq; WP_040956836.1; NZ_CP007161.1.
DR AlphaFoldDB; A0A075JSC9; -.
DR STRING; 403957.X953_19340; -.
DR REBASE; 89963; M.VspSK37ORF19340P.
DR KEGG; vir:X953_19340; -.
DR HOGENOM; CLU_013049_4_2_9; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000027985; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000027985};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..134
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 147..459
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 462..489
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 494 AA; 56441 MW; 1BF67DD917070B9D CRC64;
MSKLTLQQLE SHLWESANIL RGSIDSSDYK NYIFGLLFLK RLSDVFIETA KEIEREENDD
YGWYDRDEHQ FFVPERARWT HIQIQTQDIG NTINKAFEAL EEENPSLDGV LANIDFNDKD
KLPDKLLMQL VQHFSSIDLS NGNLSEPDML GRAYEYLIKQ FADDAGKKGG EFYTPTKVVE
LIVNLIKPEE GMRVCDPTVG SGGMLVQSVD YIKSQGGDPR NLTLHGQERN LNTWAICKMN
LLLHGLSDHR IERGDTIRDP KLLGDDKELL LYDRVIANPP FSLKNWGREE AEADEYGRFR
FGIPPKNAGD YGFVQHMVST LNHEGRAGVV MPHGVLFRGG AEGKIREGLL KEDLVEGIIG
LPSNLFYGTG IPACILILNR KKAEEHKGKI FILDGSKDHQ EGKNQNVLRS DDIEKIVQAY
DAWEDQNKYC RVVGLEEIEE NDYNLNIARY IDTTEEEEDI DVKAALTDLK KIEQERKEIE
EKMYGYLKEL GYDE
//
