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Database: UniProt
Entry: A0A078F3G6_BRANA
LinkDB: A0A078F3G6_BRANA
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ID   A0A078F3G6_BRANA        Unreviewed;       912 AA.
AC   A0A078F3G6;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   28-MAR-2018, entry version 16.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   Name=BnaC01g30560D {ECO:0000313|EMBL:CDY09035.1};
GN   ORFNames=GSBRNA2T00001176001 {ECO:0000313|EMBL:CDY09035.1};
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae;
OC   Brassica.
OX   NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY09035.1, ECO:0000313|Proteomes:UP000028999};
RN   [1] {ECO:0000313|EMBL:CDY09035.1, ECO:0000313|Proteomes:UP000028999}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX   PubMed=25146293; DOI=10.1126/science.1253435;
RA   Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X.,
RA   Chiquet J., Belcram H., Tong C., Samans B., Correa M., Da Silva C.,
RA   Just J., Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P.,
RA   Noel B., Labadie K., Alberti A., Charles M., Arnaud D., Guo H.,
RA   Daviaud C., Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H.,
RA   Tack D., Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G.,
RA   Renault V., Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H.,
RA   Chalabi S., Hu Q., Fan C., Tollenaere R., Lu Y., Battail C., Shen J.,
RA   Sidebottom C.H., Wang X., Canaguier A., Chauveau A., Berard A.,
RA   Deniot G., Guan M., Liu Z., Sun F., Lim Y.P., Lyons E., Town C.D.,
RA   Bancroft I., Wang X., Meng J., Ma J., Pires J.C., King G.J.,
RA   Brunel D., Delourme R., Renard M., Aury J.M., Adams K.L., Batley J.,
RA   Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W., Sharpe A.G.,
RA   Paterson A.H., Guan C., Wincker P.;
RT   "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT   Brassica napus oilseed genome.";
RL   Science 345:950-953(2014).
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
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DR   EMBL; LK031991; CDY09035.1; -; Genomic_DNA.
DR   EnsemblPlants; CDY09035; CDY09035; GSBRNA2T00001176001.
DR   Gramene; CDY09035; CDY09035; GSBRNA2T00001176001.
DR   OMA; SEEMETM; -.
DR   Proteomes; UP000028999; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000028999};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000028999}.
FT   DOMAIN      102    421       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      497    858       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   912 AA;  102627 MW;  5F2C5FEC2A5CD0B1 CRC64;
     MVGPTLFHRR KHSWPPEEFI SKTTLQLLDF DSAAPPSHAW RRRLNCHANI LKEFTITFRE
     AIKMVRLGIR LWSYVREEAS HGRKAPIDPF TKDNCRPSAS QGVPLGGMGS GSISRGFRGE
     FKQWQITPGT CDPSPMMSNQ FSIFISRENV HKKYASVLAP GQHGSLGKLR DKGISSWGWN
     LSGQHSTYHA LFPRAWTVYD GEPDPELKIS CRQISPFIPK NYRDSSLPAA VFVYTLVNTG
     KQRAKVSLVF TWANSIGGTS HMSGGHVNEP FIGEDGVSGV LLHHKTGMGN PPVTFAISAC
     ETQNVNVTVL PCFGLSEDSF FTAKDMWETM EKDGNFDQEN FNSGPSMPSS PGDTICAAVS
     ASAWVEAHGK CTVSFALSWP SPKVKFSKGS TYDRRYTKFY GTSRRAAVDL VHDALTHYKR
     WEEDIEAWQN PILRDDRLPE WYKFTLFNEL YFLVAGGTVW IDGKEQQNNL NNGDRNGIES
     NGLFVETSQG DDEDDVGRFL YLEGVEYVMW NTYDVHFYAS YALLMLFPKI ELNIQRDFAK
     AVLSEDTRKV KFLAEGNWGI RKVRGAVPHD LGMHDPWNEM NAYNIHDTSK WKDLNPKFVL
     QVYRDFAATG DHQFGIDVWP AVRAAMEYME QFDRDNDDLI ENDGFPDQTY DTWTVHGVSA
     YCGCLWLAAL QAAAAMALQV GDKFFAELCK NKFLNAKAAL EAKLWNGTYL NYDSGASSNS
     KSIQTDQLAG QWYTASSGLP PVFEDFKIRS TLQKIFDFNV MKTKGGRMGA VNGMHPDGKV
     DETCMQSREI WTGVTYAAAA TMILSGMEEQ GFTTAEGIFA AGWSEEGFGY WFQTPEGWTM
     DGHYRSLIYM RPLAIWGMQW ALTVPKAILD GPKINMIDRV HMSPRSRRFS HNVKVVKHKA
     KCFGNCKLSC SC
//
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