ID A0A078F5L1_BRANA Unreviewed; 454 AA.
AC A0A078F5L1;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE SubName: Full=BnaC03g54910D protein {ECO:0000313|EMBL:CDY08691.1};
GN Name=BnaC03g54910D {ECO:0000313|EMBL:CDY08691.1};
GN ORFNames=GSBRNA2T00000574001 {ECO:0000313|EMBL:CDY08691.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY08691.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY08691.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-cyano-L-alanine + 2 H2O = L-aspartate + NH4(+);
CC Xref=Rhea:RHEA:11188, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29991, ChEBI:CHEBI:77860; EC=3.5.5.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000322};
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC Nitrilase family. {ECO:0000256|ARBA:ARBA00008129}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LK031988; CDY08691.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078F5L1; -.
DR STRING; 3708.A0A078F5L1; -.
DR PaxDb; 3708-A0A078F5L1; -.
DR EnsemblPlants; CDY08691; CDY08691; GSBRNA2T00000574001.
DR Gramene; CDY08691; CDY08691; GSBRNA2T00000574001.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0047427; F:cyanoalanine nitrilase activity; IEA:UniProtKB-EC.
DR GO; GO:0000257; F:nitrilase activity; IBA:GO_Central.
DR GO; GO:0018822; F:nitrile hydratase activity; IBA:GO_Central.
DR GO; GO:0051410; P:detoxification of nitrogen compound; IBA:GO_Central.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR CDD; cd07564; nitrilases_CHs; 1.
DR Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR InterPro; IPR044149; Nitrilases_CHs.
DR PANTHER; PTHR46044; NITRILASE; 1.
DR PANTHER; PTHR46044:SF13; NITRILASE-RELATED; 1.
DR Pfam; PF00795; CN_hydrolase; 2.
DR SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 2.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
DR PROSITE; PS00920; NITRIL_CHT_1; 1.
DR PROSITE; PS00921; NITRIL_CHT_2; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000028999}.
FT DOMAIN 26..402
FT /note="CN hydrolase"
FT /evidence="ECO:0000259|PROSITE:PS50263"
FT ACT_SITE 66
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10139"
SQ SEQUENCE 454 AA; 49619 MW; 22014B98FCF3F08B CRC64;
MSGTEEMSKA LKATTPGFCD MPSTIVRASI VQASTVYNDT PKTIEKAGEF IAQAASDGAQ
LVVFPEAYIG GYPRGYRFGI GVGVHNEAGR DCFRRYHASA IVVPGPEVDK LAEMARKNKV
YLVMGAMEKD GYTLYCTALF FSSEGRFLGK HRKVMPTSLE RCIWGYGDGS TIPVYDTPLG
KLGAAICWEN RMPLLRTSLY GKGIELYCAP TADGSTEWQS SMMHIALEGG CFVMSACQFC
LRKDFPEHAD YLFTDWMEDP TIALNMCLST SLKLGLSVRS NPKTLSCSRL SSSVLGSVVL
GLSISSSDHS WLCFPRCLVK SISSCCKLLI ALLTSKILVR FEVLPYTLCK VSHSFFAVSQ
CETRERDAIV SQGGSVIISP LGKILAGPNF ESEGLVTADL DLGDIARAKL YFDVVGHYSR
PDIFNLRVNE NQNKPVTFVS KSVKAADDSE PQDN
//
