ID A0A078G7Q6_BRANA Unreviewed; 621 AA.
AC A0A078G7Q6;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=BnaC03g27480D {ECO:0000313|EMBL:CDY21449.1};
GN ORFNames=DARMORV10_C03P33180.1 {ECO:0000313|EMBL:CAF1701897.1},
GN GSBRNA2T00015850001 {ECO:0000313|EMBL:CDY21449.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY21449.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY21449.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
RN [2] {ECO:0000313|EMBL:CDY21449.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope - CEA;
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAF1701897.1}
RP NUCLEOTIDE SEQUENCE.
RG Genoscope - CEA;
RA William W.;
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; HG994367; CAF1701897.1; -; Genomic_DNA.
DR EMBL; LK032121; CDY21449.1; -; Genomic_DNA.
DR RefSeq; XP_013684034.1; XM_013828580.1.
DR STRING; 3708.A0A078G7Q6; -.
DR PaxDb; 3708-A0A078G7Q6; -.
DR EnsemblPlants; CDY21449; CDY21449; GSBRNA2T00015850001.
DR GeneID; 106388500; -.
DR Gramene; CDY21449; CDY21449; GSBRNA2T00015850001.
DR KEGG; bna:106388500; -.
DR OMA; GNSGMRD; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF36; ENDOGLUCANASE 25; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059}; Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 71..93
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 109..583
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 513
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 561
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 570
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 621 AA; 69199 MW; F75E05E64682B204 CRC64;
MYGRDPWGGP LEIHATDSAT DDDRSRNLND IDRAALSRPL DETQQSWLLG PTEQKKKKYV
DLGCIIVSRK IFVWTVGTIV AAALLAGFIT LIVKTVPRHH QKNPPPDNYT VALHKALKFF
NAQKSGKLPR HNNVSWRGNS GLQDGKGDSG SFYKDLVGGY YDAGDAIKFN FPMAYAMTML
SWSVIEYSAK YEAAGELVHV KELIKWGTDY FLKTFNSTAD SIDDLVSQVG SGNTDDGSTD
PNDHYCWMRP EDMDYKRPVT TCNGGCSDLA AEMAAALASA SIVFKDNREY SKKLVHGAKT
VYQFGRTRRG RYSAGTAESA KFYNSSMYWD EFIWGGAWLY YATGNVTYLD LITKPTMAKH
AGAFWGGPYY GVFSWDNKLA GAQLLLSRLR LFLSPGYPYE EILRTFHNQT SIVMCSYLPY
FNKFNRTRGG LIELNHGDPQ PLQYAANAAF LATLYSDYLD AADTPGWYCG PNFYSTNVLR
EFARTQIDYI LGKNPPKMSY LVGFGTKYPK HVHHRGASIP KNKVKYNCKG GWKWRDSKKP
NPNTIEGAMV AGPDKRDGFR DVRTNYNYTE PTLAGNAGLV AALVALSGEE EASGTIDKNT
IFSAVPPLFP TPPPPPAPWK P
//
