ID A0A078H216_BRANA Unreviewed; 654 AA.
AC A0A078H216;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
GN Name=BnaC09g13230D {ECO:0000313|EMBL:CDY31826.1};
GN ORFNames=GSBRNA2T00050517001 {ECO:0000313|EMBL:CDY31826.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY31826.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY31826.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|ARBA:ARBA00004914}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. Vacuole
CC lumen {ECO:0000256|ARBA:ARBA00004410}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LK032280; CDY31826.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078H216; -.
DR STRING; 3708.A0A078H216; -.
DR PaxDb; 3708-A0A078H216; -.
DR EnsemblPlants; CDY31826; CDY31826; GSBRNA2T00050517001.
DR Gramene; CDY31826; CDY31826; GSBRNA2T00050517001.
DR OMA; TTFFCQD; -.
DR OrthoDB; 315037at2759; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005775; C:vacuolar lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF41; ACID BETA-FRUCTOFURANOSIDASE 3, VACUOLAR; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 38..59
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 16..108
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 116..436
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 439..634
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 654 AA; 73262 MW; E8F299DD3C5C9CEC CRC64;
MASSESLLPV SSQQDPLSDS RRDSIPESRR RRPIKVHLAI YSGFLLIALY VTLVITHGGS
APKNDVNDDT TVESRARLAG VSEKSNDHIW NLSNDLKGEA FAWNNTVLSW QRTAFHFQPE
KNWMNDPNGP LFYKGWYHFF YQYNPNGAVW GDIFWGHAVS KDLIHWLYLP FAMVPDQWYD
ANGVWTGSAT FLDDGSIVML YTGSTDKFVQ VQNLAYPEDP KDPLLLKWTK FSGNPVLVPP
PGIGAKDFRD PTTAWKTSDG KWRITIGSKI NRTGISLIYD TTDFKTYEKH ETLLHQVPNT
GMWECVDFYP VSKTKDKGLD TSVNGPDVKH IIKASMDDTR IDHYAIGTYY DSNATWVPDN
PSIDVGISTG LRYDYGKFYA SKTFYDQHKG RRILWGWIGE SDSEAADVQK GWSSVQGIPR
IVALDTKTGK NLVQWPVKEI ESLRLRSKKF HMKVEPGTVV PVDVGPAAQL DIEAEFKIKK
EDLEVVFADD SVEDDKEFSC ETSGGATVRG ALGPFGFSVL ADQSLSEQTQ VYFYVTKGKD
SKLKTFFCTD TSRSTMANDV VKPVYGSFVP VLKGEKLTMR ILVDHSIIEG FGQGGRTCIT
SRVYPTKAIY GATRLFLFNN ANDAAITASF KVWQMNSAFI HPFSADDLGV PSST
//
