ID A0A078HI08_BRANA Unreviewed; 621 AA.
AC A0A078HI08;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN Name=BnaCnng09110D {ECO:0000313|EMBL:CDY38050.1};
GN ORFNames=GSBRNA2T00065120001 {ECO:0000313|EMBL:CDY38050.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY38050.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY38050.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR EMBL; LK032414; CDY38050.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078HI08; -.
DR STRING; 3708.A0A078HI08; -.
DR PaxDb; 3708-A0A078HI08; -.
DR EnsemblPlants; CDY38050; CDY38050; GSBRNA2T00065120001.
DR Gramene; CDY38050; CDY38050; GSBRNA2T00065120001.
DR OMA; VRFCFES; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999}.
FT DOMAIN 457..503
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 621 AA; 70649 MW; 690C4A19F3AD0148 CRC64;
MDESSQWASA WQLRELFVVL LIYCEVNDPL KLWMHCWKSL GEDILHMQRR RLEFESLNLT
DEEVQQYVLL ELQKLLNDHD KSLADFPDMP LPEKNTLSNV KNSMIQEEKN YNADEENKTH
SELFSKLNSE QLSVYEAVMD SVINCKGKLF FLYGPGGTGK TYVYRTLISK LRSEKRIVLP
VASSGIAATL MPGGRTAHSR FKIPIDIHEN SMCEIKVNTN LAELIQEASL IIWDEAPMAH
RFTFEALDRT LKDIMSNVDP TASDKPFGGK TVLLGGDFRQ VLPVIPQGSR QDTVLASLNR
SFVWDNCNIA ILSKNMRVNQ EEKEFAEWIL KVGNGEAKAA PSSQQEILDL DNIEIDRSLL
LSKREKPLEQ VQQATFPDLQ SSFKDRDYLR VRAILTPRNE TVDEINDFFL SKISGDIKQY
LSADTIANGE NKKNLSSDTN EKGEKDQVGV NLLYTTEYLN ELKCSGLPNH RLDLKNNVPV
ILLRNINQKQ GLCNGTRMMI TRLGERVLEA EILIGKNIGK QILIPRINMT TSDTNCPYQM
RRRQFPVRLC YGMTINKSQG QSLENVGIYL PKPVFSHGQL YVALSRVTSK KGLNIWSEGS
TKGTTTTIKN VVYKEIFNNL R
//
