ID A0A078IAM6_BRANA Unreviewed; 651 AA.
AC A0A078IAM6;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=alanine--tRNA ligase {ECO:0000256|ARBA:ARBA00013168};
DE EC=6.1.1.7 {ECO:0000256|ARBA:ARBA00013168};
GN Name=BnaC05g50520D {ECO:0000313|EMBL:CDY46263.1};
GN ORFNames=GSBRNA2T00083616001 {ECO:0000313|EMBL:CDY46263.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY46263.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY46263.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226}.
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DR EMBL; LK032657; CDY46263.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078IAM6; -.
DR STRING; 3708.A0A078IAM6; -.
DR PaxDb; 3708-A0A078IAM6; -.
DR EnsemblPlants; CDY46263; CDY46263; GSBRNA2T00083616001.
DR Gramene; CDY46263; CDY46263; GSBRNA2T00083616001.
DR OMA; LTCLEIW; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0004813; F:alanine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006419; P:alanyl-tRNA aminoacylation; IBA:GO_Central.
DR Gene3D; 3.10.310.40; -; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR018162; Ala-tRNA-ligase_IIc_anticod-bd.
DR InterPro; IPR018165; Ala-tRNA-synth_IIc_core.
DR InterPro; IPR018164; Ala-tRNA-synth_IIc_N.
DR InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR PANTHER; PTHR11777:SF9; ALANINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR11777; ALANYL-TRNA SYNTHETASE; 1.
DR Pfam; PF01411; tRNA-synt_2c; 2.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF101353; Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS); 1.
DR SUPFAM; SSF55186; ThrRS/AlaRS common domain; 1.
DR PROSITE; PS50860; AA_TRNA_LIGASE_II_ALA; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 1..507
FT /note="Alanyl-transfer RNA synthetases family profile"
FT /evidence="ECO:0000259|PROSITE:PS50860"
SQ SEQUENCE 651 AA; 72486 MW; CC6F87F987AA0AEC CRC64;
MLGNWSFRDY FKKEAIEWAW ELLTKVYELP VDRIYAGLLP DHESRDIWLQ FLPPARVLPF
GCKDNFWEMG DTGPCGPCTE IHYDRVGNRD AASLVNNDDL TCLEIWNLVF IQATGARPYS
GKIGLEDVDR VDMAYRVVAD HIRTLSFAIA DGSRPGNEGR EYVLRRILRR AVRYGKEILK
AEEGFFNGLV SSVIRVMGDV FTELKEHEKK ITEIIKEEEA SFCKTLAKGI EKFRKAGQSV
QGNTLSGEAL FVTLCNVMGV VETDAFILWA TYGFPIDLTQ LMAEEQGLLV DVDGFNKAMK
EARDISKNAQ NKLVAPLSWM LMLSSTLHKM GVSATNDSFK YIWFQVDYER RKLIAPNHTC
THMLNFALKE VLGDHIDQKG SIVLPEKLRY DFSHGTFFSK PVDPEDLRKI ESIVNTQIKD
ELEVYSKESV LSEAKRIKGL RAVFGEVYPD PVRVVAIGRR VEDLLADPEN DEWSSLSSEF
CGEEGIAKGI RRIIAVTTKS AFDALNTASL LEKDVDDTSK AVGNALEKKV NALKRRVDEV
DIPAAKRADI KAKIAQLQLD VGFDAAAVRG AVSKVMQKKG MSVMVFSTDE STNKAVFKQL
DVTEWLTIAL SPIKGKCGKG KSGLASGQGT DASQVSSALD LAASFASMNL N
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