ID A0A081B627_9BURK Unreviewed; 850 AA.
AC A0A081B627;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=AVS7_00130 {ECO:0000313|EMBL:GAD20369.1};
OS Acidovorax sp. MR-S7.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Acidovorax.
OX NCBI_TaxID=1268622 {ECO:0000313|EMBL:GAD20369.1};
RN [1] {ECO:0000313|EMBL:GAD20369.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MR-S7 {ECO:0000313|EMBL:GAD20369.1};
RX DOI=10.1128/genomeA.00412-13;
RA Miura T., Kusada H., Kamagata Y., Hanada S., Kimura N.;
RT "Genome Sequence of the Multiple-beta-Lactam-Antibiotic-Resistant Bacterium
RT Acidovorax sp. Strain MR-S7.";
RL Genome Announc. 1:e00412-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; DF238891; GAD20369.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A081B627; -.
DR STRING; 1268622.AVS7_00130; -.
DR eggNOG; COG0286; Bacteria.
DR eggNOG; COG0732; Bacteria.
DR Proteomes; UP000030646; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.90.220.20; DNA methylase specificity domains; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF4; TYPE I RESTRICTION ENZYME ECOKI METHYLASE SUBUNIT; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF116734; DNA methylase specificity domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:GAD20369.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000030646};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:GAD20369.1}.
FT DOMAIN 186..483
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 522..556
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 850 AA; 94081 MW; 52C32A660880AB66 CRC64;
MAKSATKAIT SGEKKTRAPR KTKAPLTTRE NLSALIGSAR KILRKDKGLN GDVDRLPLLT
WVMFLKFLDD LETVHEEEAE LDGKRYKPII EAPYRWRDWA ARDDGITGDE LLAFISQEAA
IRVDGTVGKG LFAYLRGLAG DGEKGSQREV IANVFKGVQN RMVSGYLLRD IVNKINGIHF
SASEEIHTLS HLYESMLREM RDAAGDSGEF YTPRPVVRFM VQVTDPRLGE TVLDPACGTG
GFLVEAYDHI APQVSTPDQR RVLQQNTLFG QEAKPLPYML VQMNLLLHGL EAPQIAYGNT
LERRVNEIGH SERVDVILTN PPFGGEEEAG IKANFPPNMQ TAETALLFLQ YIMRKLRVAG
APVPGGKPAD RGGRAAVVVP NGTLFGDGIS AVIKEEMLKE FKLHTIVRLP QGVFAPYTDI
PANLLFFERG GPTDTIWYYE LPLPEGRKKY SKTAPLQFEE FAPALEWWND REEGPQAWKV
DFAAKRAAAV EAATPHWQRA ESERNAAIAL GKPIRELEQA VQAAANGQKA ELQDRLKALK
SEQQAHEQAA KSAQAEGDAL YWPIYNLDIK NPSARRSLED VAPSVVIERI FKHEDQIARL
FTSNDINLSP GNEDESNLLR LRSLANKVQV IKASLESIEN DGDLLLAVSF RDAIANAPLR
PMSEVAPLVR REVAIDLEAS YTELGVRSFY KGTFHRRTVA GSEFTWQKMF RVEEGDLIFS
NIMAWEQGIA LAKPEDHGCV GNHRMLTCCA DPAKAVPGFL AYYFMTDEGF AKVYAASPGT
AARNRTLVAA NLEAIEVPVP PLPIQESFNR LQAEVAALKA QHASLRTANA ALLPATLERV
FDGDIPGEQE
//
