UniProt: A0A081LL30

Database: UniProt
Entry: A0A081LL30_AERCA

LinkDB:  A0A081LL30_AERCA 
Original site:  A0A081LL30_AERCA

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Ontology (4)   
   GO (4)   
Chemical reaction (1)   
   KEGG ENZYME (1)   
Gene (1)   
   NCBI-Gene (1)   
Protein sequence (1)   
   RefSeq(pep) (1)   
DNA sequence (13)   
   EMBL (13)   
Protein domain (14)   
   InterPro (7)   
   Pfam (3)   
   PROSITE (2)   
   SMART (2)   
Literature (1)   
   PubMed (1)   
All databases (35)   

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ID   A0A081LL30_AERCA        Unreviewed;       508 AA.
AC   A0A081LL30;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   27-MAR-2024, entry version 61.
DE   RecName: Full=NAD(P) transhydrogenase subunit alpha {ECO:0000256|PIRNR:PIRNR000203};
DE            EC=7.1.1.1 {ECO:0000256|PIRNR:PIRNR000203};
GN   Name=pntA {ECO:0000313|EMBL:BBS15175.1};
GN   ORFNames=C0708_16265 {ECO:0000313|EMBL:AXB09904.1}, C1C91_09880
GN   {ECO:0000313|EMBL:AXB05259.1}, DN604_04015
GN   {ECO:0000313|EMBL:RWT79453.1}, HED45_15840
GN   {ECO:0000313|EMBL:NKD17544.1}, JC965_18820
GN   {ECO:0000313|EMBL:QQA60206.1}, KAM329_042950
GN   {ECO:0000313|EMBL:BCM77749.1}, KAM348_07990
GN   {ECO:0000313|EMBL:GJA53376.1}, KAM351_00120
GN   {ECO:0000313|EMBL:GJA61401.1}, KAM379_11850
GN   {ECO:0000313|EMBL:GJB72127.1}, N5I07_20775
GN   {ECO:0000313|EMBL:MDH1899943.1}, N5I20_05080
GN   {ECO:0000313|EMBL:MDH1504431.1}, N5J40_21270
GN   {ECO:0000313|EMBL:MDH1997227.1}, WP5W18E02_02120
GN   {ECO:0000313|EMBL:BBS15175.1};
OS   Aeromonas caviae (Aeromonas punctata).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=648 {ECO:0000313|EMBL:MDH1504431.1, ECO:0000313|Proteomes:UP001161704};
RN   [1] {ECO:0000313|EMBL:RWT79453.1, ECO:0000313|Proteomes:UP000288891}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GEO_23_Down_B {ECO:0000313|EMBL:RWT79453.1,
RC   ECO:0000313|Proteomes:UP000288891};
RA   Mathys D.A., Mollenkopf D.F., Feicht S.M., Adams R.J., Albers A.L.,
RA   Stuever D.M., Daniels J.B., Wittum T.E.;
RT   "Carbapenemase-producing Enterobacteriaceae present in wastewater treatment
RT   plant effluent and nearby surface waters in the US.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Proteomes:UP000266772, ECO:0000313|Proteomes:UP000266778}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R25-2 {ECO:0000313|Proteomes:UP000266772}, and T25-39
RC   {ECO:0000313|Proteomes:UP000266778};
RA   Shi Y., Tian Z., Leclercq S.O., Zhang H., Yang M., Zhang Y.;
RT   "Genetic characterization and potential molecular dissemination mechanism
RT   of tet (31) gene in Aeromonas caviae from an oxytetracycline wastewater
RT   treatment system.";
RL   J Environ 76:259-266(2019).
RN   [3] {ECO:0000313|EMBL:AXB05259.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=R25-2 {ECO:0000313|EMBL:AXB09904.1}, and T25-39
RC   {ECO:0000313|EMBL:AXB05259.1};
RX   PubMed=30528016; DOI=10.3791/1549;
RA   Shi Y., Tian Z., Leclercq S.O., Zhang H., Yang M., Zhang Y.;
RT   "Genetic characterization and potential molecular dissemination mechanism
RT   of tet (31) gene in Aeromonas caviae from an oxytetracycline wastewater
RT   treatment system.";
RL   J Environ 76:259-266(2019).
RN   [4] {ECO:0000313|EMBL:BBS15175.1, ECO:0000313|Proteomes:UP000515385}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WP5-W18-ESBL-02 {ECO:0000313|EMBL:BBS15175.1,
RC   ECO:0000313|Proteomes:UP000515385};
RA   Sekizuka T., Itokawa K., Yatsu K., Inamine Y., Kuroda M.;
RT   "complete genome sequences of Aeromonas caviae str. WP5-W18-ESBL-02
RT   isolated from wastewater treatment plant effluent.";
RL   Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:NKD17544.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=UFMG-H8 {ECO:0000313|EMBL:NKD17544.1};
RA   Giannattasio-Ferraz S., Maskeri L., Penido A., Barbosa-Stancioli E.F.,
RA   Putonti C.;
RT   "Bacterial samples isolated from urine from healthy bovine heifers (Gyr
RT   breed).";
RL   Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:BCM77749.1, ECO:0000313|Proteomes:UP000594596}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KAM329 {ECO:0000313|EMBL:BCM77749.1,
RC   ECO:0000313|Proteomes:UP000594596};
RA   Suzuki M., Maehana S., Kitasato H.;
RT   "Whole genome sequencing of carbapenem-resistant Aeromonas spp. isolated in
RT   Japan.";
RL   Submitted (OCT-2020) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:QQA60206.1, ECO:0000313|Proteomes:UP000595994}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Aero21 {ECO:0000313|EMBL:QQA60206.1,
RC   ECO:0000313|Proteomes:UP000595994};
RA   Conte D., Mesa D., Palmeiro J.K., Dalla-Costa L.M.;
RT   "GES Beta-lactamases isolated from hospital effluents in Brazil.";
RL   Submitted (DEC-2020) to the EMBL/GenBank/DDBJ databases.
RN   [8] {ECO:0000313|EMBL:GJA53376.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=KAM348 {ECO:0000313|EMBL:GJA53376.1}, KAM351
RC   {ECO:0000313|EMBL:GJA61401.1}, and KAM379
RC   {ECO:0000313|EMBL:GJB72127.1};
RA   Maehana S., Suzuki M., Kitasato H.;
RT   "Draft genome sequence of carbapenem-resistant Aeromonas spp. in Japan.";
RL   Submitted (JUL-2021) to the EMBL/GenBank/DDBJ databases.
RN   [9] {ECO:0000313|EMBL:MDH1504431.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=GD03687 {ECO:0000313|EMBL:MDH1997227.1}, GD03710
RC   {ECO:0000313|EMBL:MDH1504431.1}, and GD03796
RC   {ECO:0000313|EMBL:MDH1899943.1};
RA   Diorio-Toth L.;
RT   "Intensive care unit water sources are persistently colonized with multi-
RT   drug resistant bacteria and are the site of extensive horizontal gene
RT   transfer of antibiotic resistance genes.";
RL   Submitted (SEP-2022) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled to
CC       respiration and ATP hydrolysis and functions as a proton pump across
CC       the membrane. {ECO:0000256|ARBA:ARBA00003943,
CC       ECO:0000256|PIRNR:PIRNR000203}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+);
CC         Xref=Rhea:RHEA:47992, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:57945, ChEBI:CHEBI:58349; EC=7.1.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000006,
CC         ECO:0000256|PIRNR:PIRNR000203};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the AlaDH/PNT family.
CC       {ECO:0000256|ARBA:ARBA00005689, ECO:0000256|PIRNR:PIRNR000203}.
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DR   EMBL; CP025706; AXB05259.1; -; Genomic_DNA.
DR   EMBL; CP025777; AXB09904.1; -; Genomic_DNA.
DR   EMBL; AP022110; BBS15175.1; -; Genomic_DNA.
DR   EMBL; AP024136; BCM77749.1; -; Genomic_DNA.
DR   EMBL; BPNL01000006; GJA53376.1; -; Genomic_DNA.
DR   EMBL; BPNN01000001; GJA61401.1; -; Genomic_DNA.
DR   EMBL; BPOM01000007; GJB72127.1; -; Genomic_DNA.
DR   EMBL; JAOCIZ010000014; MDH1504431.1; -; Genomic_DNA.
DR   EMBL; JAOCFT010000001; MDH1899943.1; -; Genomic_DNA.
DR   EMBL; JAOCJV010000001; MDH1997227.1; -; Genomic_DNA.
DR   EMBL; JAAVMO010000008; NKD17544.1; -; Genomic_DNA.
DR   EMBL; CP065937; QQA60206.1; -; Genomic_DNA.
DR   EMBL; QKPH01000007; RWT79453.1; -; Genomic_DNA.
DR   RefSeq; WP_010675996.1; NZ_WUTZ01000091.1.
DR   GeneID; 70081653; -.
DR   OrthoDB; 9804592at2; -.
DR   Proteomes; UP000266772; Chromosome.
DR   Proteomes; UP000266778; Chromosome.
DR   Proteomes; UP000288891; Unassembled WGS sequence.
DR   Proteomes; UP000515385; Chromosome.
DR   Proteomes; UP000594596; Chromosome.
DR   Proteomes; UP000595994; Chromosome.
DR   Proteomes; UP000705611; Unassembled WGS sequence.
DR   Proteomes; UP000886934; Unassembled WGS sequence.
DR   Proteomes; UP000887009; Unassembled WGS sequence.
DR   Proteomes; UP000887145; Unassembled WGS sequence.
DR   Proteomes; UP001160758; Unassembled WGS sequence.
DR   Proteomes; UP001161297; Unassembled WGS sequence.
DR   Proteomes; UP001161704; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008746; F:NAD(P)+ transhydrogenase activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:1902600; P:proton transmembrane transport; IEA:InterPro.
DR   CDD; cd05304; Rubrum_tdh; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR   InterPro; IPR008143; Ala_DH/PNT_CS2.
DR   InterPro; IPR008142; AlaDH/PNT_CS1.
DR   InterPro; IPR007886; AlaDH/PNT_N.
DR   InterPro; IPR007698; AlaDH/PNT_NAD(H)-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR026255; NADP_transhyd_a.
DR   InterPro; IPR024605; NADP_transhyd_a_C.
DR   NCBIfam; TIGR00561; pntA; 1.
DR   PANTHER; PTHR10160; NAD(P) TRANSHYDROGENASE; 1.
DR   PANTHER; PTHR10160:SF19; PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE; 1.
DR   Pfam; PF01262; AlaDh_PNT_C; 1.
DR   Pfam; PF05222; AlaDh_PNT_N; 1.
DR   Pfam; PF12769; PNTB_4TM; 1.
DR   PIRSF; PIRSF000203; NADP_transhydrogenase_alpha; 1.
DR   SMART; SM01002; AlaDh_PNT_C; 1.
DR   SMART; SM01003; AlaDh_PNT_N; 1.
DR   SUPFAM; SSF52283; Formate/glycerate dehydrogenase catalytic domain-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00836; ALADH_PNT_1; 1.
DR   PROSITE; PS00837; ALADH_PNT_2; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|PIRNR:PIRNR000203};
KW   NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|PIRNR:PIRNR000203};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|PIRNR:PIRNR000203};
KW   Oxidoreductase {ECO:0000313|EMBL:MDH1504431.1};
KW   Translocase {ECO:0000256|ARBA:ARBA00022967, ECO:0000256|PIRNR:PIRNR000203};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        400..419
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        425..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        450..469
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        475..497
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          4..135
FT                   /note="Alanine dehydrogenase/pyridine nucleotide
FT                   transhydrogenase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM01003"
FT   DOMAIN          144..310
FT                   /note="Alanine dehydrogenase/pyridine nucleotide
FT                   transhydrogenase NAD(H)-binding"
FT                   /evidence="ECO:0000259|SMART:SM01002"
SQ   SEQUENCE   508 AA;  53441 MW;  3193F2244791916F CRC64;
     MQIGIPRESL AGETRVAATP ATVEQLKKLG FEVAIEAGAG LRASFDDAAF EAAGASVVPN
     VWQADLVFKV NAPTEAEIAQ IKDGATLVSF IWPAQNPELV KKLSERNINV MAMDMVPRIS
     RAQSLDALSS MANIGGYRAV VEAAHQFGRF FTGQITAAGK VPPAKVLVIG AGVAGLAAIG
     TAGSLGAIVR AFDTRLEVAE QIESMGGEFL KLDFGGEDGA SSDGYAKVMS EEFIKAEMEL
     FAQQAKEVDI IITTALIPGK PAPKLITKEM VDSMKPGSVI VDMAAQAGGN CEYTVPGELH
     VTDNGVKVIG YTDLPGRLPA QSSQLYGTNL VNLMKLMCKE KDGNVAIDFE DVVQRNMTVV
     QAGEVTFPPP AISVSAAPQK PAAAKPAAKK EEAKPSNKKF VFGALGIAAF GWVAAVAPAA
     FLSHFTVFIL ACVVGYYVVW NVSHALHTPL MSVTNAISGI IVVGALLQIG QGSTLVTVLA
     FVAVLIASIN IFGGFTVTQR MLKMFRKD
//

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