ID A0A081LS15_AERCA Unreviewed; 275 AA.
AC A0A081LS15;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 65.
DE RecName: Full=2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase {ECO:0000256|HAMAP-Rule:MF_00811};
DE EC=2.3.1.117 {ECO:0000256|HAMAP-Rule:MF_00811};
DE AltName: Full=Tetrahydrodipicolinate N-succinyltransferase {ECO:0000256|HAMAP-Rule:MF_00811};
DE Short=THDP succinyltransferase {ECO:0000256|HAMAP-Rule:MF_00811};
DE Short=THP succinyltransferase {ECO:0000256|HAMAP-Rule:MF_00811};
DE Short=Tetrahydropicolinate succinylase {ECO:0000256|HAMAP-Rule:MF_00811};
GN Name=dapD {ECO:0000256|HAMAP-Rule:MF_00811,
GN ECO:0000313|EMBL:NKD15655.1};
GN ORFNames=C0708_12475 {ECO:0000313|EMBL:AXB09320.1}, C1C91_13670
GN {ECO:0000313|EMBL:AXB05912.1}, DN604_11375
GN {ECO:0000313|EMBL:RWT75722.1}, HED45_06030
GN {ECO:0000313|EMBL:NKD15655.1}, JC965_08960
GN {ECO:0000313|EMBL:QQA62572.1}, KAM348_26590
GN {ECO:0000313|EMBL:GJA55236.1}, KAM351_23460
GN {ECO:0000313|EMBL:GJA63735.1}, KAM379_08750
GN {ECO:0000313|EMBL:GJB71817.1}, N5I07_16510
GN {ECO:0000313|EMBL:MDH1899134.1}, N5I20_14960
GN {ECO:0000313|EMBL:MDH1506356.1}, N5J40_06095
GN {ECO:0000313|EMBL:MDH1994399.1}, WP5W18E02_10980
GN {ECO:0000313|EMBL:BBS16061.1};
OS Aeromonas caviae (Aeromonas punctata).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=648 {ECO:0000313|EMBL:NKD15655.1, ECO:0000313|Proteomes:UP000705611};
RN [1] {ECO:0000313|EMBL:RWT75722.1, ECO:0000313|Proteomes:UP000288891}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GEO_23_Down_B {ECO:0000313|EMBL:RWT75722.1,
RC ECO:0000313|Proteomes:UP000288891};
RA Mathys D.A., Mollenkopf D.F., Feicht S.M., Adams R.J., Albers A.L.,
RA Stuever D.M., Daniels J.B., Wittum T.E.;
RT "Carbapenemase-producing Enterobacteriaceae present in wastewater treatment
RT plant effluent and nearby surface waters in the US.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000266772, ECO:0000313|Proteomes:UP000266778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R25-2 {ECO:0000313|Proteomes:UP000266772}, and T25-39
RC {ECO:0000313|Proteomes:UP000266778};
RA Shi Y., Tian Z., Leclercq S.O., Zhang H., Yang M., Zhang Y.;
RT "Genetic characterization and potential molecular dissemination mechanism
RT of tet (31) gene in Aeromonas caviae from an oxytetracycline wastewater
RT treatment system.";
RL J Environ 76:259-266(2019).
RN [3] {ECO:0000313|EMBL:AXB05912.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=R25-2 {ECO:0000313|EMBL:AXB09320.1}, and T25-39
RC {ECO:0000313|EMBL:AXB05912.1};
RX PubMed=30528016; DOI=10.3791/1549;
RA Shi Y., Tian Z., Leclercq S.O., Zhang H., Yang M., Zhang Y.;
RT "Genetic characterization and potential molecular dissemination mechanism
RT of tet (31) gene in Aeromonas caviae from an oxytetracycline wastewater
RT treatment system.";
RL J Environ 76:259-266(2019).
RN [4] {ECO:0000313|EMBL:BBS16061.1, ECO:0000313|Proteomes:UP000515385}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WP5-W18-ESBL-02 {ECO:0000313|EMBL:BBS16061.1,
RC ECO:0000313|Proteomes:UP000515385};
RA Sekizuka T., Itokawa K., Yatsu K., Inamine Y., Kuroda M.;
RT "complete genome sequences of Aeromonas caviae str. WP5-W18-ESBL-02
RT isolated from wastewater treatment plant effluent.";
RL Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:NKD15655.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UFMG-H8 {ECO:0000313|EMBL:NKD15655.1};
RA Giannattasio-Ferraz S., Maskeri L., Penido A., Barbosa-Stancioli E.F.,
RA Putonti C.;
RT "Bacterial samples isolated from urine from healthy bovine heifers (Gyr
RT breed).";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:QQA62572.1, ECO:0000313|Proteomes:UP000595994}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Aero21 {ECO:0000313|EMBL:QQA62572.1,
RC ECO:0000313|Proteomes:UP000595994};
RA Conte D., Mesa D., Palmeiro J.K., Dalla-Costa L.M.;
RT "GES Beta-lactamases isolated from hospital effluents in Brazil.";
RL Submitted (DEC-2020) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:GJA55236.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=KAM348 {ECO:0000313|EMBL:GJA55236.1}, KAM351
RC {ECO:0000313|EMBL:GJA63735.1}, and KAM379
RC {ECO:0000313|EMBL:GJB71817.1};
RA Maehana S., Suzuki M., Kitasato H.;
RT "Draft genome sequence of carbapenem-resistant Aeromonas spp. in Japan.";
RL Submitted (JUL-2021) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:MDH1506356.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GD03687 {ECO:0000313|EMBL:MDH1994399.1}, GD03710
RC {ECO:0000313|EMBL:MDH1506356.1}, and GD03796
RC {ECO:0000313|EMBL:MDH1899134.1};
RA Diorio-Toth L.;
RT "Intensive care unit water sources are persistently colonized with multi-
RT drug resistant bacteria and are the site of extensive horizontal gene
RT transfer of antibiotic resistance genes.";
RL Submitted (SEP-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3,4,5-tetrahydrodipicolinate + H2O + succinyl-CoA = (S)-
CC 2-succinylamino-6-oxoheptanedioate + CoA; Xref=Rhea:RHEA:17325,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15685, ChEBI:CHEBI:16845,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57292; EC=2.3.1.117;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00811};
CC -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate
CC (succinylase route): step 1/3. {ECO:0000256|HAMAP-Rule:MF_00811}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000256|HAMAP-Rule:MF_00811}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00811}.
CC -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC {ECO:0000256|ARBA:ARBA00007274, ECO:0000256|HAMAP-Rule:MF_00811}.
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DR EMBL; CP025706; AXB05912.1; -; Genomic_DNA.
DR EMBL; CP025777; AXB09320.1; -; Genomic_DNA.
DR EMBL; AP022110; BBS16061.1; -; Genomic_DNA.
DR EMBL; BPNL01000030; GJA55236.1; -; Genomic_DNA.
DR EMBL; BPNN01000031; GJA63735.1; -; Genomic_DNA.
DR EMBL; BPOM01000005; GJB71817.1; -; Genomic_DNA.
DR EMBL; JAOCIZ010000062; MDH1506356.1; -; Genomic_DNA.
DR EMBL; JAOCFT010000001; MDH1899134.1; -; Genomic_DNA.
DR EMBL; JAOCJV010000001; MDH1994399.1; -; Genomic_DNA.
DR EMBL; JAAVMO010000002; NKD15655.1; -; Genomic_DNA.
DR EMBL; CP065937; QQA62572.1; -; Genomic_DNA.
DR EMBL; QKPH01000033; RWT75722.1; -; Genomic_DNA.
DR RefSeq; WP_010673400.1; NZ_WUTZ01000030.1.
DR GeneID; 70084501; -.
DR KEGG; acav:VI35_15560; -.
DR OrthoDB; 9775362at2; -.
DR UniPathway; UPA00034; UER00019.
DR Proteomes; UP000266772; Chromosome.
DR Proteomes; UP000266778; Chromosome.
DR Proteomes; UP000288891; Unassembled WGS sequence.
DR Proteomes; UP000515385; Chromosome.
DR Proteomes; UP000595994; Chromosome.
DR Proteomes; UP000705611; Unassembled WGS sequence.
DR Proteomes; UP000886934; Unassembled WGS sequence.
DR Proteomes; UP000887009; Unassembled WGS sequence.
DR Proteomes; UP000887145; Unassembled WGS sequence.
DR Proteomes; UP001160758; Unassembled WGS sequence.
DR Proteomes; UP001161297; Unassembled WGS sequence.
DR Proteomes; UP001161704; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008666; F:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:UniProtKB-UniRule.
DR CDD; cd03350; LbH_THP_succinylT; 1.
DR Gene3D; 2.160.10.10; Hexapeptide repeat proteins; 1.
DR Gene3D; 1.10.166.10; Tetrahydrodipicolinate-N-succinyltransferase, N-terminal domain; 1.
DR HAMAP; MF_00811; DapD; 1.
DR InterPro; IPR005664; DapD_Trfase_Hexpep_rpt_fam.
DR InterPro; IPR001451; Hexapep.
DR InterPro; IPR018357; Hexapep_transf_CS.
DR InterPro; IPR023180; THP_succinylTrfase_dom1.
DR InterPro; IPR037133; THP_succinylTrfase_N_sf.
DR InterPro; IPR011004; Trimer_LpxA-like_sf.
DR NCBIfam; TIGR00965; dapD; 1.
DR PANTHER; PTHR19136:SF52; 2,3,4,5-TETRAHYDROPYRIDINE-2,6-DICARBOXYLATE N-SUCCINYLTRANSFERASE; 1.
DR PANTHER; PTHR19136; MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE; 1.
DR Pfam; PF14602; Hexapep_2; 1.
DR Pfam; PF14805; THDPS_N_2; 1.
DR SUPFAM; SSF51161; Trimeric LpxA-like enzymes; 1.
DR PROSITE; PS00101; HEXAPEP_TRANSFERASES; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315, ECO:0000256|HAMAP-
KW Rule:MF_00811};
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605, ECO:0000256|HAMAP-
KW Rule:MF_00811};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00811};
KW Diaminopimelate biosynthesis {ECO:0000256|ARBA:ARBA00022915,
KW ECO:0000256|HAMAP-Rule:MF_00811};
KW Lysine biosynthesis {ECO:0000256|ARBA:ARBA00023154, ECO:0000256|HAMAP-
KW Rule:MF_00811};
KW Repeat {ECO:0000256|ARBA:ARBA00022737, ECO:0000256|HAMAP-Rule:MF_00811};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00811}.
FT DOMAIN 3..69
FT /note="Tetrahydrodipicolinate-N-succinyltransferase chain
FT A"
FT /evidence="ECO:0000259|Pfam:PF14805"
FT BINDING 104
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00811"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00811"
SQ SEQUENCE 275 AA; 29403 MW; 4D6FBB975AD1E12E CRC64;
MTQLQQTIEA AFERRDSITP GSVDAATKAA ILEAIDLLDS GKARVAEKIA GEWVVHQWLK
KAVLLSFRIN DNGIIKGDDA QYYDKVPLKF SNYTEEQFKA AGVRVVPPAT ARKGSFIAPN
TVLMPSYVNI GAFVDEGTMV DTWATVGSCA QIGKNVHLSG GVGIGGVLEP LQANPTIIED
NCFIGARSEV VEGVIVEEGS VISMGVFIGQ STRIYDRETG EIHYGRVPAG SVVVSGSLPS
KCGKYSLYAA VIVKKVDAKT RAKVGINALL RSIDE
//
