ID A0A084A6T8_9GAMM Unreviewed; 1159 AA.
AC A0A084A6T8;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 45.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:KEY61017.1};
GN ORFNames=SRDD_00140 {ECO:0000313|EMBL:KEY61017.1};
OS Serratia sp. DD3.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=1410619 {ECO:0000313|EMBL:KEY61017.1, ECO:0000313|Proteomes:UP000017810};
RN [1] {ECO:0000313|EMBL:KEY61017.1, ECO:0000313|Proteomes:UP000017810}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DD3 {ECO:0000313|EMBL:KEY61017.1,
RC ECO:0000313|Proteomes:UP000017810};
RX PubMed=25212623;
RA Poehlein A., Freese H.M., Daniel R., Simeonova D.D.;
RT "Draft Genome Sequence of Serratia sp. Strain DD3, Isolated from the Guts
RT of Daphnia magna.";
RL Genome Announc. 2:e00903-14(2014).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEY61017.1}.
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DR EMBL; AYKS02000002; KEY61017.1; -; Genomic_DNA.
DR RefSeq; WP_023491792.1; NZ_AYKS02000002.1.
DR AlphaFoldDB; A0A084A6T8; -.
DR STRING; 1410619.SRDD_00140; -.
DR eggNOG; COG1197; Bacteria.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000017810; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000017810}.
FT DOMAIN 625..786
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 808..961
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1159 AA; 131075 MW; FAE455DF6982CEE7 CRC64;
MSTSDNSRRS ALSSARYPLP VRAGDLRQLG QLTGSACAVE CAEITERHSG PVLLIAPDMQ
NALRLRDEIQ QFTDQMVTTL SDWETLPYDS FSPHQEIISS RLSRLYHLPA MESGIIILPV
NTLMQRVCPH EFLRGHALVM KKGQRLSRDL LRTQLEQAGY RCVDQVMEHG EFATRGALLD
LFPMGSDAPF RIDFFDDEID SLRTFDVDSQ RTLSEVESIN LLPAHEFPTD KNAIELFRSQ
WREQFEVRRD AEHIYQQVSK GTWPAGIEYW QPLFFSQPLV SLFSYLPANT LVVNTGDLES
AAERFWQDVN QRYEGRRIDP MRPLLPPETL WLRVDALFGE LKAWPRIALK PEELPDKAGC
CNLGYQPLPD LAVQAQHKSP LDNLRRFIEH FTGSTVFSVE SEGRRETLQE LLGRIKLNPP
VIQRLDELNA TGSYLMVGAA ERGFIDTLRN RAMICESDLL GERVSNRRRD NRRTINTDTL
IRNLAELHPG QPVVHLEHGV GRYVGMTTLE AGGIKAEYLI LAYSGEDKLY VPVSSLHLIN
RYAGGADDNA PLHKLGSDVW SRARQKAAER VRDVAAELLD IYSQRAAKSG FAFKHDREQY
QLFCQTFPFE TTPDQEQAIN AVLSDMCQPL AMDRLVCGDV GFGKTEVAMR AAFLAVENGK
QVAVLVPTTL LAQQHFDNFR DRFANWPFRI EMMSRFRSAK EQQHTLQEAV EGKVDIIIGT
HKLLQSDLRW KDLGLLVVDE EHRFGVRHKE RIKAMRADVD ILTLTATPIP RTLNMAMSGM
RDLSIIATPP ARRLAVKTFV REYDSLVVRE AILREVLRGG QVYYLHNDVD SIDKAAQRLA
ELVPEARIAI GHGQMRERDL ERVMNDFHHQ RFNVLVCTTI IETGIDIPSA NTIVIERADR
FGLAQLHQLR GRVGRSHHQA YAYLLTPNPK VMSTDAHKRL EAIASLEDLG AGFALATHDL
EIRGAGELLG EEQSGQMTTV GFSLYMELLE SAVDALKNGR EPSLEDLTSQ QSEVELRMPT
LLPDDFIPDV NTRLSFYKRI ASAKNEGELE ELKVELIDRF GLLPDAARNL LQSAAMRQQA
LKLGIKRIEG NERGGFIEFG DKNQVDPGYL IGLLQSKPPV YRLDGPTKLK FTLDLSDRLK
RLTFIAELLV AFREHTLAA
//
