ID A0A085EFZ4_9FLAO Unreviewed; 437 AA.
AC A0A085EFZ4;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex {ECO:0000256|RuleBase:RU003423};
DE EC=2.3.1.- {ECO:0000256|RuleBase:RU003423};
GN ORFNames=FEM08_30940 {ECO:0000313|EMBL:KFC58139.1};
OS Flavobacterium gilvum.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1492737 {ECO:0000313|EMBL:KFC58139.1, ECO:0000313|Proteomes:UP000028636};
RN [1] {ECO:0000313|EMBL:KFC58139.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EM1308 {ECO:0000313|EMBL:KFC58139.1};
RA Shin S.-K., Yi H.;
RT "Genome Sequence of Flavobacterium sp. EM1308.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|ARBA:ARBA00001938,
CC ECO:0000256|RuleBase:RU003423};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317, ECO:0000256|RuleBase:RU003423}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFC58139.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JNCP01000115; KFC58139.1; -; Genomic_DNA.
DR RefSeq; WP_035640047.1; NZ_JNCP01000115.1.
DR AlphaFoldDB; A0A085EFZ4; -.
DR STRING; 1492737.EM308_16720; -.
DR KEGG; fgl:EM308_16720; -.
DR PATRIC; fig|1492737.3.peg.3078; -.
DR eggNOG; COG0508; Bacteria.
DR OrthoDB; 9805770at2; -.
DR Proteomes; UP000028636; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd06849; lipoyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 1.
DR InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR43178; DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX; 1.
DR PANTHER; PTHR43178:SF5; LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF02817; E3_binding; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS00189; LIPOYL; 1.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003423};
KW Lipoyl {ECO:0000256|ARBA:ARBA00022823, ECO:0000256|RuleBase:RU003423};
KW Transferase {ECO:0000256|RuleBase:RU003423}.
FT DOMAIN 3..78
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 122..162
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
SQ SEQUENCE 437 AA; 47191 MW; 1B02492E1175BE9F CRC64;
MARFELKLPK MGESVAEATI TNWLKEVGDK IEADDAVLEI ATDKVDSEVP SEVSGILVEK
LFGKDDLVQV GQTIAIIETE AVEAGSPRPE VAPAIVEEIL KTVEVAKETV ATPISFSESD
KFFSPLVKNI AKEEGITLDE LQSIAGSGKE GRVTKDDILD YIKNRSLQPA NAVATPTKEV
ETLKVVSAEA PSQAAAPASV NGADEIVEMD RMRKLISGYM VASIQTSAHV QSFIEVDVTN
IVKWRDKVKN AFEKREGEKL TYTPIFMEAV AKALKDFPGM NISVEGDFII KKKNINIGMA
AALANGNLIV PVIKNADQLN LVGMAKAVND LGNRAKANKL KPDDTQGGTY TVTNVGTFGS
VFGTPIINQP QVGILALGAI RKVPAVIETP DGDFIGIRQK MFLSHSYDHR VVDGALGGSF
VKRVAEYLEA FDVNRDY
//
