ID A0A085G0B4_9ENTR Unreviewed; 707 AA.
AC A0A085G0B4;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN Name=rafA {ECO:0000313|EMBL:KFC77159.1};
GN ORFNames=GBAG_3892 {ECO:0000313|EMBL:KFC77159.1};
OS Buttiauxella agrestis ATCC 33320.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Buttiauxella.
OX NCBI_TaxID=1006004 {ECO:0000313|EMBL:KFC77159.1, ECO:0000313|Proteomes:UP000028653};
RN [1] {ECO:0000313|EMBL:KFC77159.1, ECO:0000313|Proteomes:UP000028653}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33320 {ECO:0000313|EMBL:KFC77159.1,
RC ECO:0000313|Proteomes:UP000028653};
RA Plunkett G.III., Neeno-Eckwall E.C., Glasner J.D., Perna N.T.;
RT "ATOL: Assembling a taxonomically balanced genome-scale reconstruction of
RT the evolutionary history of the Enterobacteriaceae.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFC77159.1}.
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DR EMBL; JMPI01000068; KFC77159.1; -; Genomic_DNA.
DR RefSeq; WP_034499282.1; NZ_JMPI01000068.1.
DR AlphaFoldDB; A0A085G0B4; -.
DR STRING; 1006004.GBAG_3892; -.
DR eggNOG; COG3345; Bacteria.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000028653; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000028653}.
FT DOMAIN 24..236
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 609..693
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 441
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 505
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT BINDING 163
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 329..330
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 406
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 439..443
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 483
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 505
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
SQ SEQUENCE 707 AA; 80269 MW; 10D7CD3C9770A985 CRC64;
MEPIYHRLTS AQSDIVIRTQ PFAEIVYWGQ HLVHFSPLDC ASLDRPIANG RLDIDTPITL
GAELGRGLFG SPGIEGHRNG LDASPIFTTT QVEQNGQNLT ITAEDKQAGL RLISELRLDS
SGVLQIRHGL TNLRATPWQV DRLAITLPIA ERALEVMAFH GRWTREFQPH RIRLEHDSFV
LENRRGRTSH EHFPAMIAGT AAFSEQQGAV WGVHLGWSGN HRLRCEVKTD GRRYLQAEAL
YLAGEMALGE GETLHTPWLY ASHSGNGLNG MSQQFHQFLR EQVIRFPENK PRPVHLNTWE
GIYFDHNPEY IMQMASRAAE LGVERFIIDD GWFKGRNHDR AALGDWYLDE QKYPNGLMPV
INHVKACGME FGIWVEPEMI NPDSDLYRAH PDWVLALPGY EQATGRYQWV LDLSNPQVFD
YLLERMNWLL GEHPIDYVKW DMNRELVQAG HNGKAAADAQ TRQFYRLLDE LNRKYPHVEF
ESCASGGGRI DYEVLKRTHR FWASDNNDAL ERNTIQRGMS YFFPPEVMGA HIGNLRCHAT
YRQHSIEFRG LTALFGHLGM ELDPMTADAA ESAGYERYVQ LYKQWRPLLH TGKQWRVDMP
DATTQVQGVV AEDKSQALYL VSQLAMPDYT LAGNLRVPGL DPAARYCITL LDHPQISVVG
KGGHTMRKLP GWMETTQTVS GEWLAQAGLA LPILDPESAI LIGFERV
//
