ID A0A085G950_9GAMM Unreviewed; 459 AA.
AC A0A085G950;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Isochorismate synthase MenF {ECO:0000256|HAMAP-Rule:MF_01935};
DE EC=5.4.4.2 {ECO:0000256|HAMAP-Rule:MF_01935};
DE AltName: Full=Isochorismate mutase {ECO:0000256|HAMAP-Rule:MF_01935};
GN Name=menF {ECO:0000256|HAMAP-Rule:MF_01935,
GN ECO:0000313|EMBL:KFC80245.1};
GN ORFNames=GEAM_2389 {ECO:0000313|EMBL:KFC80245.1};
OS Ewingella americana ATCC 33852.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Ewingella.
OX NCBI_TaxID=910964 {ECO:0000313|EMBL:KFC80245.1, ECO:0000313|Proteomes:UP000028640};
RN [1] {ECO:0000313|EMBL:KFC80245.1, ECO:0000313|Proteomes:UP000028640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33852 {ECO:0000313|EMBL:KFC80245.1,
RC ECO:0000313|Proteomes:UP000028640};
RA Plunkett G.III., Neeno-Eckwall E.C., Glasner J.D., Perna N.T.;
RT "ATOL: Assembling a taxonomically balanced genome-scale reconstruction of
RT the evolutionary history of the Enterobacteriaceae.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of chorismate to isochorismate.
CC {ECO:0000256|HAMAP-Rule:MF_01935}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chorismate = isochorismate; Xref=Rhea:RHEA:18985,
CC ChEBI:CHEBI:29748, ChEBI:CHEBI:29780; EC=5.4.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000799, ECO:0000256|HAMAP-
CC Rule:MF_01935};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01935};
CC -!- PATHWAY: Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate
CC biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 1/7.
CC {ECO:0000256|HAMAP-Rule:MF_01935}.
CC -!- PATHWAY: Quinol/quinone metabolism; menaquinone biosynthesis.
CC {ECO:0000256|HAMAP-Rule:MF_01935}.
CC -!- SIMILARITY: Belongs to the isochorismate synthase family.
CC {ECO:0000256|ARBA:ARBA00005297, ECO:0000256|HAMAP-Rule:MF_01935}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFC80245.1}.
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DR EMBL; JMPJ01000057; KFC80245.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A085G950; -.
DR STRING; 910964.GEAM_2389; -.
DR eggNOG; COG1169; Bacteria.
DR UniPathway; UPA00079; -.
DR UniPathway; UPA01057; UER00163.
DR Proteomes; UP000028640; Unassembled WGS sequence.
DR GO; GO:0008909; F:isochorismate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009234; P:menaquinone biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.60.120.10; Anthranilate synthase; 1.
DR HAMAP; MF_01935; MenF; 1.
DR InterPro; IPR005801; ADC_synthase.
DR InterPro; IPR015890; Chorismate_C.
DR InterPro; IPR004561; IsoChor_synthase.
DR InterPro; IPR034681; MenF.
DR InterPro; IPR044250; MenF-like.
DR NCBIfam; TIGR00543; isochor_syn; 1.
DR PANTHER; PTHR47253; -; 1.
DR PANTHER; PTHR47253:SF4; ISOCHORISMATE SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00425; Chorismate_bind; 1.
DR SUPFAM; SSF56322; ADC synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01935};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_01935};
KW Menaquinone biosynthesis {ECO:0000256|HAMAP-Rule:MF_01935};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_01935};
KW Reference proteome {ECO:0000313|Proteomes:UP000028640}.
FT DOMAIN 177..428
FT /note="Chorismate-utilising enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00425"
FT REGION 440..459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 198
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01935"
FT ACT_SITE 248
FT /note="Proton donor"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01935"
FT BINDING 292
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01935"
FT BINDING 424
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01935"
SQ SEQUENCE 459 AA; 51101 MW; 6FB57081F8592E67 CRC64;
MAVKQISAIL DQLDTALLGT FPDAAGLRQF TYRLPEQPHG GMLEWLDAQP CYPKFYWQQR
DDAEEVVLCG DAANFSDMPT AQRFLQQYSD FPELRVWGLN AFNDGMGGAQ ASHVLFLPRI
EITRRDGQCN LRCQLYSPVS LREEADKMRA FLRTLVNSRP LPALKSQVVR YQHLPTQPQW
ARMIDAVLSG IKQHQFDKVV LARKTTLALS QPLNPASFLA ASRAVNHHCY HFMLAFAPQQ
AFLGSSPERL FLREKRQLFT EALAGTVAAA DDHQQARALG QWLLNDPKNQ RENMLVVDDI
CQRLQGGARS LDVMPAEILR LRKVQHLRRS IQAVLAKADD ADCLQRLQPT AAVAGLPRQA
AREFIARHEP FDRQWYAGSA GYLSLTRSEF TVALRSAAVK GSQLELFAGA GIVAGSDAAQ
EWLEIENKAA GLRTLLDEGA SSAESAEPQY PELPAVRAG
//
