ID A0A085G9Q2_9GAMM Unreviewed; 196 AA.
AC A0A085G9Q2;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=Probable malonic semialdehyde reductase RutE {ECO:0000256|HAMAP-Rule:MF_01204};
DE EC=1.1.1.298 {ECO:0000256|HAMAP-Rule:MF_01204};
GN Name=rutE {ECO:0000256|HAMAP-Rule:MF_01204};
GN ORFNames=GEAM_2274 {ECO:0000313|EMBL:KFC80447.1};
OS Ewingella americana ATCC 33852.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Ewingella.
OX NCBI_TaxID=910964 {ECO:0000313|EMBL:KFC80447.1, ECO:0000313|Proteomes:UP000028640};
RN [1] {ECO:0000313|EMBL:KFC80447.1, ECO:0000313|Proteomes:UP000028640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33852 {ECO:0000313|EMBL:KFC80447.1,
RC ECO:0000313|Proteomes:UP000028640};
RA Plunkett G.III., Neeno-Eckwall E.C., Glasner J.D., Perna N.T.;
RT "ATOL: Assembling a taxonomically balanced genome-scale reconstruction of
RT the evolutionary history of the Enterobacteriaceae.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May reduce toxic product malonic semialdehyde to 3-
CC hydroxypropionic acid, which is excreted. {ECO:0000256|HAMAP-
CC Rule:MF_01204}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxypropanoate + NADP(+) = 3-oxopropanoate + H(+) +
CC NADPH; Xref=Rhea:RHEA:26438, ChEBI:CHEBI:15378, ChEBI:CHEBI:16510,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.298; Evidence={ECO:0000256|HAMAP-Rule:MF_01204};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01204};
CC -!- SIMILARITY: Belongs to the nitroreductase family. HadB/RutE subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_01204}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFC80447.1}.
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DR EMBL; JMPJ01000054; KFC80447.1; -; Genomic_DNA.
DR RefSeq; WP_034791533.1; NZ_JMPJ01000054.1.
DR AlphaFoldDB; A0A085G9Q2; -.
DR STRING; 910964.GEAM_2274; -.
DR GeneID; 78380612; -.
DR eggNOG; COG0778; Bacteria.
DR OrthoDB; 9784375at2; -.
DR Proteomes; UP000028640; Unassembled WGS sequence.
DR GO; GO:0035527; F:3-hydroxypropionate dehydrogenase (NADP+) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd02148; RutE-like; 1.
DR Gene3D; 3.40.109.10; NADH Oxidase; 1.
DR HAMAP; MF_01204; Oxidoreductase_RutE_HadB; 1.
DR InterPro; IPR029479; Nitroreductase.
DR InterPro; IPR000415; Nitroreductase-like.
DR InterPro; IPR023936; RutE-like.
DR PANTHER; PTHR43543; MALONIC SEMIALDEHYDE REDUCTASE RUTE-RELATED; 1.
DR PANTHER; PTHR43543:SF1; MALONIC SEMIALDEHYDE REDUCTASE RUTE-RELATED; 1.
DR Pfam; PF00881; Nitroreductase; 1.
DR SUPFAM; SSF55469; FMN-dependent nitroreductase-like; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630, ECO:0000256|HAMAP-
KW Rule:MF_01204};
KW FMN {ECO:0000256|ARBA:ARBA00022643, ECO:0000256|HAMAP-Rule:MF_01204};
KW NAD {ECO:0000256|HAMAP-Rule:MF_01204};
KW NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|HAMAP-Rule:MF_01204};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, ECO:0000256|HAMAP-
KW Rule:MF_01204}; Reference proteome {ECO:0000313|Proteomes:UP000028640}.
FT DOMAIN 20..174
FT /note="Nitroreductase"
FT /evidence="ECO:0000259|Pfam:PF00881"
SQ SEQUENCE 196 AA; 21426 MW; DD60DB4AF8C377E8 CRC64;
MAEALTASAL ATLFTEARTH NGWLEKPVSD ELLQQVYELA RMGPTSANCS PARIVFVRSQ
EAKEKLAPAL SSGNLAKTLS APVTAIVGWD PEFYEALPDL FPHGDARSWF TSSPELAHET
AFRNSSLQAA YLIMACRSLG LDTGPMSGFD PAKVDEAFLA EHGWKSNFLI NIGYGDSSKV
YARLPRLDFD RACRLV
//
