ID   A0A085GA67_9GAMM        Unreviewed;       810 AA.
AC   A0A085GA67;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   24-JAN-2024, entry version 30.
DE   SubName: Full=Pyruvate formate-lyase {ECO:0000313|EMBL:KFC80612.1};
DE            EC=2.3.1.54 {ECO:0000313|EMBL:KFC80612.1};
GN   ORFNames=GEAM_1930 {ECO:0000313|EMBL:KFC80612.1};
OS   Ewingella americana ATCC 33852.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Ewingella.
OX   NCBI_TaxID=910964 {ECO:0000313|EMBL:KFC80612.1, ECO:0000313|Proteomes:UP000028640};
RN   [1] {ECO:0000313|EMBL:KFC80612.1, ECO:0000313|Proteomes:UP000028640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33852 {ECO:0000313|EMBL:KFC80612.1,
RC   ECO:0000313|Proteomes:UP000028640};
RA   Plunkett G.III., Neeno-Eckwall E.C., Glasner J.D., Perna N.T.;
RT   "ATOL: Assembling a taxonomically balanced genome-scale reconstruction of
RT   the evolutionary history of the Enterobacteriaceae.";
RL   Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KFC80612.1}.
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DR   EMBL; JMPJ01000053; KFC80612.1; -; Genomic_DNA.
DR   RefSeq; WP_034790945.1; NZ_JMPJ01000053.1.
DR   AlphaFoldDB; A0A085GA67; -.
DR   STRING; 910964.GEAM_1930; -.
DR   GeneID; 78380274; -.
DR   eggNOG; COG1882; Bacteria.
DR   OrthoDB; 9803969at2; -.
DR   Proteomes; UP000028640; Unassembled WGS sequence.
DR   GO; GO:0008861; F:formate C-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   CDD; cd01677; PFL2_DhaB_BssA; 1.
DR   Gene3D; 3.20.70.20; -; 1.
DR   InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR010098; PFL2/GDeHydtase_fam.
DR   InterPro; IPR004184; PFL_dom.
DR   NCBIfam; TIGR01774; PFL2-3; 1.
DR   PANTHER; PTHR43641:SF2; DEHYDRATASE YBIW-RELATED; 1.
DR   PANTHER; PTHR43641; FORMATE ACETYLTRANSFERASE 3-RELATED; 1.
DR   Pfam; PF01228; Gly_radical; 1.
DR   Pfam; PF02901; PFL-like; 1.
DR   PIRSF; PIRSF000379; For_Ac_trans_1; 1.
DR   SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR   PROSITE; PS00850; GLY_RADICAL_1; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
DR   PROSITE; PS51554; PFL; 1.
PE   4: Predicted;
KW   Acyltransferase {ECO:0000313|EMBL:KFC80612.1};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:KFC80612.1};
KW   Organic radical {ECO:0000256|ARBA:ARBA00022818,
KW   ECO:0000256|PIRSR:PIRSR000379-2}; Pyruvate {ECO:0000313|EMBL:KFC80612.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000028640};
KW   Transferase {ECO:0000313|EMBL:KFC80612.1}.
FT   DOMAIN          11..682
FT                   /note="PFL"
FT                   /evidence="ECO:0000259|PROSITE:PS51554"
FT   DOMAIN          689..810
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000259|PROSITE:PS51149"
FT   MOD_RES         786
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000379-2,
FT                   ECO:0000256|PROSITE-ProRule:PRU00493"
SQ   SEQUENCE   810 AA;  90092 MW;  0B69DED09D9A9EF7 CRC64;
     MTQLKLEQLS DRIKAHKEAL IHIVKPPICT ERAQHYTQVY QQHADKPLAV RRALALAEHL
     QKRTIWIKHD ELIVGNQASE VRAAPIFPEY TVGWIESEID ALGDRPGAGF SVSEANKAIL
     HELCPWWRGQ TVQDRCYGMF TDEQKALLDS GIVKAEGNMT SGDAHLAVNF PLLLDLGLDG
     LRNKVAERRE RLQLTDWEDL HKEQLLKAID ITLAAVSEHI LRFAVLAREM AATESREARR
     SELLSMAENC ELIAHQPPQT FWQALQLCYF IQLTLQIESN GHSVSFGRLD QYLYPYFRRD
     VEVAESLSRD AAIELLQSCW LKLLEVNKIR SGTHSKASAG SPLYQNVTIG GQQWRDGQVS
     DAVNPLSYAI LESCGQLRST QPNLSVRYHA GISDDFLDAC VQVIRCGFGM PAFNNDEIVI
     PEFIKLGVEP QDAYDYAAIG CIETAVGGKW GYRCTGMSFI NFARVMLAAL EQGKDAKSGR
     CFLPQQRGLS LGNFDNFEQA FAQWDEQIRY YTRKSIEVEC VVDTVLEENA HDILCSALVD
     DCIERGKTIK QGGAKYDWVS GLQVGIANLG NSLAAVKKLV FEQGQIGQQE LAAALASDYD
     GLDGERLRQR LINGADKYGN DSDEVDDLLV RAYQCYIDEL SNYHNTRFGR GPIGGTYYAG
     TSSISANVPF GAATTATPDG RKAFTPLAEG ASPSSGTDHL GPTAVFNSLG KLPTEAILGG
     VLLNQKLNPA SLENPHDRQT LMLMLRTFFE EHRGWHVQYN IVSRETLLKA KETPQNYRDL
     VVRVAGYSAF FTALSPEAQD DIIARTEHSL
//