ID A0A085JP88_9GAMM Unreviewed; 202 AA.
AC A0A085JP88;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase D {ECO:0000256|ARBA:ARBA00013682, ECO:0000256|PIRNR:PIRNR004553};
DE EC=2.1.1.171 {ECO:0000256|ARBA:ARBA00012141, ECO:0000256|PIRNR:PIRNR004553};
GN ORFNames=GTPT_0458 {ECO:0000313|EMBL:KFD22284.1};
OS Tatumella ptyseos ATCC 33301.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Tatumella.
OX NCBI_TaxID=1005995 {ECO:0000313|EMBL:KFD22284.1, ECO:0000313|Proteomes:UP000028602};
RN [1] {ECO:0000313|EMBL:KFD22284.1, ECO:0000313|Proteomes:UP000028602}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33301 {ECO:0000313|EMBL:KFD22284.1,
RC ECO:0000313|Proteomes:UP000028602};
RA Plunkett G.III., Neeno-Eckwall E.C., Glasner J.D., Perna N.T.;
RT "ATOL: Assembling a taxonomically balanced genome-scale reconstruction of
RT the evolutionary history of the Enterobacteriaceae.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Specifically methylates the guanine in position 966 of 16S
CC rRNA in the assembled 30S particle. {ECO:0000256|ARBA:ARBA00002649,
CC ECO:0000256|PIRNR:PIRNR004553}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(966) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(2)-methylguanosine(966) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:23548, Rhea:RHEA-COMP:10211, Rhea:RHEA-COMP:10212,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.171;
CC Evidence={ECO:0000256|ARBA:ARBA00000252,
CC ECO:0000256|PIRNR:PIRNR004553};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmD family.
CC {ECO:0000256|ARBA:ARBA00005269, ECO:0000256|PIRNR:PIRNR004553}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFD22284.1}.
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DR EMBL; JMPR01000008; KFD22284.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A085JP88; -.
DR eggNOG; COG0742; Bacteria.
DR Proteomes; UP000028602; Unassembled WGS sequence.
DR GO; GO:0052913; F:16S rRNA (guanine(966)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004398; RNA_MeTrfase_RsmD.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00095; 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD; 1.
DR PANTHER; PTHR43542; METHYLTRANSFERASE; 1.
DR PANTHER; PTHR43542:SF1; METHYLTRANSFERASE; 1.
DR Pfam; PF03602; Cons_hypoth95; 1.
DR PIRSF; PIRSF004553; CHP00095; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|PIRNR:PIRNR004553,
KW ECO:0000313|EMBL:KFD22284.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000028602};
KW rRNA processing {ECO:0000256|PIRNR:PIRNR004553};
KW S-adenosyl-L-methionine {ECO:0000256|PIRNR:PIRNR004553};
KW Transferase {ECO:0000256|PIRNR:PIRNR004553, ECO:0000313|EMBL:KFD22284.1}.
SQ SEQUENCE 202 AA; 22386 MW; 32BB9E92FA47B299 CRC64;
MMNMNKHSRG PAGAGQVRII GGQWRGRKLP VPVSAGLRPT TDRVRETLFN WLAPEMAQAR
CLDCFAGSGA LGIEALSRYA GHVTLLELDP AVARQLSANL QTLKTTQGHV VQGNTLQWLN
TPGEPYDIVF VDPPFRKGLL SETLTLLENK GWLSGEALIY IECETEHAPP EVPASWRLYR
EKVAGQVAYR LYHRSVPDRQ GE
//
