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Database: UniProt
Entry: A0A085VEQ9_PSESX
LinkDB: A0A085VEQ9_PSESX
Original site: A0A085VEQ9_PSESX 
ID   A0A085VEQ9_PSESX        Unreviewed;       438 AA.
AC   A0A085VEQ9;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   18-SEP-2019, entry version 24.
DE   RecName: Full=GDP-mannose 6-dehydrogenase {ECO:0000256|PIRNR:PIRNR000124};
DE            EC=1.1.1.132 {ECO:0000256|PIRNR:PIRNR000124};
GN   ORFNames=IV01_17935 {ECO:0000313|EMBL:KFE53922.1};
OS   Pseudomonas syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=317 {ECO:0000313|EMBL:KFE53922.1, ECO:0000313|Proteomes:UP000028631};
RN   [1] {ECO:0000313|EMBL:KFE53922.1, ECO:0000313|Proteomes:UP000028631}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GAW0119 {ECO:0000313|EMBL:KFE53922.1,
RC   ECO:0000313|Proteomes:UP000028631};
RA   Baltrus D.A., Berge O., Morris C.;
RT   "Draft Genome Sequences of Environmental Pseudomonas syringae
RT   strains.";
RL   Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GDP-alpha-D-mannose + H2O + 2 NAD(+) = GDP-alpha-D-
CC         mannuronate + 3 H(+) + 2 NADH; Xref=Rhea:RHEA:21728,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:84886;
CC         EC=1.1.1.132; Evidence={ECO:0000256|PIRNR:PIRNR000124};
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC       {ECO:0000256|PIRNR:PIRNR000124}.
CC   -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC       family. {ECO:0000256|PIRNR:PIRNR000124}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KFE53922.1}.
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DR   EMBL; JPQU01000050; KFE53922.1; -; Genomic_DNA.
DR   RefSeq; WP_032630087.1; NZ_JPQU01000050.1.
DR   EnsemblBacteria; KFE53922; KFE53922; IV01_17935.
DR   PATRIC; fig|317.175.peg.3740; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000028631; Unassembled WGS sequence.
DR   GO; GO:0047919; F:GDP-mannose 6-dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR028358; GDPman_DH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR017476; UDP-Glc/GDP-Man.
DR   InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR   InterPro; IPR036220; UDP-Glc/GDP-Man_DH_C_sf.
DR   InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR   InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR   Pfam; PF00984; UDPG_MGDP_dh; 1.
DR   Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR   Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR   PIRSF; PIRSF500135; GDPman_DH; 1.
DR   PIRSF; PIRSF000124; UDPglc_GDPman_dh; 1.
DR   SMART; SM00984; UDPG_MGDP_dh_C; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52413; SSF52413; 1.
DR   TIGRFAMs; TIGR03026; NDP-sugDHase; 1.
PE   3: Inferred from homology;
KW   Alginate biosynthesis {ECO:0000256|PIRNR:PIRNR000124};
KW   Complete proteome {ECO:0000313|Proteomes:UP000028631};
KW   NAD {ECO:0000256|PIRNR:PIRNR000124, ECO:0000256|PIRSR:PIRSR500135-3};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000124};
KW   Reference proteome {ECO:0000313|Proteomes:UP000028631}.
FT   DOMAIN      317    425       UDPG_MGDP_dh_C. {ECO:0000259|SMART:
FT                                SM00984}.
FT   NP_BIND       7     12       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   REGION      157    161       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR500135-2}.
FT   REGION      210    217       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR500135-2}.
FT   REGION      256    265       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR500135-2}.
FT   ACT_SITE    268    268       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR500135-1}.
FT   BINDING      30     30       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   BINDING      35     35       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   BINDING      86     86       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   BINDING     105    105       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   BINDING     124    124       NAD; via amide nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   BINDING     271    271       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
FT   BINDING     331    331       NAD. {ECO:0000256|PIRSR:PIRSR500135-3}.
SQ   SEQUENCE   438 AA;  47509 MW;  F1B13EDC94F000F7 CRC64;
     MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSAKIDLIN SGKSPIVEPG LGELLDRGIR
     TGKLRGTTDF SEAIRATDVS MICVGTPSKK NGDLELDYIE AVCREIGFVL RDKASRHTIV
     VRSTVLPGTV ANVVIPILED CSGKKAGVDF GVAVNPEFLR ESTAIKDYDH PPMTVIGEFD
     KASGDVLQAL YEELDAPIIR KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKACGVDGRE
     VMEVVCQDKQ LNLSQYYMRP GFAFGGSCLP KDVRALTYRA GSLDVEAPLL NSLMRSNVSQ
     VQNAFDIVAA HDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFELS IFDSNVEYAR
     VHGSNKEYIE SKIPHVSSLL NSDFDKVIND SDVIILGNRD ERFRALADKA PAGKHVVDLV
     GFMTTTTSEN ERAEGICW
//
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