ID A0A085Z1F6_9FLAO Unreviewed; 525 AA.
AC A0A085Z1F6;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=malate synthase {ECO:0000256|ARBA:ARBA00012636};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636};
GN ORFNames=IX38_19305 {ECO:0000313|EMBL:KFE98269.1};
OS Chryseobacterium luteum.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Chryseobacterium.
OX NCBI_TaxID=421531 {ECO:0000313|EMBL:KFE98269.1, ECO:0000313|Proteomes:UP000028703};
RN [1] {ECO:0000313|EMBL:KFE98269.1, ECO:0000313|Proteomes:UP000028703}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18605 {ECO:0000313|EMBL:KFE98269.1,
RC ECO:0000313|Proteomes:UP000028703};
RA Stropko S.J., Pipes S.E., Newman J.D.;
RT "Genome of Chryseobacterium luteum DSM 18605.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699};
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFE98269.1}.
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DR EMBL; JPRO01000022; KFE98269.1; -; Genomic_DNA.
DR RefSeq; WP_034707381.1; NZ_JPRO01000022.1.
DR AlphaFoldDB; A0A085Z1F6; -.
DR STRING; 421531.IX38_19305; -.
DR eggNOG; COG2225; Bacteria.
DR OrthoDB; 9768429at2; -.
DR Proteomes; UP000028703; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 12..65
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 157..399
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 404..521
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 160
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 439
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 525 AA; 60177 MW; 5CD2E71BFE77EEBF CRC64;
METSIQLKIK AHKQFGEVFT PELTDFLVAL HQNFNQKRRS LLEERKKTQQ LFDLGNLPGF
LPETEEIRNG NWTCSPLPPD LLDRRVEITG PVDRKMIINA LNSVASAFMA DFEDSSSPTW
ENCIQGQINL ADAVRRTIDF TAENGKSYQL HEKTAVLQVR PRGLHLPEKH IEINGEEASG
SLTDFGIYFF SNAEQLLKNG SGPYFYLPKL EHYKEARWWN DVFIFAQNYL GIPEGTVKAT
VLIETITASF QIDEILFELK HHSAGLNCGR WDYIFSFIKK FRNLPGFMVP DRDQVTMTSP
FMSAYSKRVI EICHKRNVHA IGGMAAQIPV KNDDKANEAA FEKVRKDKER EAKNGHDGTW
VAHPALVSIA KEIFDRYMPS QNQIGKKLEY HIKEADLLEV PQGQITEKGV RKNINVGILY
LESWLMGVGA AAIYNLMEDA ATAEISRTQL WQWLKNDAKL DSGLTLTREI ILQWEAEEIE
NIKKYVGEQR FKNGKFNLAK ELFNELVFSE KFEEFLTLKA YPFIE
//
