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Database: UniProt
Entry: A0A087BSE0_9BIFI
LinkDB: A0A087BSE0_9BIFI
Original site: A0A087BSE0_9BIFI 
ID   A0A087BSE0_9BIFI        Unreviewed;       804 AA.
AC   A0A087BSE0;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   11-DEC-2019, entry version 26.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974};
GN   ORFNames=BMON_1609 {ECO:0000313|EMBL:KFI73940.1};
OS   Bifidobacterium mongoliense DSM 21395.
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=1437603 {ECO:0000313|EMBL:KFI73940.1, ECO:0000313|Proteomes:UP000029082};
RN   [1] {ECO:0000313|EMBL:KFI73940.1, ECO:0000313|Proteomes:UP000029082}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21395 {ECO:0000313|EMBL:KFI73940.1,
RC   ECO:0000313|Proteomes:UP000029082};
RA   Ventura M., Milani C., Lugli G.A.;
RT   "Genomics of Bifidobacteria.";
RL   Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA replication.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00974};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-Rule:MF_00974};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC       domain that contains the primase activity, and a C-terminal DnaB-
CC       binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-
CC       Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KFI73940.1}.
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DR   EMBL; JGZE01000030; KFI73940.1; -; Genomic_DNA.
DR   STRING; 1437603.BMON_1609; -.
DR   EnsemblBacteria; KFI73940; KFI73940; BMON_1609.
DR   Proteomes; UP000029082; Unassembled WGS sequence.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR019475; DNA_primase_DnaB-bd.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF10410; DnaB_bind; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
PE   3: Inferred from homology;
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709327}; Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709339};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000029082};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993442};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN          37..91
FT                   /note="ZnF_CHCC"
FT                   /evidence="ECO:0000259|SMART:SM00400"
FT   DOMAIN          262..355
FT                   /note="Toprim"
FT                   /evidence="ECO:0000259|SMART:SM00493"
FT   ZN_FING         41..65
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00974"
FT   REGION          470..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          779..804
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        779..795
FT                   /note="Polar"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   804 AA;  89144 MW;  D5EA3EC54DB0B36D CRC64;
     MAGMILKEDV EKVRASADLY DIVSATVTLK PSGSGTYVGL CPFHDEKTPS FSVRPSLGVW
     HCFGCGLGGD VFGYVEHQEN IDFREAVELL ADRYHIDLRY EEGSARSEHT GSKRSRLLEA
     NEEAQRFFTA QIVSKEALAA RKLLGGRNFS QAECERFGCG YAPQGWDNLV RHLSGKGFTQ
     QEMLDAGLAR QGQRGIYDYF RGRATWPIRD STGRTLGFGA RKLYDDDSIG AKYINTPDTQ
     LYRKTQALYG IDLAKKAIVA KRQAVIVEGY TDVMACHLAG IDMAVATCGT AFGAEHAKIV
     RRLISDDSLG AVQLIGPLKV EGQPLSSRIV FTFDGDAAGQ KAALHAFSLD SAFLSQTFVA
     VADGNLDPCD LRIQQGDEAV RSLIAGAKPL YDFVIDTAIG RFDTAFTTGQ MGAVKAVAPL
     IAQIRDRSLL DIYTRKATRR IGVELDLMRR EVSTARRQQH IRDEDAYAPR RRFPQGERQG
     YGERDRDGGV YENPARRKTL ERRDMNEQTY YRIDDAVFIC EQQFMAMLIQ MPLAVDREWF
     TRLTLNNFMT PVFRSLFQAV SAAGGLPDEN TPQGLWLHAL SKAGGPCSNR SSTSSPSCRC
     RCRPRITAAR TMAAPRRPAT VRAHRGCTVP APPKASMPHS CWCGCTTSGS CVASAPHADV
     WRSCRMVRRR SICSARSRSW RPPARTCRIR CTATPPRRSS EVVRRPYNRS LVIRRRRWRR
     SYGGSHTVPA MARTARDDRR RGAVQCPAAM LWDETDDGGA GRLVCDGDFV CSAPITPFSS
     SRRYSPSSHV SSFPPTPGTP DMCM
//
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