ID A0A087DE22_9BIFI Unreviewed; 1403 AA.
AC A0A087DE22;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BSTEL_2049 {ECO:0000313|EMBL:KFI93772.1};
OS Bifidobacterium stellenboschense.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=762211 {ECO:0000313|EMBL:KFI93772.1, ECO:0000313|Proteomes:UP000029004};
RN [1] {ECO:0000313|EMBL:KFI93772.1, ECO:0000313|Proteomes:UP000029004}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 23968 {ECO:0000313|EMBL:KFI93772.1,
RC ECO:0000313|Proteomes:UP000029004};
RA Ventura M., Milani C., Lugli G.A.;
RT "Genomics of Bifidobacteria.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFI93772.1}.
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DR EMBL; JGZP01000026; KFI93772.1; -; Genomic_DNA.
DR RefSeq; WP_034530179.1; NZ_JGZP01000026.1.
DR STRING; 762211.BSTEL_2049; -.
DR eggNOG; COG0210; Bacteria.
DR eggNOG; COG2887; Bacteria.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000029004; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000029004}.
FT DOMAIN 8..396
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 397..746
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 948..994
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1059..1091
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1227..1255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1059..1081
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1239..1255
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 29..36
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1403 AA; 151537 MW; D3A8A1B1EAEB189E CRC64;
MSSTPKFTDS PEQAAVINAP ATDDVLVVAG AGSGKTYTMT RRIIELIRRG VAPERILGLT
FTRKAAGELL SRVSAAVSAD DADGNAPNGS GAEGTAEQAE AIRAAGDRMF LKPVVYTYDA
FFQSIVRQYG LLVGFDQNTQ PLSEAGALQL AANVIDRNMD RLRGHDLGAF NTLAGKVLAL
SGAIGSAMIG HGVADMDAAI ERVRAWDGAF AAQVRDAIGS EPIPEEPPKL TKPRRLKKDS
DAAWTAKLDE YHERLHAACV FHCAELADTA EQRDTLLDLV EAYTREKRRL NLAEFGDFTI
AAYQLVTRFP SIGERYRQRF SHVLLDEYQD TSTTQAALLA TLFHDGGTAV NAVGDPFQSI
YAWRGASPGA FRLFQQDFGM QENSRPYELS VTRRNSRLVL EAANDLTLPL RETPRRAGSS
TMREVDVAPL DTLPDASTGT IGVLGYDTLG QEIDGVVRFC RHALAVNDHG EPDKPPTTAV
LFRSKKHMAA YQQALEQAGL STLVVGYSAL LDRPEIRDVL ALLHVASDHT DSGSLMRLLA
TPRFGLDAAT LAAFAALADA ANTEVRYQAL VAAGLAPADA PCRDRAGIVR EHRDSVANAV
FLPDLLLDPD LPGMLERGGR FDADSAREIM HASRMLHMVR GMVGRPLPDV VRTAVQALDL
DIDTVVAQAM RNHATAPDPT VARIPMETII DLVDTYTSEI AAESTPTLRG FMAWVDALRT
VEDETAALDS DRHVDVELMT VHQSKGLEWD AVAVVGLAEG AFPSSQGDHL AVTLDDAHPG
GVENGVWTAP EYHERASTWL DDPTAVPVPV RADAGILQRF PHDASTDADP LDTLRALDDV
ELIDDEVYGS LRAVGDGVND VDPDGWYLTQ EEEYGRRLHA DERRLMYVAL TRARRDALLT
YSRNGETGRD PSVLGEKPRM AKPSNFWLEV HDALHAHDGT VFAATPASVG ADTGETDRET
ETEAAHGAPV LSNGSDGTGE PPRTPANTGA PRPDGFFTGE HAAGYAHAVI DEAWEAPLEE
RTESALPWPS ALGGVTASSL LAAARAVRAA MYARTLTDNT DDGAQSFETP GGSSPDGSSS
GGPSPDGPAR GTLLDKARLL IADDDLMSGV LDDASLDERV KSQAQRILAG SRQSVTRLQA
RAADMGEREE RRYWRGIVRP IPSIASPAAS AGTRFHAWAE HFIDAAIDTG IEAGSSTGTG
IDTETGAIMD RHAMLADLER REAAIAAEGS GASGRNDASD KPGAEDDGRN TPRRIPRVER
ELVAWQRRLA ESPWARRTPV WAERQLVVAL PELDGIIANG KLDAVFLGGL DPDDATKRYT
VIDWKTGRRP RKPKDMADKL VQLDLYRLLL AAMENVPLDS IDAALYYVSE PDTELRELRA
EEKTEQEIIA ELSSGIPEHT DDD
//
