ID A0A087H1Q3_ARAAL Unreviewed; 629 AA.
AC A0A087H1Q3;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN OrderedLocusNames=AALP_Aa4g071900 {ECO:0000313|EMBL:KFK36055.1};
OS Arabis alpina (Alpine rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis.
OX NCBI_TaxID=50452 {ECO:0000313|EMBL:KFK36055.1, ECO:0000313|Proteomes:UP000029120};
RN [1] {ECO:0000313|Proteomes:UP000029120}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pajares {ECO:0000313|Proteomes:UP000029120};
RX PubMed=27246759; DOI=10.1038/nplants.2014.23;
RA Willing E.M., Rawat V., Mandakova T., Maumus F., James G.V.,
RA Nordstroem K.J., Becker C., Warthmann N., Chica C., Szarzynska B.,
RA Zytnicki M., Albani M.C., Kiefer C., Bergonzi S., Castaings L.,
RA Mateos J.L., Berns M.C., Bujdoso N., Piofczyk T., de Lorenzo L.,
RA Barrero-Sicilia C., Mateos I., Piednoel M., Hagmann J., Chen-Min-Tao R.,
RA Iglesias-Fernandez R., Schuster S.C., Alonso-Blanco C., Roudier F.,
RA Carbonero P., Paz-Ares J., Davis S.J., Pecinka A., Quesneville H.,
RA Colot V., Lysak M.A., Weigel D., Coupland G., Schneeberger K.;
RT "Genome expansion of Arabis alpina linked with retrotransposition and
RT reduced symmetric DNA methylation.";
RL Nat. Plants 1:14023-14023(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM002872; KFK36055.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A087H1Q3; -.
DR EnsemblPlants; KFK36055; KFK36055; AALP_AA4G071900.
DR Gramene; KFK36055; KFK36055; AALP_AA4G071900.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR OMA; GFDKYYN; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000029120; Chromosome 4.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF41; ENDOGLUCANASE 5; 1.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000029120};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 25..629
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5005106548"
FT DOMAIN 537..617
FT /note="Carbohydrate binding"
FT /evidence="ECO:0000259|SMART:SM01063"
FT ACT_SITE 416
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 629 AA; 69528 MW; BED20935E87A0CC2 CRC64;
MGKFGGSLFR VSLLLTVILG AATAAAEYYN YANALDKTFL FFEAQRSGKL PAAQRVKWRS
HSGLKDGLAQ GVSLEGGYYD AGDHVKFGLP MAFAVTMLSW AAVDNRKELS SSNQMQQTLW
SIRWGTDYFI KAHPQPNVLW GQVGDGESDH YCWERAEDMT TSRTAYKLDQ YHPGSDLAGE
TAAALAAASL AFKPFNSSYS SLLLTHAKEL FSFADKYRGL YTDSIPNAKP FYMSSGYSDE
LLWAAAWLHR ATGDQYYLKY VVDNAGYMGG TGWGMKEFSW DNKYAGVQIL LSKVLLEGKG
GAYTSTLKQY QMKGDYFACA CLKKNGGYNI QTTPGGLMYV REWNNLQYAS AAAFLLAVYS
DYLSAANAKL NCPDGSVQPQ ALLDFATSQA DYILGKNRQG MSYLVGYGPK YPIRVHHRGA
SIASIFTQRS SVNCVQGFDS WYKRSQADPN VIYGALVGGP DQNDYYSDER SNYEQSEPTL
SGTAPLVGLF AKLSGKSGSY GGGGSYKPYQ QTKPPASSSY YKATQSTYTP KQSEAAIEFL
HSITTNWMAG NKRYYRHKVI IKNNSQKPIS DLKLKIEDLS GPIWGLDTTG QKNTYQLPQW
QKVLKAGQAY DFVYVQGGPQ AKVTVLSYH
//
