ID A0A087HD14_ARAAL Unreviewed; 1198 AA.
AC A0A087HD14;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=RNA-dependent RNA polymerase {ECO:0000256|RuleBase:RU363098};
DE EC=2.7.7.48 {ECO:0000256|RuleBase:RU363098};
GN OrderedLocusNames=AALP_Aa3g319000 {ECO:0000313|EMBL:KFK40016.1};
OS Arabis alpina (Alpine rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis.
OX NCBI_TaxID=50452 {ECO:0000313|EMBL:KFK40016.1, ECO:0000313|Proteomes:UP000029120};
RN [1] {ECO:0000313|Proteomes:UP000029120}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pajares {ECO:0000313|Proteomes:UP000029120};
RX PubMed=27246759; DOI=10.1038/nplants.2014.23;
RA Willing E.M., Rawat V., Mandakova T., Maumus F., James G.V.,
RA Nordstroem K.J., Becker C., Warthmann N., Chica C., Szarzynska B.,
RA Zytnicki M., Albani M.C., Kiefer C., Bergonzi S., Castaings L.,
RA Mateos J.L., Berns M.C., Bujdoso N., Piofczyk T., de Lorenzo L.,
RA Barrero-Sicilia C., Mateos I., Piednoel M., Hagmann J., Chen-Min-Tao R.,
RA Iglesias-Fernandez R., Schuster S.C., Alonso-Blanco C., Roudier F.,
RA Carbonero P., Paz-Ares J., Davis S.J., Pecinka A., Quesneville H.,
RA Colot V., Lysak M.A., Weigel D., Coupland G., Schneeberger K.;
RT "Genome expansion of Arabis alpina linked with retrotransposition and
RT reduced symmetric DNA methylation.";
RL Nat. Plants 1:14023-14023(2015).
CC -!- FUNCTION: Probably involved in the RNA silencing pathway and required
CC for the generation of small interfering RNAs (siRNAs).
CC {ECO:0000256|RuleBase:RU363098}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|ARBA:ARBA00024517,
CC ECO:0000256|RuleBase:RU363098};
CC -!- SIMILARITY: Belongs to the RdRP family. {ECO:0000256|ARBA:ARBA00005762,
CC ECO:0000256|RuleBase:RU363098}.
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DR EMBL; CM002871; KFK40016.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A087HD14; -.
DR EnsemblPlants; KFK40016; KFK40016; AALP_AA3G319000.
DR Gramene; KFK40016; KFK40016; AALP_AA3G319000.
DR eggNOG; KOG0988; Eukaryota.
DR OMA; HPKWVKK; -.
DR OrthoDB; 5477883at2759; -.
DR Proteomes; UP000029120; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IEA:EnsemblPlants.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0048467; P:gynoecium development; IEA:EnsemblPlants.
DR GO; GO:0048366; P:leaf development; IEA:EnsemblPlants.
DR GO; GO:0048544; P:recognition of pollen; IEA:EnsemblPlants.
DR GO; GO:0009616; P:RNAi-mediated antiviral immune response; IEA:EnsemblPlants.
DR GO; GO:0010267; P:ta-siRNA processing; IEA:EnsemblPlants.
DR CDD; cd00590; RRM_SF; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR007855; RNA-dep_RNA_pol_euk-typ.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR23079; RNA-DEPENDENT RNA POLYMERASE; 1.
DR PANTHER; PTHR23079:SF18; RNA-DEPENDENT RNA POLYMERASE 6; 1.
DR Pfam; PF05183; RdRP; 1.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR PROSITE; PS50102; RRM; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Nucleotidyltransferase {ECO:0000256|RuleBase:RU363098};
KW Reference proteome {ECO:0000313|Proteomes:UP000029120};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00176};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU363098};
KW RNA-mediated gene silencing {ECO:0000256|RuleBase:RU363098};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363098}.
FT DOMAIN 13..115
FT /note="RRM"
FT /evidence="ECO:0000259|PROSITE:PS50102"
FT COILED 1084..1123
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1198 AA; 137027 MW; C8D640E5B84A6CA3 CRC64;
MGSEGNNKNS VVTQVSIGGF WESTTAKELT DYLEDEVGLV WRCRLKTSWT PLWSYPNFEI
TDTSDIPTID DYKKVEPHAF IHFAVPDSAA RAMDAAGQSK LILDGQPLKV SLGPKNPYTL
NQRRRTTTPF KLAGISIEIG TLVSRDEFLV SWRAEGVDFL VDPFDNTCRL CFQKSTAFAF
KDTVMHAVIN CDYKLEFLVR DIRSVRQYRK LDQFVVLLHL ASSPRVWYRT ADDDICETIP
ADLLDDDDPW IRTTDFTQTG AIGRCLSYRV LISPRYEKKL TATLEYLRMR RVEEKRVKWP
PRIRDEPCFG DPVADHFFCI HHKEGISFEI MFLVNSVLHK GIFNQFQLTE SFFGLLRNQP
RDVNLASLKH LCTYKRPVFD AYKRLKLVQE WIQKNPKLLG SHEPSDDISE IRRLAITPTR
AYCLPPEVEL SNRVLRKYKA VSDRFLRVTF MDESMQTMNS NVLSYFVAPI VKDLTSSSYS
QKTLVFKRVK TILTEGFKLC GRKYSFLAFS SNQLRDRSAW FFAEDGKTRV SDIKKWMGKF
KDKNVAKCAA RMGLCFSSTY ASVDVMYHEV DTELPEIERN GYVFSDGIGT ITPDLAVEVT
EKLKLDVNCT PCAYQIRYAG FKGVVARWPS KGDGIRLALR PSMNKFHSNH TILEICSWTR
FQPGFLNRQI ITLLSVLGVP DEVFWDMQET MLGKLNRILD DIDVAFEVLT ASCAEQGNTA
AIMLSAGFKP KTEPHLRGML SSVRIAQLWG LREKSRIFVP PGRWLMGCLD EAAILEQGQC
FIQVSKPSIE NCFSKHGSRF KETKADLQVV KGYVAIAKNP CLHPGDVRIL EAVDVPQLHH
MYDCLIFPQK GDRPHPDEAS GSDLDGDLYF VAWDQQLIPP SRKSWPAMQY DAAEAKTLGR
AVNHQDIIDF FVQNMANEHL GTICNAHVVH ADRSDYGAMD EECLLLAELA AKAVDFPKTG
KMVTMPFHLK PKLYPDFMGK EDYQTYKSTK ILGRLYRRVK EVYDEDAEAS SEENSDPSDV
PYDTDLEIPG FVDFIPEAWG HKCFYDGQLI GLLGQYKVQK EEEIVTGHIW SMPKYTSKKQ
GELKERLKHS YNALKKEFRK VFEETTNQEL ENLSEEEKNL LYEKKASAWY HVTYHPKWVK
KSLELLDPDE SSSHAVMLSF GWIAADYLAR IKIRSREVGS IDSAKPVDSL AKYLSQRL
//
