ID A0A087SAC1_AUXPR Unreviewed; 921 AA.
AC A0A087SAC1;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=4-alpha-glucanotransferase {ECO:0000256|ARBA:ARBA00012560};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE AltName: Full=Amylomaltase {ECO:0000256|ARBA:ARBA00031423};
DE AltName: Full=Disproportionating enzyme {ECO:0000256|ARBA:ARBA00031501};
GN ORFNames=F751_0471 {ECO:0000313|EMBL:KFM22675.1};
OS Auxenochlorella protothecoides (Green microalga) (Chlorella
OS protothecoides).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Auxenochlorella.
OX NCBI_TaxID=3075 {ECO:0000313|EMBL:KFM22675.1, ECO:0000313|Proteomes:UP000028924};
RN [1] {ECO:0000313|EMBL:KFM22675.1, ECO:0000313|Proteomes:UP000028924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0710 {ECO:0000313|EMBL:KFM22675.1,
RC ECO:0000313|Proteomes:UP000028924};
RX PubMed=25012212; DOI=10.1186/1471-2164-15-582;
RA Gao C., Wang Y., Shen Y., Yan D., He X., Dai J., Wu Q.;
RT "Oil accumulation mechanisms of the oleaginous microalga Chlorella
RT protothecoides revealed through its genome, transcriptomes, and
RT proteomes.";
RL BMC Genomics 15:582-582(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SIMILARITY: Belongs to the disproportionating enzyme family.
CC {ECO:0000256|ARBA:ARBA00005684}.
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DR EMBL; KL662079; KFM22675.1; -; Genomic_DNA.
DR RefSeq; XP_011395531.1; XM_011397229.1.
DR AlphaFoldDB; A0A087SAC1; -.
DR STRING; 3075.A0A087SAC1; -.
DR GeneID; 23611862; -.
DR KEGG; apro:F751_0471; -.
DR eggNOG; ENOG502QR3V; Eukaryota.
DR OrthoDB; 4592at2759; -.
DR Proteomes; UP000028924; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:2001070; F:starch binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR002044; CBM_fam20.
DR InterPro; IPR003385; Glyco_hydro_77.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR32518; -; 1.
DR PANTHER; PTHR32518:SF3; 4-ALPHA-GLUCANOTRANSFERASE; 1.
DR Pfam; PF00686; CBM_20; 1.
DR Pfam; PF02446; Glyco_hydro_77; 2.
DR SMART; SM01065; CBM_2; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 2.
DR PROSITE; PS51166; CBM20; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Reference proteome {ECO:0000313|Proteomes:UP000028924};
KW Transferase {ECO:0000313|EMBL:KFM22675.1}.
FT DOMAIN 113..227
FT /note="CBM20"
FT /evidence="ECO:0000259|PROSITE:PS51166"
SQ SEQUENCE 921 AA; 104658 MW; 5ABE95D0EC444DE0 CRC64;
MLGNLDVERG HKLECSHVGS TLVWQTSLRV AHPADLLYKY AVVDARLNVV KWESGSHTLH
LPADLVNDAI VTVTDVWEDD SHPAHLFARS AFRKAILGDH QTPRLAAEGP RPSICEGHAL
VKFQVRDWQL KEGQQVCVTG GAPGLGSWQL PQSLSLRLTA PSCWEVEVSI PHTSFPLTYK
YVIRGADGTL LLEAGENRMM PAPKEGPLPL LVVRNDSFFR HERRWRGAGV AVPVFSLRTE
ASVGCGDFAD LCPLLDFCRN TGLSVVQLLP VNDTSVKGDW RDSYPYSSLC VFALHPLYLC
LDRLPGAHAS AIQEEVAEAR RRLNLPDMDY EQTLAAKLAI ARKLFDAAFP QLLSDPAFLE
FCAHHEEWLK PYSMFCYFQS FFGTADHTQW GRYSAYDPRA TAKFWRPDSE SARDMWFCSY
LQYHLHCQLS AASAYARERH VVLKGDLPIG VDKCSVDTWL YPNLFHMGTG TGSPPDWFNQ
DGQNWGFPTY NWEEMAKDDY SWWRRRLAQM ARYFQACRRD LGGRKEECSL RVFRVDHIIG
FFRIWEIPGD CWSGMMGHFR PCIPIARAEL EAKGIWDFHR HVGHKEVELE EPYVTLALLT
AELGDVLAAE AASAFFEPSY GDRLRFREEY ASEVVLRTLT VPEGMSGLAV SAWPRIHETL
LLMRRNVLLL RDPEDPQKLY PRFDLTKTTS FAALDNVAWA EELAWQHDDY FYRRQEELWR
AQGHRTLPAL LSSSDMLMCG EDLGFTPACV RPIMEELGIL ALRIQRMPSS QEEFGDPADY
PYMCVASPSS HDTTTTRAWY EEDAPRRQRY YAQVLGGEGP APASCTPEIM RAIAQQHVDS
PAVLAIFPIQ DIIALETWDR VRPASEETIN APSNPEHYWR YRMHLTLREL DCPRLQATVQ
GLLLSSGRCL AGDLPRLMVE E
//
