ID A0A087SDN0_AUXPR Unreviewed; 275 AA.
AC A0A087SDN0;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=hydroxyethylthiazole kinase {ECO:0000256|ARBA:ARBA00012129};
DE EC=2.7.1.50 {ECO:0000256|ARBA:ARBA00012129};
GN ORFNames=APUTEX25_000334 {ECO:0000313|EMBL:RMZ54817.1}, F751_6802
GN {ECO:0000313|EMBL:KFM23834.1};
OS Auxenochlorella protothecoides (Green microalga) (Chlorella
OS protothecoides).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Auxenochlorella.
OX NCBI_TaxID=3075 {ECO:0000313|EMBL:KFM23834.1, ECO:0000313|Proteomes:UP000028924};
RN [1] {ECO:0000313|EMBL:KFM23834.1, ECO:0000313|Proteomes:UP000028924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0710 {ECO:0000313|EMBL:KFM23834.1,
RC ECO:0000313|Proteomes:UP000028924};
RX PubMed=25012212; DOI=10.1186/1471-2164-15-582;
RA Gao C., Wang Y., Shen Y., Yan D., He X., Dai J., Wu Q.;
RT "Oil accumulation mechanisms of the oleaginous microalga Chlorella
RT protothecoides revealed through its genome, transcriptomes, and
RT proteomes.";
RL BMC Genomics 15:582-582(2014).
RN [2] {ECO:0000313|Proteomes:UP000279271}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 25 {ECO:0000313|Proteomes:UP000279271};
RX DOI=10.1016/j.algal.2018.07.001;
RA Vogler B.W., Starkenburg S.R., Sudasinghe N., Schambach J.Y., Rollin J.A.,
RA Pattathil S., Barry A.N.;
RT "Characterization of plant carbon substrate utilization by Auxenochlorella
RT protothecoides.";
RL Algal Res. 34:37-48(2018).
RN [3] {ECO:0000313|EMBL:RMZ54817.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 25 {ECO:0000313|EMBL:RMZ54817.1};
RA Hovde B., Zhang X.;
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:RMZ54817.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 25 {ECO:0000313|EMBL:RMZ54817.1};
RA Vogler B.W., Starkenburg S.R., Sudasinghe N., Schambach J.Y., Rollin J.A.,
RA Pattathil S., Barry A.N.;
RT "Characterization of plant carbon substrate utilization by Auxenochlorella
RT protothecoides.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-
CC phosphooxyethyl)-thiazole + ADP + H(+); Xref=Rhea:RHEA:24212,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17957, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:58296, ChEBI:CHEBI:456216; EC=2.7.1.50;
CC Evidence={ECO:0000256|ARBA:ARBA00001771};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-
CC methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-
CC methylthiazole: step 1/1. {ECO:0000256|ARBA:ARBA00004868}.
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DR EMBL; KL662101; KFM23834.1; -; Genomic_DNA.
DR EMBL; QOKY01000172; RMZ54817.1; -; Genomic_DNA.
DR RefSeq; XP_011396712.1; XM_011398410.1.
DR STRING; 3075.A0A087SDN0; -.
DR GeneID; 23618193; -.
DR KEGG; apro:F751_6802; -.
DR eggNOG; ENOG502QS2M; Eukaryota.
DR OrthoDB; 2784451at2759; -.
DR UniPathway; UPA00060; UER00139.
DR Proteomes; UP000028924; Unassembled WGS sequence.
DR Proteomes; UP000279271; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004417; F:hydroxyethylthiazole kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01170; THZ_kinase; 1.
DR Gene3D; 3.40.1190.20; -; 1.
DR HAMAP; MF_00228; Thz_kinase; 1.
DR InterPro; IPR000417; Hyethyz_kinase.
DR InterPro; IPR029056; Ribokinase-like.
DR NCBIfam; TIGR00694; thiM; 1.
DR Pfam; PF02110; HK; 1.
DR PIRSF; PIRSF000513; Thz_kinase; 1.
DR PRINTS; PR01099; HYETHTZKNASE.
DR SUPFAM; SSF53613; Ribokinase-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Kinase {ECO:0000313|EMBL:KFM23834.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000028924};
KW Thiamine biosynthesis {ECO:0000256|ARBA:ARBA00022977};
KW Transferase {ECO:0000313|EMBL:KFM23834.1}.
SQ SEQUENCE 275 AA; 28152 MW; D16F09B7F18CE7B0 CRC64;
MGLTGRATAL LLEKCREENP LVQCITNFVS MDLMANTLLA AGASPAMAHS ISEVESFVEL
ASALLVNVGT LSEDWAGGMK LAALTAGRLR KPWVLDPVAC GATPARTSLC KDLMALGPTV
IRGNASEIMA LASLGGERTR GVDSSHPSEA AAPMARALAA RQGCIVAVSG AVDVVTDGTR
TLLVGNGHPL LQKVTATGCS VTALIAAFVA VAGPEQALEA TAHALAYFGV AAERAAVDEA
GQVRGPGSFR VKLLDELDLL CAAQLVHASR IGRST
//
