ID A0A087SFD9_AUXPR Unreviewed; 501 AA.
AC A0A087SFD9;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE SubName: Full=Cytosolic purine 5'-nucleotidase {ECO:0000313|EMBL:KFM24443.1};
GN ORFNames=F751_3161 {ECO:0000313|EMBL:KFM24443.1};
OS Auxenochlorella protothecoides (Green microalga) (Chlorella
OS protothecoides).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Auxenochlorella.
OX NCBI_TaxID=3075 {ECO:0000313|EMBL:KFM24443.1, ECO:0000313|Proteomes:UP000028924};
RN [1] {ECO:0000313|EMBL:KFM24443.1, ECO:0000313|Proteomes:UP000028924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0710 {ECO:0000313|EMBL:KFM24443.1,
RC ECO:0000313|Proteomes:UP000028924};
RX PubMed=25012212; DOI=10.1186/1471-2164-15-582;
RA Gao C., Wang Y., Shen Y., Yan D., He X., Dai J., Wu Q.;
RT "Oil accumulation mechanisms of the oleaginous microalga Chlorella
RT protothecoides revealed through its genome, transcriptomes, and
RT proteomes.";
RL BMC Genomics 15:582-582(2014).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
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DR EMBL; KL662107; KFM24443.1; -; Genomic_DNA.
DR RefSeq; XP_011397331.1; XM_011399029.1.
DR AlphaFoldDB; A0A087SFD9; -.
DR GeneID; 23614552; -.
DR KEGG; apro:F751_3161; -.
DR eggNOG; KOG2469; Eukaryota.
DR OrthoDB; 3626840at2759; -.
DR Proteomes; UP000028924; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07522; HAD_cN-II; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 2.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF15; CYTOSOLIC PURINE 5'-NUCLEOTIDASE; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000028924}.
FT REGION 324..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 52
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 54
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 52
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 54
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 372
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 501 AA; 55937 MW; A80F72AFCAB9272F CRC64;
MSAVFPPPPP LASEANPLWT DDAAEEPCMP PSRRIFCNRS LNMAAVRAVG FDMDYTLAQY
KPETFEVLAH DETVKKLVSA FGYPKSLLDL EFDWRYMSRG LVIDKQRGNM LKVDRHKYVK
LAYHGFSELP RAARLATYND APQRHGYDEP AYALIDTLFS LAEAHLFMQL VELLDAGGSG
LPLGKQYADL YRDVRGAVDL CHRDGSIKRE VAADPARYIH ADPSLGPVLG ALRASGKRVF
LATNSLWDYT HVVMNYLVEG RVGAARSTSW LSRFDVVVTG CGKPRFFAER KDLFEVHPPS
GMLWNTEGGS PMVPIGEDDL PSAALGSTAP SARDVAPPRP GGEARVFQGG SFHDLHKMLG
VAAGSEVLYV GDHIYGDVLR SKKALGWRTM LVIPELESEL QGLAERGGDM AELRRLRRMR
DAVDDRLQRD RLRGLHQSLL QRHHEHFHPV WGQIERFACL YTSHVANLMF YSPDKSYKAR
PDTMCHEDEL APASELPPPC P
//
