ID A0A087SN26_AUXPR Unreviewed; 1048 AA.
AC A0A087SN26;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=isochorismate synthase {ECO:0000256|ARBA:ARBA00012824};
DE EC=5.4.4.2 {ECO:0000256|ARBA:ARBA00012824};
GN ORFNames=F751_1303 {ECO:0000313|EMBL:KFM27130.1};
OS Auxenochlorella protothecoides (Green microalga) (Chlorella
OS protothecoides).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Auxenochlorella.
OX NCBI_TaxID=3075 {ECO:0000313|EMBL:KFM27130.1, ECO:0000313|Proteomes:UP000028924};
RN [1] {ECO:0000313|EMBL:KFM27130.1, ECO:0000313|Proteomes:UP000028924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0710 {ECO:0000313|EMBL:KFM27130.1,
RC ECO:0000313|Proteomes:UP000028924};
RX PubMed=25012212; DOI=10.1186/1471-2164-15-582;
RA Gao C., Wang Y., Shen Y., Yan D., He X., Dai J., Wu Q.;
RT "Oil accumulation mechanisms of the oleaginous microalga Chlorella
RT protothecoides revealed through its genome, transcriptomes, and
RT proteomes.";
RL BMC Genomics 15:582-582(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chorismate = isochorismate; Xref=Rhea:RHEA:18985,
CC ChEBI:CHEBI:29748, ChEBI:CHEBI:29780; EC=5.4.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000799};
CC -!- SIMILARITY: Belongs to the isochorismate synthase family.
CC {ECO:0000256|ARBA:ARBA00005297}.
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DR EMBL; KL662143; KFM27130.1; -; Genomic_DNA.
DR RefSeq; XP_011400097.1; XM_011401795.1.
DR AlphaFoldDB; A0A087SN26; -.
DR STRING; 3075.A0A087SN26; -.
DR GeneID; 23612694; -.
DR KEGG; apro:F751_1303; -.
DR eggNOG; KOG1223; Eukaryota.
DR OrthoDB; 298598at2759; -.
DR Proteomes; UP000028924; Unassembled WGS sequence.
DR GO; GO:0070204; F:2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity; IEA:InterPro.
DR GO; GO:0008909; F:isochorismate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR GO; GO:0009234; P:menaquinone biosynthetic process; IEA:InterPro.
DR CDD; cd07037; TPP_PYR_MenD; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR Gene3D; 3.60.120.10; Anthranilate synthase; 1.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR HAMAP; MF_01659; MenD; 1.
DR InterPro; IPR005801; ADC_synthase.
DR InterPro; IPR015890; Chorismate_C.
DR InterPro; IPR004561; IsoChor_synthase.
DR InterPro; IPR004433; MenaQ_synth_MenD.
DR InterPro; IPR032264; MenD_middle.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR NCBIfam; TIGR00543; isochor_syn; 1.
DR NCBIfam; TIGR00173; menD; 1.
DR PANTHER; PTHR42916; 2-SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3-CYCLOHEXENE-1-CARBOXYLATE SYNTHASE; 1.
DR PANTHER; PTHR42916:SF1; PROTEIN PHYLLO, CHLOROPLASTIC; 1.
DR Pfam; PF00425; Chorismate_bind; 1.
DR Pfam; PF16582; TPP_enzyme_M_2; 1.
DR Pfam; PF02776; TPP_enzyme_N; 1.
DR SUPFAM; SSF56322; ADC synthase; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Reference proteome {ECO:0000313|Proteomes:UP000028924}.
FT DOMAIN 258..511
FT /note="Chorismate-utilising enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00425"
FT DOMAIN 545..656
FT /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF02776"
FT DOMAIN 719..959
FT /note="Menaquinone biosynthesis protein MenD middle"
FT /evidence="ECO:0000259|Pfam:PF16582"
SQ SEQUENCE 1048 AA; 108722 MW; A773FAE41C791421 CRC64;
MPAPSPPPPP VHVHCQTLPP CKDLAAGKEQ VEKALDAAVA ARAGLPAALI RCEAQVPRTH
SALAWLQAQR AGTSGAAAPS RPAPTLYFSP RLSPGPDSAG AAAAACEEGA GAVAGVGAAA
LWRGAPGEAL DAARLHGMQR FTSAAAPRVR ALGGSRFDAQ RGLSPEWAAF GTFTFLIPSV
EAAVTLAQEL LDEESAEGEA APCTPSLLDP GTALENYIAG GQQGLDDLLE ALEGTPALGA
PATGGDCGTR DPDCGAAALS KVVLARRSEL RLSGGADPLA LLASLQARNP RAYALFLELE
GGEAFLSCTP ERLYSRSGRS VASEAVAGTR ARGGGSVEND FWLALDLLQS KKEHAEFTIV
RDWVYEALRG ICATVRVDVR KTVLKQESLQ HLYGQLSGTL LPAATDADLL AALHPTPAVC
GRPREASRDM LSSLEPFDRG FYSGPFGWLG GQGAEFAVAI RSALFVPEVQ APEFPGVQAL
GPTAMRVALY AGVGIVPGSK ASLEWAELDL KVAALDRLLT PVPALQQARK RGSQWGAPNL
NAAYARILVE ELCRLGCSTF CIAPGSRSSP LTLAAAQHPR VQLVSGMDER SLAFWALGHG
RSGAAPAVIL TTSGTAVANL LPAAVEGWQS GTPLIILTAD RPAEVRGSGA NQTIHQPGIF
SSYVCWQADL APPSSAMPPQ HLLGSLDRAV AAAGAGPVHL NVQLREPLGP VPEPWESTAW
LEGLGDWATS SRPWSVQLEA AACASRLPHS LLAALSTATR GLLVAGELRA PEDRAAALAV
SRALRWPLLA DVLSGLRVGA TGVPEGLIAC GDNILAAQDP GMCTALRPDV VLQLGGRLTS
KRISAFLAAS SARGSSAGND SAAWWVVDAS PHSFDEHGCL AGRVRLPLGA LERLLREAGV
LVEGAAAAPF TSPYLELWRT MDREVGFLVR QRLNSAPYGE AHIAACLASS LPRGHALYIG
NSMPIRDMDM FADGLALSGC TQGQVPSLGC PVGANRGASG IDGVLSSAAG FASGLGMPTT
LVVGDLSFLH DTNGLMLLRS GKETWDRA
//
