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Database: UniProt
Entry: A0A087STN1_AUXPR
LinkDB: A0A087STN1_AUXPR
Original site: A0A087STN1_AUXPR 
ID   A0A087STN1_AUXPR        Unreviewed;      1099 AA.
AC   A0A087STN1;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   05-JUN-2019, entry version 24.
DE   RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE            EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN   ORFNames=APUTEX25_000666 {ECO:0000313|EMBL:RMZ52391.1}, F751_3702
GN   {ECO:0000313|EMBL:KFM29085.1};
OS   Auxenochlorella protothecoides (Green microalga) (Chlorella
OS   protothecoides).
OC   Eukaryota; Viridiplantae; Chlorophyta; Trebouxiophyceae; Chlorellales;
OC   Chlorellaceae; Auxenochlorella.
OX   NCBI_TaxID=3075 {ECO:0000313|EMBL:KFM29085.1, ECO:0000313|Proteomes:UP000028924};
RN   [1] {ECO:0000313|EMBL:KFM29085.1, ECO:0000313|Proteomes:UP000028924}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=0710 {ECO:0000313|EMBL:KFM29085.1,
RC   ECO:0000313|Proteomes:UP000028924};
RX   PubMed=25012212; DOI=10.1186/1471-2164-15-582;
RA   Gao C., Wang Y., Shen Y., Yan D., He X., Dai J., Wu Q.;
RT   "Oil accumulation mechanisms of the oleaginous microalga Chlorella
RT   protothecoides revealed through its genome, transcriptomes, and
RT   proteomes.";
RL   BMC Genomics 15:582-582(2014).
RN   [2] {ECO:0000313|Proteomes:UP000279271}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 25 {ECO:0000313|Proteomes:UP000279271};
RX   DOI=10.1016/j.algal.2018.07.001;
RA   Vogler B.W., Starkenburg S.R., Sudasinghe N., Schambach J.Y.,
RA   Rollin J.A., Pattathil S., Barry A.N.;
RT   "Characterization of plant carbon substrate utilization by
RT   Auxenochlorella protothecoides.";
RL   Algal Res 34:37-48(2018).
RN   [3] {ECO:0000313|EMBL:RMZ52391.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 25 {ECO:0000313|EMBL:RMZ52391.1};
RA   Hovde B., Zhang X.;
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:RMZ52391.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 25 {ECO:0000313|EMBL:RMZ52391.1};
RA   Vogler B.W., Starkenburg S.R., Sudasinghe N., Schambach J.Y.,
RA   Rollin J.A., Pattathil S., Barry A.N.;
RT   "Characterization of plant carbon substrate utilization by
RT   Auxenochlorella protothecoides.";
RL   Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|RuleBase:RU000442};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|RuleBase:RU000442};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU000442}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC       {ECO:0000256|RuleBase:RU000442}.
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DR   EMBL; KL662186; KFM29085.1; -; Genomic_DNA.
DR   EMBL; QOKY01000206; RMZ52391.1; -; Genomic_DNA.
DR   RefSeq; XP_011402134.1; XM_011403832.1.
DR   STRING; 3075.A0A087STN1; -.
DR   GeneID; 23615093; -.
DR   KEGG; apro:F751_3702; -.
DR   KO; K02327; -.
DR   OrthoDB; 20210at2759; -.
DR   Proteomes; UP000028924; Unassembled WGS sequence.
DR   Proteomes; UP000279271; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR042087; DNA_pol_B_C.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR025687; Znf-C4pol.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   Pfam; PF14260; zf-C4pol; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|RuleBase:RU000442};
KW   Complete proteome {ECO:0000313|Proteomes:UP000028924,
KW   ECO:0000313|Proteomes:UP000279271};
KW   DNA replication {ECO:0000256|RuleBase:RU000442};
KW   DNA-binding {ECO:0000256|RuleBase:RU000442};
KW   DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU000442};
KW   Iron {ECO:0000256|RuleBase:RU000442};
KW   Iron-sulfur {ECO:0000256|RuleBase:RU000442};
KW   Metal-binding {ECO:0000256|RuleBase:RU000442};
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU000442};
KW   Nucleus {ECO:0000256|RuleBase:RU000442};
KW   Reference proteome {ECO:0000313|Proteomes:UP000028924};
KW   Transferase {ECO:0000256|RuleBase:RU000442};
KW   Zinc {ECO:0000256|RuleBase:RU000442};
KW   Zinc-finger {ECO:0000256|RuleBase:RU000442}.
FT   DOMAIN      115    450       DNA_pol_B_exo1. {ECO:0000259|Pfam:
FT                                PF03104}.
FT   DOMAIN      538    970       DNA_pol_B. {ECO:0000259|Pfam:PF00136}.
FT   DOMAIN     1008   1079       zf-C4pol. {ECO:0000259|Pfam:PF14260}.
FT   REGION        1     41       Disordered. {ECO:0000256|MobiDB-lite:
FT                                A0A087STN1}.
SQ   SEQUENCE   1099 AA;  121501 MW;  E29B2B9B0BEC360C CRC64;
     MSAAHVKRPA TVMEGKENLP SKRPAPEVGP EVPAPVPDFF DVTDDGPEVV EEIEEGALGE
     AGRNWPRPPA APLDASRDKL VFQQLELDYT SGPPNKRFYN AQLREVPIIR MFGVTELGNS
     VCLFVHGFEP YFYIESPRPT FSPDDCRALV EMFNNLLATK DRSGNAQCCL RIEVVQKQSM
     MYYQPAKSRA FLKVVMALPG LVAPARGLVE GGLWLEWLAC RLPQTTYESN VLYALRFMVD
     TGIVGGNWVE LPAGKYVLTP GPSQLSHCQL EAHIAAADLV SHAPEGAWAK LAPFRILSVD
     IECQGRKGHF PEAQHDPVIQ IASLVTEMGK ATPAVRNVMT LRDCSPIAGA EVMSFEDEKD
     LLMRWRDLII ETDPDVIIGY NIIGFDLPYL LDRATALGVP QFWAWGKLRN RSKAYGTHEY
     KEVGIEGRVQ FDLLQAIRRD HNLSSYSLNS GECMTGLDCL WMSAHFLGEQ KEDVHHSDIS
     KLQDGNSETR RRLAIYCLKV PREGVVVLGE GDAYLPQRLL DKLMCMYNYI EMARVTGVPM
     SFLLARGQSI KVFSQILRKT RARGLLRGGQ GEDGVAYEGA TVLEPKAGYY ATPVATLDFA
     SLYPSIMMAH NLCYTTLLPR GAHASFPATG EGARAVESPS GERFVKPELQ AGVLPEILRE
     LLSARKRARA DMASATDPFL KAVFNGRQLA LKISANSVYG FTGATVGALP CLEISSSVTS
     FGRAMIEATK QKVMSTFNKA SGYPADADVI YGDTDSVMVN FGVPDTATAM ALGQEAADLV
     SKTFVQPIKL EFEKVYNPYL LISKKRYAGL LWTRAEAWDK IDTKGIETVR RDNCLLVRNV
     ITTCLDMILV QRSPERAAEY VKGVIADLLM NKLDLSLLVV SKGLTQDAAD YETKAAHVEL
     AKKMKKRDPA TAPAMGDRVP YVIIKAARGA KAYEKAEDPL YVLRNNLPID SQHYLDHYLA
     QPLMRLFEPI LRNPRDLLCG AHTRAILVST PSSGSGGIMR FAQKKVTCLG CKAALGPGEK
     TVCKHCQPKE GEVYCKTVAA VNDLETQFAA LWTQCQRCQG SLHQDVLCRS RDCPIFYRRM
     KVQKEVGEAA ASLARFDEW
//
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