ID A0A087VT68_9BIFI Unreviewed; 282 AA.
AC A0A087VT68;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=2,5-diketo-D-gluconate reductase {ECO:0000313|EMBL:AIC91533.1};
DE EC=1.1.1.218 {ECO:0000313|EMBL:AIC91533.1};
GN ORFNames=BINDI_0248 {ECO:0000313|EMBL:AIC91533.1};
OS Bifidobacterium indicum LMG 11587 = DSM 20214.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=1341694 {ECO:0000313|EMBL:AIC91533.1, ECO:0000313|Proteomes:UP000028569};
RN [1] {ECO:0000313|EMBL:AIC91533.1, ECO:0000313|Proteomes:UP000028569}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 11587 {ECO:0000313|EMBL:AIC91533.1,
RC ECO:0000313|Proteomes:UP000028569};
RX PubMed=25085493; DOI=10.1128/AEM.02308-14;
RA Milani C., Lugli G.A., Duranti S., Turroni F., Bottacini F., Mangifesta M.,
RA Sanchez B., Viappiani A., Mancabelli L., Taminiau B., Delcenserie V.,
RA Barrangou R., Margolles A., van Sinderen D., Ventura M.;
RT "Genomic encyclopedia of type strains of the genus Bifidobacterium.";
RL Appl. Environ. Microbiol. 80:6290-6302(2014).
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DR EMBL; CP006018; AIC91533.1; -; Genomic_DNA.
DR RefSeq; WP_033491675.1; NZ_JDUE01000011.1.
DR AlphaFoldDB; A0A087VT68; -.
DR KEGG; bii:BINDI_0248; -.
DR HOGENOM; CLU_023205_0_1_11; -.
DR OrthoDB; 9804790at2; -.
DR Proteomes; UP000028569; Chromosome.
DR GO; GO:0050109; F:morphine 6-dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19071; AKR_AKR1-5-like; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:AIC91533.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000028569}.
FT DOMAIN 23..266
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 51
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 113
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 80
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 282 AA; 31636 MW; 454362BE733CFD8A CRC64;
MAAVPDIELA DGRRIPQIGL GVLRIADDQT GQVVESALSL GYRHIDTAAG YQNEAGVGRG
LKAAGYDTGS RRESIWLTTK LRDSEQGYDS ALRAFDRQLG LLGTDYVDMY MIHWPTPFDW
RAAETWKAFR ELRREKRVRT LGVCNFLEED LERLHRETGE YPAVNQIELH PTWQQRDLVA
FCRSRKIAVE AYSPLARGAD LSAGQGVIDR IAEAKGCSPA QVVLRWHIEN GTIIIPKSVH
AERQKENLDL FGFSLTEQER AAIDALDGPV RAGHDPRTYS YA
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