ID A0A087YHH3_POEFO Unreviewed; 859 AA.
AC A0A087YHH3;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 2.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=Mitogen-activated protein kinase kinase kinase kinase 1 {ECO:0000256|PIRNR:PIRNR038172};
DE EC=2.7.11.1 {ECO:0000256|PIRNR:PIRNR038172};
OS Poecilia formosa (Amazon molly) (Limia formosa).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Poeciliidae; Poeciliinae;
OC Poecilia.
OX NCBI_TaxID=48698 {ECO:0000313|Ensembl:ENSPFOP00000017476.2, ECO:0000313|Proteomes:UP000028760};
RN [1] {ECO:0000313|Proteomes:UP000028760}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000028760};
RA Schartl M., Warren W.;
RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSPFOP00000017476.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: May play a role in the response to environmental stress.
CC Appears to act upstream of the JUN N-terminal pathway.
CC {ECO:0000256|PIRNR:PIRNR038172}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433,
CC ECO:0000256|PIRNR:PIRNR038172};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775,
CC ECO:0000256|PIRNR:PIRNR038172};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|PIRNR:PIRNR038172};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC protein kinase family. STE20 subfamily. {ECO:0000256|ARBA:ARBA00008874,
CC ECO:0000256|PIRNR:PIRNR038172}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AYCK01004936; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AYCK01004937; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_007552530.1; XM_007552468.2.
DR AlphaFoldDB; A0A087YHH3; -.
DR STRING; 48698.ENSPFOP00000017476; -.
DR Ensembl; ENSPFOT00000017498.2; ENSPFOP00000017476.2; ENSPFOG00000017317.2.
DR GeneID; 103138324; -.
DR KEGG; pfor:103138324; -.
DR CTD; 11184; -.
DR eggNOG; KOG0576; Eukaryota.
DR GeneTree; ENSGT00940000160308; -.
DR OMA; IQVFWKH; -.
DR OrthoDB; 152877at2759; -.
DR Proteomes; UP000028760; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008349; F:MAP kinase kinase kinase kinase activity; IEA:InterPro.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd06613; STKc_MAP4K3_like; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR001180; CNH_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR021160; MAPKKKK.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR PANTHER; PTHR48012:SF15; MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE 1; 1.
DR PANTHER; PTHR48012; STERILE20-LIKE KINASE, ISOFORM B-RELATED; 1.
DR Pfam; PF00780; CNH; 1.
DR Pfam; PF00069; Pkinase; 1.
DR PIRSF; PIRSF038172; MAPKKKK; 1.
DR SMART; SM00036; CNH; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS50219; CNH; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR038172};
KW Kinase {ECO:0000256|PIRNR:PIRNR038172};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR038172};
KW Reference proteome {ECO:0000313|Proteomes:UP000028760};
KW Serine/threonine-protein kinase {ECO:0000256|PIRNR:PIRNR038172};
KW Transferase {ECO:0000256|PIRNR:PIRNR038172}.
FT DOMAIN 26..283
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT DOMAIN 527..829
FT /note="CNH"
FT /evidence="ECO:0000259|PROSITE:PS50219"
FT REGION 331..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..379
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..437
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 489..503
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 146
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR038172-1"
FT BINDING 32..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR038172-2"
FT BINDING 55
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR038172-2,
FT ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 859 AA; 96944 MW; B96EA5D1D3D0037A CRC64;
MSQHIRMDFH QRAALDISTK NPQDDFEILL RVGGGTYGEV YKARNKQNGE LAAIKVIKME
PEDDFSIIQQ EIVIVKSCKH PNIVAYYGSY IRANKLWICM EFCGGGSLQD IYHVTGSLSE
PQIAYVCREM LQGLDYLHAQ KKIHRDIKGA NILLNDQGEV KLADFGISAQ ITATLARRMS
FIGTPYWMAP EVAAVEIKGG YNELCDVWSV GITAIELAEL QPPLFDVHPL RVLFLMSKSG
YQPPKLKDKS KWSSNFYNFV KAMLVRNPKK RPSAAKMLSH PFLTQQCLNQ ELTLDLLDKF
KNPEKHKSCV STEDDDMEVA PPASLRRIQS INKHNRAERT NSDISFEQIY TQRPMKTGSP
NVTLTPSSGS TGSINSPSRD QDSDSDDYDD VDIPTMQPSG LIMPEDETPP PLPPKPKART
SSEESMASED DRSRKHGSLC PPAPAPLIRT SSGTHVRPTP HPRASRHSDP PSLPTQIQNI
PADTVPPELP PKDFRRRKPI SKDLPECPSP VLKKPPVYFK KIFHGCPLKI NCSTTWENPT
SKEQHLIMGA EEGIYTLNLN GSEATMELLY PGKCSWVYTI NNVLMSVSGK SSQLHSHSLK
ELYEQARKDQ RMVPLPTHRL LSRKCPVTSK IPDTKGCKTC AVGGNLFLCC ALDSSVVLLQ
WYEPMHKFML IKHFDFPLPS PLRVFEMVVP PLQEYPMVCI GVSRSCSPST PVNVEYINLN
SNTSWFFNSG MEKPCPDVVQ VNQLDGNSLL VLMERSVQVV NLEGELKTNR HPPQETIFSH
DVESIVYFED TLLAVWRHGW QRRTKSFTEV LDEMTDHRKI YRMVKSDRMV VLETRQVEDQ
LGLSNLYLLE VAENYVLLP
//
