ID A0A088T1J5_9BACT Unreviewed; 216 AA.
AC A0A088T1J5;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 13-SEP-2023, entry version 41.
DE RecName: Full=Uracil-DNA glycosylase {ECO:0000256|ARBA:ARBA00012030, ECO:0000256|HAMAP-Rule:MF_00148};
DE Short=UDG {ECO:0000256|HAMAP-Rule:MF_00148};
DE EC=3.2.2.27 {ECO:0000256|ARBA:ARBA00012030, ECO:0000256|HAMAP-Rule:MF_00148};
GN Name=ung {ECO:0000256|HAMAP-Rule:MF_00148,
GN ECO:0000313|EMBL:AIO18813.1};
GN ORFNames=KQ51_00933 {ECO:0000313|EMBL:AIO18813.1};
OS Candidatus Izimaplasma bacterium HR1.
OC Bacteria; Mycoplasmatota; Candidatus Izimaplasma.
OX NCBI_TaxID=1541959 {ECO:0000313|EMBL:AIO18813.1, ECO:0000313|Proteomes:UP000029408};
RN [1] {ECO:0000313|Proteomes:UP000029408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HR1 {ECO:0000313|Proteomes:UP000029408};
RA Skennerton C.T., Haroon M.F., Tyson G.W., Orphan V.J.;
RT "Phylogenomic Analysis of Candidatus Izimaplasma species: Representatives
RT from a Novel Mollicute Clade found in Ocean Sediments.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AIO18813.1, ECO:0000313|Proteomes:UP000029408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HR1 {ECO:0000313|EMBL:AIO18813.1,
RC ECO:0000313|Proteomes:UP000029408};
RX PubMed=27058507; DOI=10.1038/ismej.2016.55;
RA Skennerton C.T., Haroon M.F., Briegel A., Shi J., Jensen G.J., Tyson G.W.,
RA Orphan V.J.;
RT "Phylogenomic analysis of Candidatus 'Izimaplasma' species: free-living
RT representatives from a Tenericutes clade found in methane seeps.";
RL ISME J. 10:2679-2692(2016).
CC -!- FUNCTION: Excises uracil residues from the DNA which can arise as a
CC result of misincorporation of dUMP residues by DNA polymerase or due to
CC deamination of cytosine. {ECO:0000256|ARBA:ARBA00002631,
CC ECO:0000256|HAMAP-Rule:MF_00148, ECO:0000256|RuleBase:RU003780}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes single-stranded DNA or mismatched double-stranded
CC DNA and polynucleotides, releasing free uracil.; EC=3.2.2.27;
CC Evidence={ECO:0000256|ARBA:ARBA00001400, ECO:0000256|HAMAP-
CC Rule:MF_00148, ECO:0000256|RuleBase:RU003780};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00148}.
CC -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC UNG family. {ECO:0000256|ARBA:ARBA00008184, ECO:0000256|HAMAP-
CC Rule:MF_00148, ECO:0000256|RuleBase:RU003780}.
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DR EMBL; CP009415; AIO18813.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A088T1J5; -.
DR STRING; 1541959.KQ51_00933; -.
DR KEGG; mbj:KQ51_00933; -.
DR PATRIC; fig|1541959.3.peg.891; -.
DR eggNOG; COG0692; Bacteria.
DR HOGENOM; CLU_032162_3_0_14; -.
DR OrthoDB; 9804372at2; -.
DR Proteomes; UP000029408; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004844; F:uracil DNA N-glycosylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006284; P:base-excision repair; IEA:UniProtKB-UniRule.
DR CDD; cd10027; UDG-F1-like; 1.
DR Gene3D; 3.40.470.10; Uracil-DNA glycosylase-like domain; 1.
DR HAMAP; MF_00148; UDG; 1.
DR InterPro; IPR002043; UDG_fam1.
DR InterPro; IPR018085; Ura-DNA_Glyclase_AS.
DR InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR NCBIfam; TIGR00628; ung; 1.
DR PANTHER; PTHR11264; URACIL-DNA GLYCOSYLASE; 1.
DR PANTHER; PTHR11264:SF0; URACIL-DNA GLYCOSYLASE; 1.
DR Pfam; PF03167; UDG; 1.
DR SMART; SM00986; UDG; 1.
DR SMART; SM00987; UreE_C; 1.
DR SUPFAM; SSF52141; Uracil-DNA glycosylase-like; 1.
DR PROSITE; PS00130; U_DNA_GLYCOSYLASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00148};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00148};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00148}; Glycosidase {ECO:0000313|EMBL:AIO18813.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00148};
KW Reference proteome {ECO:0000313|Proteomes:UP000029408}.
FT DOMAIN 43..203
FT /note="Uracil-DNA glycosylase-like"
FT /evidence="ECO:0000259|SMART:SM00986"
FT ACT_SITE 58
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00148,
FT ECO:0000256|PROSITE-ProRule:PRU10072"
SQ SEQUENCE 216 AA; 25308 MW; 0EE56345E86D2420 CRC64;
MWHDYIEKEK QKDYFKKLED FTEKEYETKE IFPPYKDIFN AFNYCPFVDT KVIILGQDPY
HDFNQAHGLA FSVKKGNKIP PSLRNIYKEL NSDLGLSVPE HGELTNWAKQ GILLLNTILT
VEAHKPMSHS KKGWETFTDN VIRELNKDSK PKVFVLWGNN ARKKNILIDN PKHLIIETSH
PSPLSARHSF FGSKVFTKIN DFLFNNYRSI IDFELE
//
