ID A0A089IHU3_9BACL Unreviewed; 610 AA.
AC A0A089IHU3;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=H70737_07595 {ECO:0000313|EMBL:AIQ22730.1};
OS Paenibacillus sp. FSL H7-0737.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1536775 {ECO:0000313|EMBL:AIQ22730.1, ECO:0000313|Proteomes:UP000029519};
RN [1] {ECO:0000313|EMBL:AIQ22730.1, ECO:0000313|Proteomes:UP000029519}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FSL H7-0737 {ECO:0000313|EMBL:AIQ22730.1,
RC ECO:0000313|Proteomes:UP000029519};
RA den Bakker H.C., Tsai Y.-C., Martin N., Korlach J., Wiedmann M.;
RT "Comparative genomics of the Paenibacillus odorifer group.";
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; CP009279; AIQ22730.1; -; Genomic_DNA.
DR RefSeq; WP_042186018.1; NZ_CP009279.1.
DR AlphaFoldDB; A0A089IHU3; -.
DR KEGG; paej:H70737_07595; -.
DR eggNOG; COG1874; Bacteria.
DR HOGENOM; CLU_007853_7_2_9; -.
DR Proteomes; UP000029519; Chromosome.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF199; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000029519}.
FT DOMAIN 12..323
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 374..480
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 509..567
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 158
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 234
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 610 AA; 68864 MW; FE8BCCEF4B3B323D CRC64;
MSLLTWNDGQ YFLDGQPYRI VSGAIHYFRV VPEYWKDRLL KLKACGFNTV ETYVAWNMHE
PKEGEFVFDG MADVVSFIEL AGELGLHVIV RPSPFICAEW EFGGLPAWLL AYSELQLRCC
DPLYLSKVDR YYDELIPRLV PLLSTHGGPI LAVQVENEYG SYGNDSSYLR YMRNGLLSRG
VDVLLFTSDG PTDEMLLGGT LEDVHATVNF GSCAEESFRK YREYRSEEPL MVMEYWNGWF
DHWMEDHHVR DAEDVAGVLD DILTHGSSLN MYMFHGGTNF GFYNGANHIV AYEPTTTSYD
YDAPLTEWGD TTTKFEAVRS VLGKHGFAPT CPLPEPIPKI SYGKVALMER AELFTASNLE
KLSEPVNSVC VQPMEKLGQS YGFILYSTYV QGPRTRQKLY IEGVRDRAQV FLDGQVLGVI
ERWNPQPLEL SIPAGGAQLD ILVENMGRIN YGPLIRDPKG ILDGVRIDNQ LQYNWTIRTL
PLEPGTLASL EYKEQTVEKQ EAAAPSGLPG FYRGCFEAEE IGDTFLRFDG WNKGVAWING
FNLGRYWNAG PQRALYIPGP LLRKGRNKLV LLELHGCAET CEIELVNCPD LGRTSAVDDA
VLNFVQEDNL
//
