ID A0A089KUU9_9BACL Unreviewed; 886 AA.
AC A0A089KUU9;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=R70331_03880 {ECO:0000313|EMBL:AIQ50748.1};
OS Paenibacillus sp. FSL R7-0331.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1536773 {ECO:0000313|EMBL:AIQ50748.1, ECO:0000313|Proteomes:UP000029487};
RN [1] {ECO:0000313|EMBL:AIQ50748.1, ECO:0000313|Proteomes:UP000029487}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FSL R7-0331 {ECO:0000313|EMBL:AIQ50748.1,
RC ECO:0000313|Proteomes:UP000029487};
RA den Bakker H.C., Tsai Y.-C., Martin N., Korlach J., Wiedmann M.;
RT "Comparative genomics of the Paenibacillus odorifer group.";
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
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DR EMBL; CP009284; AIQ50748.1; -; Genomic_DNA.
DR RefSeq; WP_042172966.1; NZ_CP009284.1.
DR AlphaFoldDB; A0A089KUU9; -.
DR STRING; 1536773.R70331_03880; -.
DR KEGG; paee:R70331_03880; -.
DR eggNOG; COG3250; Bacteria.
DR HOGENOM; CLU_005015_3_2_9; -.
DR Proteomes; UP000029487; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 3.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 3.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000029487};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 62..127
FT /note="Glycosyl hydrolases family 2 sugar binding"
FT /evidence="ECO:0000259|Pfam:PF02837"
FT DOMAIN 199..301
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 670..759
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 763..822
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 886 AA; 99128 MW; DEDF7291DE7E5E88 CRC64;
MTHKQLVLDN WEFRASGDES WLPAAVPGTV HTDLLRSGLI DQPFYGTNEH RLQWIDKKDW
EYRTTLQLDE GWESLAVTEL NFAGLDTYAD VYVNNVHALS ADNMFLAWTV DVKGLLRAGE
NEILVKFRSV VTEDLPKLEQ LGYDLPAPND QSELGGLEEK RISVFARKAP YHYGWDWGPR
FLTSGIWREA VLTGRNAAAI ADVYIRQDEV KEASARLTAI VEVDAPDSWA GTLRMTVEGQ
EWTREVTLEP GKGVVELDLV IDQPRLWWCN GLGAPELTTF RAELLENGAV LADAEVRTGL
REIKLIRQPD AKGASFKFEL NGVPVFAKGA NHIPNDSFIT EVSEERYRHE IASAAESNMN
MLRVWGGGFY EEEVFYRLCD EYGLLVWQDF MFACSMYPGD EVFLENVRKE AEYNVRRLRN
HPCIALWCGN NEIDSAWAQF EENKGWGWKE KLSGEVRETL WTAYEEIFHR ILPEAVTAHH
PGIDYWPSSP LRELTNDADQ HSVRIAGDGD VHYWGVWHGI EPFENYNVKV GRFMSEYGFQ
SFPELKSVLT YAEEKDMELE SEVMLAHQKN GRGNQIIKEY MDIYLPEAKD FKSFLYMSQI
LQAEAMRMAI ESHRRNKPYC MGTLYWQMND CWPVASWAGM DYYGRWKALQ YTARRSFNDV
MLSVEVTDGD NVQVHAVSDL PGALAGALVL RLHAFSGAVL KEWKQSVQLA ADSSAVVFTV
PLAELMAGLD AKQVVLAASL LAEDGTLLER KEHYFAAAKE LTLEQPVLTV AEVPGSGGLS
FTVSTDVLAR GVYLTAEQEG IFSDNYFDLL PGQVKTVQFL RRRADAAAGA GMETGAGAGV
ETVETGAKAG AGAGVATGVK AGAGVPYFVP AAPQGLEVRS MADYVN
//
