ID   A0A089WUQ0_9PSED        Unreviewed;       862 AA.
AC   A0A089WUQ0;
DT   26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT   26-NOV-2014, sequence version 1.
DT   24-JAN-2024, entry version 32.
DE   RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE            EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN   ORFNames=LK03_13680 {ECO:0000313|EMBL:AIR90282.1};
OS   Pseudomonas cremoricolorata.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=157783 {ECO:0000313|EMBL:AIR90282.1, ECO:0000313|Proteomes:UP000029493};
RN   [1] {ECO:0000313|EMBL:AIR90282.1, ECO:0000313|Proteomes:UP000029493}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ND07 {ECO:0000313|EMBL:AIR90282.1,
RC   ECO:0000313|Proteomes:UP000029493};
RA   Chan K.-G.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC         ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC         Evidence={ECO:0000256|ARBA:ARBA00023501};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC       {ECO:0000256|ARBA:ARBA00007185}.
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DR   EMBL; CP009455; AIR90282.1; -; Genomic_DNA.
DR   RefSeq; WP_038412888.1; NZ_CP009455.1.
DR   AlphaFoldDB; A0A089WUQ0; -.
DR   STRING; 157783.LK03_13680; -.
DR   KEGG; psw:LK03_13680; -.
DR   eggNOG; COG1048; Bacteria.
DR   OrthoDB; 9764318at2; -.
DR   Proteomes; UP000029493; Chromosome.
DR   GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR   Gene3D; 6.10.190.10; -; 1.
DR   Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR   Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR   InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR006249; Aconitase/IRP2.
DR   InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR   PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR   PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   PRINTS; PR00415; ACONITASE.
DR   SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR   SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000029493}.
FT   DOMAIN          66..533
FT                   /note="Aconitase/3-isopropylmalate dehydratase large
FT                   subunit alpha/beta/alpha"
FT                   /evidence="ECO:0000259|Pfam:PF00330"
FT   DOMAIN          656..787
FT                   /note="Aconitase A/isopropylmalate dehydratase small
FT                   subunit swivel"
FT                   /evidence="ECO:0000259|Pfam:PF00694"
SQ   SEQUENCE   862 AA;  94028 MW;  0FB9AD9A5E3FACFC CRC64;
     MNTAYRNALP GTALDYFDAR AAVEAIQPGA YARLPYTSRV LAENLVRRCD PATLDASLGQ
     LIERKRDLDF PWYPARVVCH DILGQTALVD LAGLRDAIAD KGGDPAQVNP VVPVQLIVDH
     SLAVECGGYD PQAFERNRAI EDRRNEDRFH FINWTKQAFK NVDVIPPGNG IMHQINLEKM
     SPVIHSEQGV AYPDTCVGTD SHTPHVDALG VIAIGVGGLE AENVMLGRAS WMRLPEIVGV
     ELRGRLAPNV TATDLVLALT EYLRQQKVVG AYLEFHGSGA AALTLGDRAT ISNMAPEYGA
     TAAMFAIDQQ TLDYLRLTGR EEQQVQLVET YAKVAGLWAD SLVEADYERV LSFDLGSVVR
     NLAGPSNPHA RVATSDLAAK GIAGAWDDVP GQMPDGAVII AAITSCTNTS NPRNVIAAGL
     LARNANRLGL SRKPWVKSSL APGSRAVQLY LQEAGLEQEL EQLGFGIVAF ACTTCNGMSG
     ALDAQIQQEI IDRDLYATAV LSGNRNFDGR IHPYAKQAFL ASPPLVVAYA IAGTIRFDIE
     KDVLGVVDGR EIRLKDIWPS DAEIDAMVKA AVKPEQFRQV YIPMFAIDSH SGPSVPPLYQ
     WRPMSTYIRR PPYWEGALAG ERSLTGMRPL AVLPDNITTD HLSPSNAIMR DSAAGEYLAK
     MGLPEEDFNS YATHRGDHLT AQRATFANPK LFNEMVRDTD GKVRQGSLTR LEPEGQVTRM
     WEAIETYMAR KQPLIIVAGG DYGQGSSRDW AAKGVRLAGV EAIVAEGFER IHRTNLVGMG
     VLPLEFHPGT DRHTLALDGS ETYDVVGQRT PRATLTLVIT RRDGERLEVP VTCRLDTGEE
     VSIYEAGGVL QRFAQDFLEA TV
//