ID A0A090DCH9_PODAN Unreviewed; 2149 AA.
AC A0A090DCH9;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
OS Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)
OS (Pleurage anserina).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Podosporaceae; Podospora;
OC Podospora anserina.
OX NCBI_TaxID=515849 {ECO:0000313|EMBL:CDP32558.1, ECO:0000313|Proteomes:UP000001197};
RN [1] {ECO:0000313|EMBL:CDP32558.1, ECO:0000313|Proteomes:UP000001197}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383
RC {ECO:0000313|Proteomes:UP000001197};
RX PubMed=18460219; DOI=10.1186/gb-2008-9-5-r77;
RA Espagne E., Lespinet O., Malagnac F., Da Silva C., Jaillon O., Porcel B.M.,
RA Couloux A., Aury J.-M., Segurens B., Poulain J., Anthouard V.,
RA Grossetete S., Khalili H., Coppin E., Dequard-Chablat M., Picard M.,
RA Contamine V., Arnaise S., Bourdais A., Berteaux-Lecellier V., Gautheret D.,
RA de Vries R.P., Battaglia E., Coutinho P.M., Danchin E.G.J., Henrissat B.,
RA El Khoury R., Sainsard-Chanet A., Boivin A., Pinan-Lucarre B., Sellem C.H.,
RA Debuchy R., Wincker P., Weissenbach J., Silar P.;
RT "The genome sequence of the model ascomycete fungus Podospora anserina.";
RL Genome Biol. 9:R77.1-R77.22(2008).
RN [2] {ECO:0000313|Proteomes:UP000001197}
RP GENOME REANNOTATION.
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383
RC {ECO:0000313|Proteomes:UP000001197};
RX PubMed=24558260; DOI=10.1534/genetics.113.159988;
RA Grognet P., Bidard F., Kuchly C., Tong L.C.H., Coppin E., Benkhali J.A.,
RA Couloux A., Wincker P., Debuchy R., Silar P.;
RT "Maintaining two mating types: Structure of the mating type locus and its
RT role in heterokaryosis in Podospora anserina.";
RL Genetics 197:421-432(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR EMBL; FO904942; CDP32558.1; -; Genomic_DNA.
DR STRING; 515849.A0A090DCH9; -.
DR eggNOG; KOG1849; Eukaryota.
DR InParanoid; A0A090DCH9; -.
DR Proteomes; UP000001197; Chromosome 7.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR SUPFAM; SSF48452; TPR-like; 2.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000001197}.
FT DOMAIN 1947..2044
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
FT REGION 41..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1479..1499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..194
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2149 AA; 232898 MW; 037E56E8034F189A CRC64;
MDTNNNNATT SQTAAAAVRS AVASVATCTP ATSALLKSLL LPKEESSSSS STPAVDEPPS
RTTSRAATAR SRANTATSNV SRAPSRRGKT PAATPPVDTA GGLSSRDKAT LATQVINAAL
KALSEAAKAS STVTPATEDP PPSPSLVKTA TTRQTLRRTS SAPISPLQPR PLNRQSTSPS
VPRHVRSPSK PSLLGSSPNL LSLVECARVA LSTLRQIVTA GKVTLPELQL ESGISALVSR
LITLGLHEQA IRELRTLKRR LETLSGNEKK APVTEPKTTA EAFSEIFDFG SVKLSGPALL
LAVTTQIQAL RVLGLGKKPS AIEAVVTHLG YDKTCSPVRL LLQAAAEKGA DKGKLGRQME
TAAQCLLALC PSVSGKDDGV ATEQRLSVSP GTALELQVLG LEMRLQWWEM VKHRGDVEKE
VMVPLSRFLG AFIRRTAADA KTSYKVVVGA WERVDSRVRG LNLKPKVGSR MPLAMIYQTL
ATLAKEGGLV KEAVGWGVRL RDMIGGEGSA ESVVKKVSVA AQLLGCQLKD RTKYLHDDGL
IGEVVAGMMG PLRGDMSELD ELLGNVVQAR KAAISVLVVE RKSEVSSLQK GTRDQLEMLV
LQTPRFCLRW LGKPPGPKGS TKDYLRYEQR RQLLQGSLQQ NLESAFLVVK VRLEEGRMAW
EVTDSVLGDC VTLLEYTGSS NGGGEGSSMY VKISHFYYLQ YNALRQTIAD PKDPSPLRAL
RRSVDCVKYR SGKEKEKAQL ILKLERQSEL AKRLGRGEEA ISALFAIRTS LLEDGVLATV
AKGLATQSPV AVWERDEKTE ALSRALVAIA QMEQVWMDWT VDLSEAERAA ALEHRLRYVL
LRAGSSYTDV TLEHPCVDQL LRIYIPTRYP IRRLRVLLSL LCAALGKLDK AEELLAVAKD
ASQVEDLGED TGLVGYLQHM KALFGSLAAA VDGYKNAGAL RESVVVWQGI IKSCQDKAAL
EKQVDDVAGL LDYLQSIADF LRMKGKDEML ASVLELVTDI SQVAATQGSK AEELIQHNSA
LAMQYTNLGR STKAEQLFTK ARQHLGPQAS GDTTAAFYLA CAEHAVALGD FQQAEEHLAS
ARTAYTTSST STYTRLQKKQ LVAYAFYLHS ILAQEKGDSH HALVYSRESV RAIMAEWVKL
EAQLVASSIT SSSSSSPDQT DLSLATATQH LPVAASGPAF WKMFHGLFRN TMRLSFVYAH
LGLFQETMYY AEQAGKIAEQ VDSEFYNCAV AGWMGEVSWR SGGIEKAGEM LGKATGLFTE
VAGGGNWFGA AELAVRLSEI CVGVGNLKGG GWLLERAEGI VKGLVKDKGV EDVEEGMKKL
KIEEGSKGTT ARGGVGGRKV VGGGGVKKPV RKTGAAGARA AATKAKPVVK AAAPAPEPVV
VVEDAQLAKL RASILVQKAV LMLKQKEWAG AQSILSEAAS ATTVKSSDLL PTRQLAMASC
LLGMSMEQMA QDPVFSVIQD STISFPALSG MERLSSVQAS PMKAATSPKK GARAAGKGEG
AKDLSQGVYV DNLREAHDYL LEAHSVASKS GDSALIHKIS GMLQHVGLFL TATSSSRTKA
TIHSAQTSYS VELARNLIWR RERKAMIQEK HAPRHDGTEW PPALQSVTSR RSSLGFTLDL
HKVQRDYIDI VPKNWNVISI ALSEGNQDLC ITKLQAGQNP FVIRLPLERA TSRDADSDVF
NFQQGRSELL DIIKEINATC HSAKDMNQKG AKTEWWNARE ALDERLKELL ENIEQIWLGG
FRGIFSQHCR RADLLARFQK SFLAMMDKHL PSRRQVRGKK TKAAAAGQKV QLDLNILELF
IGLGDATRPG TDFDDELTDL LYFVVDILQF HGERNAYDEI DFDSMVVETF DALMGYHSAV
NEGRSRSSRG GEEETGIHTV LLLDKALHVF PWESLPCMQG LAISRMPSLA CLRRLILEQR
LSEPPVGEGG GGLLDVEGHH VDFGKGKGKG AYILNPSGDL AATQKTFGPA LEENLSSFES
IISRPPTEPE FERFLAEKDL LLYFGHGSGA QYIRGRTIRR LDRCKAAVLL MGCSSAALTE
AGEFEPSGPA WNYMLSGSPA VVGTLWDVTD RDIDRFAGRM LETWGVLPKG AVEDDGKGRD
TGKGKRARGD KKRSLVEAVA EAREGGCRFR YVTAAGVVVY GVPVYVWKE
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