ID A0A090J0F2_9BACI Unreviewed; 312 AA.
AC A0A090J0F2;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase H {ECO:0000256|HAMAP-Rule:MF_01007};
DE EC=2.1.1.199 {ECO:0000256|HAMAP-Rule:MF_01007};
DE AltName: Full=16S rRNA m(4)C1402 methyltransferase {ECO:0000256|HAMAP-Rule:MF_01007};
DE AltName: Full=rRNA (cytosine-N(4)-)-methyltransferase RsmH {ECO:0000256|HAMAP-Rule:MF_01007};
GN Name=rsmH {ECO:0000256|HAMAP-Rule:MF_01007,
GN ECO:0000313|EMBL:CEE01300.1};
GN ORFNames=B4064_2523 {ECO:0000313|EMBL:KIO65740.1}, B4065_0925
GN {ECO:0000313|EMBL:KIO59104.1}, B4166_1448
GN {ECO:0000313|EMBL:KIO70823.1}, B4167_1540
GN {ECO:0000313|EMBL:KIO74255.1}, BT1A1_1471
GN {ECO:0000313|EMBL:CEE01300.1};
OS Caldibacillus thermoamylovorans.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Caldibacillus.
OX NCBI_TaxID=35841 {ECO:0000313|EMBL:CEE01300.1, ECO:0000313|Proteomes:UP000040576};
RN [1] {ECO:0000313|EMBL:CEE01300.1, ECO:0000313|Proteomes:UP000040576}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Wibberg Daniel;
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000032071, ECO:0000313|Proteomes:UP000032076}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B4064 {ECO:0000313|EMBL:KIO65740.1,
RC ECO:0000313|Proteomes:UP000032078}, B4065
RC {ECO:0000313|EMBL:KIO59104.1, ECO:0000313|Proteomes:UP000032071},
RC B4166 {ECO:0000313|EMBL:KIO70823.1,
RC ECO:0000313|Proteomes:UP000032097}, and B4167
RC {ECO:0000313|EMBL:KIO74255.1, ECO:0000313|Proteomes:UP000032076};
RA Krawcyk A.O., Berendsen E.M., Eijlander R.T., de Jong A., Wells-Bennik M.,
RA Kuipers O.P.;
RT "Draft Genome Sequences of Four Bacillus thermoamylovorans Strains,
RT Isolated From Food Products.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Specifically methylates the N4 position of cytidine in
CC position 1402 (C1402) of 16S rRNA. {ECO:0000256|HAMAP-Rule:MF_01007}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42928, Rhea:RHEA-COMP:10286, Rhea:RHEA-COMP:10287,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74506, ChEBI:CHEBI:82748; EC=2.1.1.199;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01007};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01007}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmH family.
CC {ECO:0000256|ARBA:ARBA00010396, ECO:0000256|HAMAP-Rule:MF_01007}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CCRF01000045; CEE01300.1; -; Genomic_DNA.
DR EMBL; JXLS01000131; KIO59104.1; -; Genomic_DNA.
DR EMBL; JXLR01000058; KIO65740.1; -; Genomic_DNA.
DR EMBL; JXLT01000006; KIO70823.1; -; Genomic_DNA.
DR EMBL; JXLU01000009; KIO74255.1; -; Genomic_DNA.
DR RefSeq; WP_034769601.1; NZ_JXLU01000009.1.
DR AlphaFoldDB; A0A090J0F2; -.
DR STRING; 35841.B4167_1540; -.
DR PATRIC; fig|35841.6.peg.2055; -.
DR eggNOG; COG0275; Bacteria.
DR OrthoDB; 9806637at2; -.
DR Proteomes; UP000032071; Unassembled WGS sequence.
DR Proteomes; UP000032076; Unassembled WGS sequence.
DR Proteomes; UP000032078; Unassembled WGS sequence.
DR Proteomes; UP000032097; Unassembled WGS sequence.
DR Proteomes; UP000040576; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0071424; F:rRNA (cytosine-N4-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0070475; P:rRNA base methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.170; Putative methyltransferase TM0872, insert domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR HAMAP; MF_01007; 16SrRNA_methyltr_H; 1.
DR InterPro; IPR002903; RsmH.
DR InterPro; IPR023397; SAM-dep_MeTrfase_MraW_recog.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00006; 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH; 1.
DR PANTHER; PTHR11265:SF0; 12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11265; S-ADENOSYL-METHYLTRANSFERASE MRAW; 1.
DR Pfam; PF01795; Methyltransf_5; 1.
DR PIRSF; PIRSF004486; MraW; 1.
DR SUPFAM; SSF81799; Putative methyltransferase TM0872, insert domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01007};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|HAMAP-
KW Rule:MF_01007}; Reference proteome {ECO:0000313|Proteomes:UP000040576};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552, ECO:0000256|HAMAP-
KW Rule:MF_01007};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01007};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01007}.
FT BINDING 32..34
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01007"
FT BINDING 52
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01007"
FT BINDING 79
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01007"
FT BINDING 100
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01007"
FT BINDING 107
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01007"
SQ SEQUENCE 312 AA; 35775 MW; 69D477D9C3EBEF00 CRC64;
MFQHTTVLLR EAVEGLNIKP DGIYVDCTLG GAGHTEEILK RLSEKGKVYA FDQDLTAVEN
AKKKLAIYGN RLEIIHRNFA HLKEELHIRN VFQVDGILYD LGVSSPQLDE PDRGFSYHHD
ALLDMRMDQH SPISAKEVVN EWPYEKLVKI FFKYGEEKFS KQIARNIERF REKQTIETTG
QLVEIIKESI PLPARRKGGH PAKRIFQAIR IAVNDELNSF EKSLEQSIEL LKVGGRTAVI
TFHSLEDRIT KTIFKKYATI PDLPSGLPII PDEYQPVLKV ITRKPIVPSE EELIRNNRAR
SAKLRIAEKI KD
//
