ID A0A091IU25_EGRGA Unreviewed; 332 AA.
AC A0A091IU25;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Lactosylceramide 4-alpha-galactosyltransferase {ECO:0000256|ARBA:ARBA00040835};
DE EC=2.4.1.228 {ECO:0000256|ARBA:ARBA00039051};
DE AltName: Full=Alpha-1,4-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00043186};
DE AltName: Full=Alpha-1,4-galactosyltransferase {ECO:0000256|ARBA:ARBA00041849};
DE AltName: Full=Globotriaosylceramide synthase {ECO:0000256|ARBA:ARBA00043154};
DE AltName: Full=UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase {ECO:0000256|ARBA:ARBA00041556};
DE Flags: Fragment;
GN ORFNames=Z169_15604 {ECO:0000313|EMBL:KFP11058.1};
OS Egretta garzetta (Little egret).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Pelecaniformes; Ardeidae; Egretta.
OX NCBI_TaxID=188379 {ECO:0000313|EMBL:KFP11058.1, ECO:0000313|Proteomes:UP000053119};
RN [1] {ECO:0000313|EMBL:KFP11058.1, ECO:0000313|Proteomes:UP000053119}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_Z169 {ECO:0000313|EMBL:KFP11058.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-
CC alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H(+) + UDP;
CC Xref=Rhea:RHEA:11924, ChEBI:CHEBI:15378, ChEBI:CHEBI:17950,
CC ChEBI:CHEBI:18313, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914;
CC EC=2.4.1.228; Evidence={ECO:0000256|ARBA:ARBA00043770};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11925;
CC Evidence={ECO:0000256|ARBA:ARBA00043770};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-
CC (1->4)-beta-D-Gal-(1<->1')-Cer + H(+) + UDP; Xref=Rhea:RHEA:60044,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914,
CC ChEBI:CHEBI:143593, ChEBI:CHEBI:143594;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60045;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC -!- PATHWAY: Glycolipid biosynthesis. {ECO:0000256|ARBA:ARBA00004934}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
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DR EMBL; KK500858; KFP11058.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A091IU25; -.
DR Proteomes; UP000053119; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProt.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.20; -; 1.
DR InterPro; IPR007652; A1-4-GlycosylTfrase_dom.
DR InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR12042:SF17; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE; 1.
DR PANTHER; PTHR12042; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE; 1.
DR Pfam; PF04572; Gb3_synth; 1.
DR Pfam; PF04488; Gly_transf_sug; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 4: Predicted;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000053119};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT SIGNAL 1..17
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 18..332
FT /note="Lactosylceramide 4-alpha-galactosyltransferase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001876674"
FT DOMAIN 203..327
FT /note="Alpha 1,4-glycosyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04572"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFP11058.1"
FT NON_TER 332
FT /evidence="ECO:0000313|EMBL:KFP11058.1"
SQ SEQUENCE 332 AA; 37887 MW; 3D1F09BD412DB7A1 CRC64;
LFILTISFVF FASVVFYWRT GNDAEAQLYS FHTQSKCAEF LPYLPHPTAG GPPPSPGDVF
FVETSEKTDP SYLFTCSVES AARTHPGTRV VVLMKGLANG NASLPNHWAF SLLSCFPNVE
FRHLDLPELF SGTPLAKWYL QSEHQKEEHF LAVLSDACRI TIMWKFGGIY LDTDFIVLRN
LKNLTNALGI QSQDVLNGAF LSFKPKHEFM ELCMKDFVEN YNGQIWGHQG PELLTRVFKK
WCPISYIQNN MSCKGVRALS PEVLYPIFWG DWKKLFDTVN SSELHRLLKN TYAVHVWNKL
SHGTRLEITS QAFLAQLYSQ FCPATSTKMK RD
//
